Cargando…

Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt

Damaged DNA can be repaired by removal and re-synthesis of up to 30 nucleotides during base or nucleotide excision repair. An important question is what happens when many more nucleotides are removed, resulting in long single-stranded DNA (ssDNA) lesions. Such lesions appear on chromosomes during te...

Descripción completa

Detalles Bibliográficos
Autores principales: Ivanova, Iglika G., Maringele, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856982/
https://www.ncbi.nlm.nih.gov/pubmed/26883631
http://dx.doi.org/10.1093/nar/gkw071
_version_ 1782430576856268800
author Ivanova, Iglika G.
Maringele, Laura
author_facet Ivanova, Iglika G.
Maringele, Laura
author_sort Ivanova, Iglika G.
collection PubMed
description Damaged DNA can be repaired by removal and re-synthesis of up to 30 nucleotides during base or nucleotide excision repair. An important question is what happens when many more nucleotides are removed, resulting in long single-stranded DNA (ssDNA) lesions. Such lesions appear on chromosomes during telomere damage, double strand break repair or after the UV damage of stationary phase cells. Here, we show that long single-stranded lesions, formed at dysfunctional telomeres in budding yeast, are re-synthesized when cells are removed from the telomere-damaging environment. This process requires Pol32, an accessory factor of Polymerase δ. However, re-synthesis takes place even when the telomere-damaging conditions persist, in which case the accessory factors of both polymerases δ and ε are required, and surprisingly, salt. Salt added to the medium facilitates the DNA synthesis, independently of the osmotic stress responses. These results provide unexpected insights into the DNA metabolism and challenge the current view on cellular responses to telomere dysfunction.
format Online
Article
Text
id pubmed-4856982
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-48569822016-05-09 Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt Ivanova, Iglika G. Maringele, Laura Nucleic Acids Res Genome Integrity, Repair and Replication Damaged DNA can be repaired by removal and re-synthesis of up to 30 nucleotides during base or nucleotide excision repair. An important question is what happens when many more nucleotides are removed, resulting in long single-stranded DNA (ssDNA) lesions. Such lesions appear on chromosomes during telomere damage, double strand break repair or after the UV damage of stationary phase cells. Here, we show that long single-stranded lesions, formed at dysfunctional telomeres in budding yeast, are re-synthesized when cells are removed from the telomere-damaging environment. This process requires Pol32, an accessory factor of Polymerase δ. However, re-synthesis takes place even when the telomere-damaging conditions persist, in which case the accessory factors of both polymerases δ and ε are required, and surprisingly, salt. Salt added to the medium facilitates the DNA synthesis, independently of the osmotic stress responses. These results provide unexpected insights into the DNA metabolism and challenge the current view on cellular responses to telomere dysfunction. Oxford University Press 2016-05-05 2016-02-15 /pmc/articles/PMC4856982/ /pubmed/26883631 http://dx.doi.org/10.1093/nar/gkw071 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Integrity, Repair and Replication
Ivanova, Iglika G.
Maringele, Laura
Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt
title Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt
title_full Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt
title_fullStr Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt
title_full_unstemmed Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt
title_short Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt
title_sort polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt
topic Genome Integrity, Repair and Replication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4856982/
https://www.ncbi.nlm.nih.gov/pubmed/26883631
http://dx.doi.org/10.1093/nar/gkw071
work_keys_str_mv AT ivanovaiglikag polymeraseseandrepairdysfunctionaltelomeresfacilitatedbysalt
AT maringelelaura polymeraseseandrepairdysfunctionaltelomeresfacilitatedbysalt