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A novel imaging method revealed phosphatidylinositol 3,5-bisphosphate-rich domains in the endosome/lysosome membrane

We developed a new method to observe distribution of phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P(2)] using electron microscopy. In freeze-fracture replicas of quick-frozen samples, PtdIns(3,5)P(2) was labeled specifically using recombinant ATG18 tagged with glutathione S-transferase and 4×FL...

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Detalles Bibliográficos
Autores principales:	Takatori, Sho, Fujimoto, Toyoshi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2016
Materias:	Article Addendum
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857783/ https://www.ncbi.nlm.nih.gov/pubmed/27195064 http://dx.doi.org/10.1080/19420889.2016.1145319

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