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Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma
BACKGROUND: Thyroid carcinoma is a common endocrine tumor in the dog. Local invasive growth frequently precludes surgical excision and, in up to 38% of dogs, the tumor has already metastasized by the time of diagnosis. Therefore, it is important to investigate new treatment modalities that may be us...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857974/ https://www.ncbi.nlm.nih.gov/pubmed/24612088 http://dx.doi.org/10.1111/jvim.12330 |
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author | Campos, M. Ducatelle, R. Kooistra, H.S. Rutteman, G. Duchateau, L. Polis, I. Daminet, S. |
author_facet | Campos, M. Ducatelle, R. Kooistra, H.S. Rutteman, G. Duchateau, L. Polis, I. Daminet, S. |
author_sort | Campos, M. |
collection | PubMed |
description | BACKGROUND: Thyroid carcinoma is a common endocrine tumor in the dog. Local invasive growth frequently precludes surgical excision and, in up to 38% of dogs, the tumor has already metastasized by the time of diagnosis. Therefore, it is important to investigate new treatment modalities that may be useful for the large number of dogs with inoperable tumors or metastatic disease. HYPOTHESIS/OBJECTIVES: To investigate the immunohistochemical expression of potential therapeutic targets in canine thyroid tumors. ANIMALS: 74 dogs with thyroid neoplasia. METHODS: Immunohistochemistry was performed for thyroglobulin, calcitonin, vascular endothelial growth factor (VEGF), p53, cycloxygenase‐2 (cox‐2), and P‐glycoprotein (P‐gp). RESULTS: Fifty‐four (73%) tumors were classified as follicular cell thyroid carcinomas (FTCs) and 20 (27%) as medullary thyroid carcinomas (MTCs). Eighty percent of FTCs and all MTCs had a high percentage (76–100%) of neoplastic cells immunopositive for VEGF. Thirteen percent of FTCs and 50% of MTCs expressed cox‐2. Seven percent of FTCs and 70% of MTCs expressed P‐gp. No tumor was immunopositive for p53 expression. Expression of VEGF (P = .034), cox‐2 (P = .013), and P‐gp (P < .001) was significantly higher in MTCs compared to FTCs. CONCLUSIONS AND CLINICAL IMPORTANCE: VEGF is a potential therapeutic target in both FTC and MTC in dogs. Cox‐2 and P‐gp may be useful molecular targets in canine MTC. |
format | Online Article Text |
id | pubmed-4857974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48579742016-06-22 Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma Campos, M. Ducatelle, R. Kooistra, H.S. Rutteman, G. Duchateau, L. Polis, I. Daminet, S. J Vet Intern Med Standard Articles BACKGROUND: Thyroid carcinoma is a common endocrine tumor in the dog. Local invasive growth frequently precludes surgical excision and, in up to 38% of dogs, the tumor has already metastasized by the time of diagnosis. Therefore, it is important to investigate new treatment modalities that may be useful for the large number of dogs with inoperable tumors or metastatic disease. HYPOTHESIS/OBJECTIVES: To investigate the immunohistochemical expression of potential therapeutic targets in canine thyroid tumors. ANIMALS: 74 dogs with thyroid neoplasia. METHODS: Immunohistochemistry was performed for thyroglobulin, calcitonin, vascular endothelial growth factor (VEGF), p53, cycloxygenase‐2 (cox‐2), and P‐glycoprotein (P‐gp). RESULTS: Fifty‐four (73%) tumors were classified as follicular cell thyroid carcinomas (FTCs) and 20 (27%) as medullary thyroid carcinomas (MTCs). Eighty percent of FTCs and all MTCs had a high percentage (76–100%) of neoplastic cells immunopositive for VEGF. Thirteen percent of FTCs and 50% of MTCs expressed cox‐2. Seven percent of FTCs and 70% of MTCs expressed P‐gp. No tumor was immunopositive for p53 expression. Expression of VEGF (P = .034), cox‐2 (P = .013), and P‐gp (P < .001) was significantly higher in MTCs compared to FTCs. CONCLUSIONS AND CLINICAL IMPORTANCE: VEGF is a potential therapeutic target in both FTC and MTC in dogs. Cox‐2 and P‐gp may be useful molecular targets in canine MTC. John Wiley and Sons Inc. 2014-02-24 2014 /pmc/articles/PMC4857974/ /pubmed/24612088 http://dx.doi.org/10.1111/jvim.12330 Text en Copyright © 2014 by the American College of Veterinary Internal Medicine |
spellingShingle | Standard Articles Campos, M. Ducatelle, R. Kooistra, H.S. Rutteman, G. Duchateau, L. Polis, I. Daminet, S. Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma |
title | Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma |
title_full | Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma |
title_fullStr | Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma |
title_full_unstemmed | Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma |
title_short | Immunohistochemical Expression of Potential Therapeutic Targets in Canine Thyroid Carcinoma |
title_sort | immunohistochemical expression of potential therapeutic targets in canine thyroid carcinoma |
topic | Standard Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4857974/ https://www.ncbi.nlm.nih.gov/pubmed/24612088 http://dx.doi.org/10.1111/jvim.12330 |
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