A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate

Elevated reaction temperatures are crucial for the efficient enzymatic degradation of polyethylene terephthalate (PET). A disulfide bridge was introduced to the polyester hydrolase TfCut2 to substitute its calcium binding site. The melting point of the resulting variant increased to 94.7 °C (wild‐ty...

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Detalles Bibliográficos
Autores principales: Then, Johannes, Wei, Ren, Oeser, Thorsten, Gerdts, André, Schmidt, Juliane, Barth, Markus, Zimmermann, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856421/
https://www.ncbi.nlm.nih.gov/pubmed/27419048
http://dx.doi.org/10.1002/2211-5463.12053

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856421/
https://www.ncbi.nlm.nih.gov/pubmed/27419048
http://dx.doi.org/10.1002/2211-5463.12053

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