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A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids

A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydr...

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Autores principales: Massai, Diana, Isu, Giuseppe, Madeddu, Denise, Cerino, Giulia, Falco, Angela, Frati, Caterina, Gallo, Diego, Deriu, Marco A., Falvo D’Urso Labate, Giuseppe, Quaini, Federico, Audenino, Alberto, Morbiducci, Umberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856383/
https://www.ncbi.nlm.nih.gov/pubmed/27144306
http://dx.doi.org/10.1371/journal.pone.0154610
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author Massai, Diana
Isu, Giuseppe
Madeddu, Denise
Cerino, Giulia
Falco, Angela
Frati, Caterina
Gallo, Diego
Deriu, Marco A.
Falvo D’Urso Labate, Giuseppe
Quaini, Federico
Audenino, Alberto
Morbiducci, Umberto
author_facet Massai, Diana
Isu, Giuseppe
Madeddu, Denise
Cerino, Giulia
Falco, Angela
Frati, Caterina
Gallo, Diego
Deriu, Marco A.
Falvo D’Urso Labate, Giuseppe
Quaini, Federico
Audenino, Alberto
Morbiducci, Umberto
author_sort Massai, Diana
collection PubMed
description A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future.
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spelling pubmed-48563832016-05-06 A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids Massai, Diana Isu, Giuseppe Madeddu, Denise Cerino, Giulia Falco, Angela Frati, Caterina Gallo, Diego Deriu, Marco A. Falvo D’Urso Labate, Giuseppe Quaini, Federico Audenino, Alberto Morbiducci, Umberto PLoS One Research Article A versatile bioreactor suitable for dynamic suspension cell culture under tunable shear stress conditions has been developed and preliminarily tested culturing cancer cell spheroids. By adopting simple technological solutions and avoiding rotating components, the bioreactor exploits the laminar hydrodynamics establishing within the culture chamber enabling dynamic cell suspension in an environment favourable to mass transport, under a wide range of tunable shear stress conditions. The design phase of the device has been supported by multiphysics modelling and has provided a comprehensive analysis of the operating principles of the bioreactor. Moreover, an explanatory example is herein presented with multiphysics simulations used to set the proper bioreactor operating conditions for preliminary in vitro biological tests on a human lung carcinoma cell line. The biological results demonstrate that the ultralow shear dynamic suspension provided by the device is beneficial for culturing cancer cell spheroids. In comparison to the static suspension control, dynamic cell suspension preserves morphological features, promotes intercellular connection, increases spheroid size (2.4-fold increase) and number of cycling cells (1.58-fold increase), and reduces double strand DNA damage (1.5-fold reduction). It is envisioned that the versatility of this bioreactor could allow investigation and expansion of different cell types in the future. Public Library of Science 2016-05-04 /pmc/articles/PMC4856383/ /pubmed/27144306 http://dx.doi.org/10.1371/journal.pone.0154610 Text en © 2016 Massai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Massai, Diana
Isu, Giuseppe
Madeddu, Denise
Cerino, Giulia
Falco, Angela
Frati, Caterina
Gallo, Diego
Deriu, Marco A.
Falvo D’Urso Labate, Giuseppe
Quaini, Federico
Audenino, Alberto
Morbiducci, Umberto
A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
title A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
title_full A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
title_fullStr A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
title_full_unstemmed A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
title_short A Versatile Bioreactor for Dynamic Suspension Cell Culture. Application to the Culture of Cancer Cell Spheroids
title_sort versatile bioreactor for dynamic suspension cell culture. application to the culture of cancer cell spheroids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856383/
https://www.ncbi.nlm.nih.gov/pubmed/27144306
http://dx.doi.org/10.1371/journal.pone.0154610
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