High-resolution mass spectrometry of small molecules bound to membrane proteins

Small molecules are known to stabilise membrane proteins and to modulate function and oligomeric state, but their identity is often hard to define. Here we develop and apply a high-resolution, Orbitrap mass spectrometer for intact membrane protein-ligand complexes. Using this platform we resolve the...

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Autores principales: Gault, Joseph, Donlan, Joseph A. C., Liko, Idlir, Hopper, Jonathan T.S., Gupta, Kallol, Housden, Nicholas G., Struwe, Weston B., Marty, Michael T., Mize, Todd, Bechara, Cherine, Zhu, Ya, Wu, Beili, Kleanthous, Colin, Belov, Mikhail, Damoc, Eugen, Makarov, Alexander, Robinson, Carol V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856209/
https://www.ncbi.nlm.nih.gov/pubmed/26901650
http://dx.doi.org/10.1038/nmeth.3771
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author Gault, Joseph
Donlan, Joseph A. C.
Liko, Idlir
Hopper, Jonathan T.S.
Gupta, Kallol
Housden, Nicholas G.
Struwe, Weston B.
Marty, Michael T.
Mize, Todd
Bechara, Cherine
Zhu, Ya
Wu, Beili
Kleanthous, Colin
Belov, Mikhail
Damoc, Eugen
Makarov, Alexander
Robinson, Carol V.
author_facet Gault, Joseph
Donlan, Joseph A. C.
Liko, Idlir
Hopper, Jonathan T.S.
Gupta, Kallol
Housden, Nicholas G.
Struwe, Weston B.
Marty, Michael T.
Mize, Todd
Bechara, Cherine
Zhu, Ya
Wu, Beili
Kleanthous, Colin
Belov, Mikhail
Damoc, Eugen
Makarov, Alexander
Robinson, Carol V.
author_sort Gault, Joseph
collection PubMed
description Small molecules are known to stabilise membrane proteins and to modulate function and oligomeric state, but their identity is often hard to define. Here we develop and apply a high-resolution, Orbitrap mass spectrometer for intact membrane protein-ligand complexes. Using this platform we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length.
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spelling pubmed-48562092016-09-22 High-resolution mass spectrometry of small molecules bound to membrane proteins Gault, Joseph Donlan, Joseph A. C. Liko, Idlir Hopper, Jonathan T.S. Gupta, Kallol Housden, Nicholas G. Struwe, Weston B. Marty, Michael T. Mize, Todd Bechara, Cherine Zhu, Ya Wu, Beili Kleanthous, Colin Belov, Mikhail Damoc, Eugen Makarov, Alexander Robinson, Carol V. Nat Methods Article Small molecules are known to stabilise membrane proteins and to modulate function and oligomeric state, but their identity is often hard to define. Here we develop and apply a high-resolution, Orbitrap mass spectrometer for intact membrane protein-ligand complexes. Using this platform we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length. 2016-02-22 2016-04 /pmc/articles/PMC4856209/ /pubmed/26901650 http://dx.doi.org/10.1038/nmeth.3771 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Gault, Joseph
Donlan, Joseph A. C.
Liko, Idlir
Hopper, Jonathan T.S.
Gupta, Kallol
Housden, Nicholas G.
Struwe, Weston B.
Marty, Michael T.
Mize, Todd
Bechara, Cherine
Zhu, Ya
Wu, Beili
Kleanthous, Colin
Belov, Mikhail
Damoc, Eugen
Makarov, Alexander
Robinson, Carol V.
High-resolution mass spectrometry of small molecules bound to membrane proteins
title High-resolution mass spectrometry of small molecules bound to membrane proteins
title_full High-resolution mass spectrometry of small molecules bound to membrane proteins
title_fullStr High-resolution mass spectrometry of small molecules bound to membrane proteins
title_full_unstemmed High-resolution mass spectrometry of small molecules bound to membrane proteins
title_short High-resolution mass spectrometry of small molecules bound to membrane proteins
title_sort high-resolution mass spectrometry of small molecules bound to membrane proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856209/
https://www.ncbi.nlm.nih.gov/pubmed/26901650
http://dx.doi.org/10.1038/nmeth.3771
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