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Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)

Abstract. Karyotype analysis was performed on the scleractinian coral Coelastrea aspera Verrill, 1866, commonly found along temperate coasts in Japan (30–35°N) and in coastal waters in the Indian and Pacific oceans. G-banding of Coelastrea aspera was successfully performed, although the banding patt...

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Autores principales: Taguchi, Takahiro, Kubota, Satoshi, Mezaki, Takuma, Tagami, Erika, Sekida, Satoko, Nakachi, Shu, Okuda, Kazuo, Tominaga, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856926/
https://www.ncbi.nlm.nih.gov/pubmed/27186338
http://dx.doi.org/10.3897/CompCytogen.v10i1.5699
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author Taguchi, Takahiro
Kubota, Satoshi
Mezaki, Takuma
Tagami, Erika
Sekida, Satoko
Nakachi, Shu
Okuda, Kazuo
Tominaga, Akira
author_facet Taguchi, Takahiro
Kubota, Satoshi
Mezaki, Takuma
Tagami, Erika
Sekida, Satoko
Nakachi, Shu
Okuda, Kazuo
Tominaga, Akira
author_sort Taguchi, Takahiro
collection PubMed
description Abstract. Karyotype analysis was performed on the scleractinian coral Coelastrea aspera Verrill, 1866, commonly found along temperate coasts in Japan (30–35°N) and in coastal waters in the Indian and Pacific oceans. G-banding of Coelastrea aspera was successfully performed, although the banding pattern was not as clear as that in mammals. The karyogram clearly revealed that this coral had a (hsr) in chromosome 11. This hsr consisted of (rRNA) related genes, which was demonstrated by (FISH) with probes generated using 28S (rDNA) primers and those generated through chromosome microdissection. In addition, we conducted (Ag-NOR) analysis and found Ag depositions in the interphase nuclei but not on rRNA gene loci and hsr(s) in the mitotic stage. The hsr of this coral was observed in approximately 50% of the metaphase spreads analyzed. This may explain the diversity of coral rDNA based on the molecular study of sequence analysis. Furthermore, it was discovered that human telomere and Alu repeated sequences were present in this Coelastrea aspera. Probes derived from human Alu sequences are expected to play an important role in the classification of corals. Overall, our data can be of great value in discriminating among scleractinian coral species and understanding their genetics, including chromosomal evolution.
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spelling pubmed-48569262016-05-16 Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria) Taguchi, Takahiro Kubota, Satoshi Mezaki, Takuma Tagami, Erika Sekida, Satoko Nakachi, Shu Okuda, Kazuo Tominaga, Akira Comp Cytogenet Research Articles Abstract. Karyotype analysis was performed on the scleractinian coral Coelastrea aspera Verrill, 1866, commonly found along temperate coasts in Japan (30–35°N) and in coastal waters in the Indian and Pacific oceans. G-banding of Coelastrea aspera was successfully performed, although the banding pattern was not as clear as that in mammals. The karyogram clearly revealed that this coral had a (hsr) in chromosome 11. This hsr consisted of (rRNA) related genes, which was demonstrated by (FISH) with probes generated using 28S (rDNA) primers and those generated through chromosome microdissection. In addition, we conducted (Ag-NOR) analysis and found Ag depositions in the interphase nuclei but not on rRNA gene loci and hsr(s) in the mitotic stage. The hsr of this coral was observed in approximately 50% of the metaphase spreads analyzed. This may explain the diversity of coral rDNA based on the molecular study of sequence analysis. Furthermore, it was discovered that human telomere and Alu repeated sequences were present in this Coelastrea aspera. Probes derived from human Alu sequences are expected to play an important role in the classification of corals. Overall, our data can be of great value in discriminating among scleractinian coral species and understanding their genetics, including chromosomal evolution. Pensoft Publishers 2016-01-22 /pmc/articles/PMC4856926/ /pubmed/27186338 http://dx.doi.org/10.3897/CompCytogen.v10i1.5699 Text en Takahiro Taguchi, Satoshi Kubota, Takuma Mezaki, Erika Tagami, Satoko Sekida, Shu Nakachi, Kazuo Okuda, Akira Tominaga http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Articles
Taguchi, Takahiro
Kubota, Satoshi
Mezaki, Takuma
Tagami, Erika
Sekida, Satoko
Nakachi, Shu
Okuda, Kazuo
Tominaga, Akira
Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)
title Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)
title_full Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)
title_fullStr Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)
title_full_unstemmed Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)
title_short Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)
title_sort identification of homogeneously staining regions by g-banding and chromosome microdissection, and fish marker selection using human alu sequence primers in a scleractinian coral coelastrea aspera verrill, 1866 (cnidaria)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856926/
https://www.ncbi.nlm.nih.gov/pubmed/27186338
http://dx.doi.org/10.3897/CompCytogen.v10i1.5699
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