Cargando…

A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats

BACKGROUND: Feline diabetes mellitus (DM) shares many pathophysiologic features with human type 2 DM. Human genome‐wide association studies have identified genes associated with obesity and DM, including melanocortin 4 receptor (MC4R), which plays an important role in energy balance and appetite reg...

Descripción completa

Detalles Bibliográficos
Autores principales: Forcada, Y., Holder, A., Church, D.B., Catchpole, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857971/
https://www.ncbi.nlm.nih.gov/pubmed/24372947
http://dx.doi.org/10.1111/jvim.12275
_version_ 1782430723985113088
author Forcada, Y.
Holder, A.
Church, D.B.
Catchpole, B.
author_facet Forcada, Y.
Holder, A.
Church, D.B.
Catchpole, B.
author_sort Forcada, Y.
collection PubMed
description BACKGROUND: Feline diabetes mellitus (DM) shares many pathophysiologic features with human type 2 DM. Human genome‐wide association studies have identified genes associated with obesity and DM, including melanocortin 4 receptor (MC4R), which plays an important role in energy balance and appetite regulation. HYPOTHESIS/OBJECTIVES: To identify single nucleotide polymorphisms (SNPs) in the feline MC4R gene and to determine whether any SNPs are associated with DM or overweight body condition in cats. ANIMALS: Two‐hundred forty domestic shorthaired (DSH) cats were recruited for the study. Of these, 120 diabetics were selected (60 overweight, 60 lean), along with 120 nondiabetic controls (60 overweight and 60 lean). Males and females were equally represented. METHODS: A prospective case‐control study was performed. Genomic DNA was extracted from blood samples and used as template for PCR amplification of the feline MC4R gene. The coding region of the gene was sequenced in 10 cats to identify polymorphisms. Subsequently, genotyping by restriction fragment length polymorphism (RFLP) analysis assessed MC4R:c.92C > T allele and genotype frequencies in each group of cats. RESULTS: No significant differences in MC4R:c.92C>T allele or genotype frequencies were identified between nondiabetic overweight and lean cats. In the overweight diabetic group, 55% were homozygous for the MC4R:c.92C allele, compared to 33% of the lean diabetics and 30% of the nondiabetics. The differences between the overweight diabetic and the nondiabetics were significant (P < .01). CONCLUSIONS AND CLINICAL IMPORTANCE: We identified a polymorphism in the coding sequence of feline MC4R that is associated with DM in overweight DSH cats, similar to the situation in humans.
format Online
Article
Text
id pubmed-4857971
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-48579712016-06-22 A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats Forcada, Y. Holder, A. Church, D.B. Catchpole, B. J Vet Intern Med Standard Articles BACKGROUND: Feline diabetes mellitus (DM) shares many pathophysiologic features with human type 2 DM. Human genome‐wide association studies have identified genes associated with obesity and DM, including melanocortin 4 receptor (MC4R), which plays an important role in energy balance and appetite regulation. HYPOTHESIS/OBJECTIVES: To identify single nucleotide polymorphisms (SNPs) in the feline MC4R gene and to determine whether any SNPs are associated with DM or overweight body condition in cats. ANIMALS: Two‐hundred forty domestic shorthaired (DSH) cats were recruited for the study. Of these, 120 diabetics were selected (60 overweight, 60 lean), along with 120 nondiabetic controls (60 overweight and 60 lean). Males and females were equally represented. METHODS: A prospective case‐control study was performed. Genomic DNA was extracted from blood samples and used as template for PCR amplification of the feline MC4R gene. The coding region of the gene was sequenced in 10 cats to identify polymorphisms. Subsequently, genotyping by restriction fragment length polymorphism (RFLP) analysis assessed MC4R:c.92C > T allele and genotype frequencies in each group of cats. RESULTS: No significant differences in MC4R:c.92C>T allele or genotype frequencies were identified between nondiabetic overweight and lean cats. In the overweight diabetic group, 55% were homozygous for the MC4R:c.92C allele, compared to 33% of the lean diabetics and 30% of the nondiabetics. The differences between the overweight diabetic and the nondiabetics were significant (P < .01). CONCLUSIONS AND CLINICAL IMPORTANCE: We identified a polymorphism in the coding sequence of feline MC4R that is associated with DM in overweight DSH cats, similar to the situation in humans. John Wiley and Sons Inc. 2013-12-26 2014 /pmc/articles/PMC4857971/ /pubmed/24372947 http://dx.doi.org/10.1111/jvim.12275 Text en Copyright © 2013 by the American College of Veterinary Internal Medicine
spellingShingle Standard Articles
Forcada, Y.
Holder, A.
Church, D.B.
Catchpole, B.
A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats
title A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats
title_full A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats
title_fullStr A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats
title_full_unstemmed A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats
title_short A Polymorphism in the Melanocortin 4 Receptor Gene (MC4R:c.92C>T) Is Associated with Diabetes Mellitus in Overweight Domestic Shorthaired Cats
title_sort polymorphism in the melanocortin 4 receptor gene (mc4r:c.92c>t) is associated with diabetes mellitus in overweight domestic shorthaired cats
topic Standard Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857971/
https://www.ncbi.nlm.nih.gov/pubmed/24372947
http://dx.doi.org/10.1111/jvim.12275
work_keys_str_mv AT forcaday apolymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats
AT holdera apolymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats
AT churchdb apolymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats
AT catchpoleb apolymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats
AT forcaday polymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats
AT holdera polymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats
AT churchdb polymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats
AT catchpoleb polymorphisminthemelanocortin4receptorgenemc4rc92ctisassociatedwithdiabetesmellitusinoverweightdomesticshorthairedcats