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An Evaluation of Blood Compatibility of Silver Nanoparticles

Silver nanoparticles (AgNPs) have tremendous potentials in medical devices due to their excellent antimicrobial properties. Blood compatibility should be investigated for AgNPs due to the potential blood contact. However, so far, most studies are not systematic and have not provided insights into th...

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Autores principales: Huang, He, Lai, Wenjia, Cui, Menghua, Liang, Ling, Lin, Yuchen, Fang, Qiaojun, Liu, Ying, Xie, Liming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857076/
https://www.ncbi.nlm.nih.gov/pubmed/27145858
http://dx.doi.org/10.1038/srep25518
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author Huang, He
Lai, Wenjia
Cui, Menghua
Liang, Ling
Lin, Yuchen
Fang, Qiaojun
Liu, Ying
Xie, Liming
author_facet Huang, He
Lai, Wenjia
Cui, Menghua
Liang, Ling
Lin, Yuchen
Fang, Qiaojun
Liu, Ying
Xie, Liming
author_sort Huang, He
collection PubMed
description Silver nanoparticles (AgNPs) have tremendous potentials in medical devices due to their excellent antimicrobial properties. Blood compatibility should be investigated for AgNPs due to the potential blood contact. However, so far, most studies are not systematic and have not provided insights into the mechanisms for blood compatibility of AgNPs. In this study, we have investigated the blood biological effects, including hemolysis, lymphocyte proliferation, platelet aggregation, coagulation and complement activation, of 20 nm AgNPs with two different surface coatings (polyvinyl pyrrolidone and citrate). Our results have revealed AgNPs could elicit hemolysis and severely impact the proliferation and viability of lymphocytes at all investigated concentrations (10, 20, 40 μg/mL). Nevertheless, AgNPs didn’t show any effect on platelet aggregation, coagulation process, or complement activation at up to ~40 μg/mL. Proteomic analysis on AgNPs plasma proteins corona has revealed that acidic and small molecular weight blood plasma proteins were preferentially adsorbed onto AgNPs, and these include some important proteins relevant to hemostasis, coagulation, platelet, complement activation and immune responses. The predicted biological effects of AgNPs by proteomic analysis are mostly consistent with our experimental data since there were few C3 components on AgNPs and more negative than positive factors involving platelet aggregation and thrombosis.
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spelling pubmed-48570762016-05-18 An Evaluation of Blood Compatibility of Silver Nanoparticles Huang, He Lai, Wenjia Cui, Menghua Liang, Ling Lin, Yuchen Fang, Qiaojun Liu, Ying Xie, Liming Sci Rep Article Silver nanoparticles (AgNPs) have tremendous potentials in medical devices due to their excellent antimicrobial properties. Blood compatibility should be investigated for AgNPs due to the potential blood contact. However, so far, most studies are not systematic and have not provided insights into the mechanisms for blood compatibility of AgNPs. In this study, we have investigated the blood biological effects, including hemolysis, lymphocyte proliferation, platelet aggregation, coagulation and complement activation, of 20 nm AgNPs with two different surface coatings (polyvinyl pyrrolidone and citrate). Our results have revealed AgNPs could elicit hemolysis and severely impact the proliferation and viability of lymphocytes at all investigated concentrations (10, 20, 40 μg/mL). Nevertheless, AgNPs didn’t show any effect on platelet aggregation, coagulation process, or complement activation at up to ~40 μg/mL. Proteomic analysis on AgNPs plasma proteins corona has revealed that acidic and small molecular weight blood plasma proteins were preferentially adsorbed onto AgNPs, and these include some important proteins relevant to hemostasis, coagulation, platelet, complement activation and immune responses. The predicted biological effects of AgNPs by proteomic analysis are mostly consistent with our experimental data since there were few C3 components on AgNPs and more negative than positive factors involving platelet aggregation and thrombosis. Nature Publishing Group 2016-05-05 /pmc/articles/PMC4857076/ /pubmed/27145858 http://dx.doi.org/10.1038/srep25518 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Huang, He
Lai, Wenjia
Cui, Menghua
Liang, Ling
Lin, Yuchen
Fang, Qiaojun
Liu, Ying
Xie, Liming
An Evaluation of Blood Compatibility of Silver Nanoparticles
title An Evaluation of Blood Compatibility of Silver Nanoparticles
title_full An Evaluation of Blood Compatibility of Silver Nanoparticles
title_fullStr An Evaluation of Blood Compatibility of Silver Nanoparticles
title_full_unstemmed An Evaluation of Blood Compatibility of Silver Nanoparticles
title_short An Evaluation of Blood Compatibility of Silver Nanoparticles
title_sort evaluation of blood compatibility of silver nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857076/
https://www.ncbi.nlm.nih.gov/pubmed/27145858
http://dx.doi.org/10.1038/srep25518
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