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Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats

Understanding the relationship between brain function and behavior remains a major challenge in neuroscience. Photoacoustic tomography (PAT) is an emerging technique that allows for noninvasive in vivo brain imaging at micrometer-millisecond spatiotemporal resolution. In this article, a novel, minia...

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Detalles Bibliográficos
Autores principales: Tang, Jianbo, Coleman, Jason E., Dai, Xianjin, Jiang, Huabei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857106/
https://www.ncbi.nlm.nih.gov/pubmed/27146026
http://dx.doi.org/10.1038/srep25470
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author Tang, Jianbo
Coleman, Jason E.
Dai, Xianjin
Jiang, Huabei
author_facet Tang, Jianbo
Coleman, Jason E.
Dai, Xianjin
Jiang, Huabei
author_sort Tang, Jianbo
collection PubMed
description Understanding the relationship between brain function and behavior remains a major challenge in neuroscience. Photoacoustic tomography (PAT) is an emerging technique that allows for noninvasive in vivo brain imaging at micrometer-millisecond spatiotemporal resolution. In this article, a novel, miniaturized 3D wearable PAT (3D-wPAT) technique is described for brain imaging in behaving rats. 3D-wPAT has three layers of fully functional acoustic transducer arrays. Phantom imaging experiments revealed that the in-plane X-Y spatial resolutions were ~200 μm for each acoustic detection layer. The functional imaging capacity of 3D-wPAT was demonstrated by mapping the cerebral oxygen saturation via multi-wavelength irradiation in behaving hyperoxic rats. In addition, we demonstrated that 3D-wPAT could be used for monitoring sensory stimulus-evoked responses in behaving rats by measuring hemodynamic responses in the primary visual cortex during visual stimulation. Together, these results show the potential of 3D-wPAT for brain study in behaving rodents.
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spelling pubmed-48571062016-05-18 Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats Tang, Jianbo Coleman, Jason E. Dai, Xianjin Jiang, Huabei Sci Rep Article Understanding the relationship between brain function and behavior remains a major challenge in neuroscience. Photoacoustic tomography (PAT) is an emerging technique that allows for noninvasive in vivo brain imaging at micrometer-millisecond spatiotemporal resolution. In this article, a novel, miniaturized 3D wearable PAT (3D-wPAT) technique is described for brain imaging in behaving rats. 3D-wPAT has three layers of fully functional acoustic transducer arrays. Phantom imaging experiments revealed that the in-plane X-Y spatial resolutions were ~200 μm for each acoustic detection layer. The functional imaging capacity of 3D-wPAT was demonstrated by mapping the cerebral oxygen saturation via multi-wavelength irradiation in behaving hyperoxic rats. In addition, we demonstrated that 3D-wPAT could be used for monitoring sensory stimulus-evoked responses in behaving rats by measuring hemodynamic responses in the primary visual cortex during visual stimulation. Together, these results show the potential of 3D-wPAT for brain study in behaving rodents. Nature Publishing Group 2016-05-05 /pmc/articles/PMC4857106/ /pubmed/27146026 http://dx.doi.org/10.1038/srep25470 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tang, Jianbo
Coleman, Jason E.
Dai, Xianjin
Jiang, Huabei
Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats
title Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats
title_full Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats
title_fullStr Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats
title_full_unstemmed Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats
title_short Wearable 3-D Photoacoustic Tomography for Functional Brain Imaging in Behaving Rats
title_sort wearable 3-d photoacoustic tomography for functional brain imaging in behaving rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857106/
https://www.ncbi.nlm.nih.gov/pubmed/27146026
http://dx.doi.org/10.1038/srep25470
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