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A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydr...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856383/ https://www.ncbi.nlm.nih.gov/pubmed/27144306 http://dx.doi.org/10.1371/journal.pone.0154610 |
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author | Massai, Diana Isu, Giuseppe Madeddu, Denise Cerino, Giulia Falco, Angela Frati, Caterina Gallo, Diego Deriu, Marco A. Falvo D’Urso Labate, Giuseppe Quaini, Federico Audenino, Alberto Morbiducci, Umberto |
author_facet | Massai, Diana Isu, Giuseppe Madeddu, Denise Cerino, Giulia Falco, Angela Frati, Caterina Gallo, Diego Deriu, Marco A. Falvo D’Urso Labate, Giuseppe Quaini, Federico Audenino, Alberto Morbiducci, Umberto |
author_sort | Massai, Diana |
collection | PubMed |
description | A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future. |
format | Online Article Text |
id | pubmed-4856383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48563832016-05-06 A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids Massai, Diana Isu, Giuseppe Madeddu, Denise Cerino, Giulia Falco, Angela Frati, Caterina Gallo, Diego Deriu, Marco A. Falvo D’Urso Labate, Giuseppe Quaini, Federico Audenino, Alberto Morbiducci, Umberto PLoS One Research Article A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future. Public Library of Science 2016-05-04 /pmc/articles/PMC4856383/ /pubmed/27144306 http://dx.doi.org/10.1371/journal.pone.0154610 Text en © 2016 Massai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Massai, Diana Isu, Giuseppe Madeddu, Denise Cerino, Giulia Falco, Angela Frati, Caterina Gallo, Diego Deriu, Marco A. Falvo D’Urso Labate, Giuseppe Quaini, Federico Audenino, Alberto Morbiducci, Umberto A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids |
title | A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids |
title_full | A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids |
title_fullStr | A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids |
title_full_unstemmed | A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids |
title_short | A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids |
title_sort | versatile bioreactor for dynamic suspension cell culture. application to the culture of cancer cell spheroids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856383/ https://www.ncbi.nlm.nih.gov/pubmed/27144306 http://dx.doi.org/10.1371/journal.pone.0154610 |
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