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Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY
Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent study showed that the T6SS is part of the na...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858559/ https://www.ncbi.nlm.nih.gov/pubmed/27117415 http://dx.doi.org/10.1016/j.celrep.2016.03.092 |
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author | Metzger, Lisa C. Stutzmann, Sandrine Scrignari, Tiziana Van der Henst, Charles Matthey, Noémie Blokesch, Melanie |
author_facet | Metzger, Lisa C. Stutzmann, Sandrine Scrignari, Tiziana Van der Henst, Charles Matthey, Noémie Blokesch, Melanie |
author_sort | Metzger, Lisa C. |
collection | PubMed |
description | Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent study showed that the T6SS is part of the natural competence regulon in V. cholerae and is activated by the regulator TfoX. Here, we identify the TfoX homolog TfoY as a second activator of the T6SS. Importantly, despite inducing the same T6SS core machinery, the overall regulons differ significantly for TfoX and TfoY. We show that TfoY does not contribute to competence induction. Instead, TfoY drives the production of T6SS-dependent and T6SS-independent toxins, together with an increased motility phenotype. Hence, we conclude that V. cholerae uses its sole T6SS in response to diverse cues and for distinctive outcomes: either to kill for the prey’s DNA, leading to horizontal gene transfer, or as part of a defensive escape reaction. |
format | Online Article Text |
id | pubmed-4858559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48585592016-05-13 Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY Metzger, Lisa C. Stutzmann, Sandrine Scrignari, Tiziana Van der Henst, Charles Matthey, Noémie Blokesch, Melanie Cell Rep Report Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent study showed that the T6SS is part of the natural competence regulon in V. cholerae and is activated by the regulator TfoX. Here, we identify the TfoX homolog TfoY as a second activator of the T6SS. Importantly, despite inducing the same T6SS core machinery, the overall regulons differ significantly for TfoX and TfoY. We show that TfoY does not contribute to competence induction. Instead, TfoY drives the production of T6SS-dependent and T6SS-independent toxins, together with an increased motility phenotype. Hence, we conclude that V. cholerae uses its sole T6SS in response to diverse cues and for distinctive outcomes: either to kill for the prey’s DNA, leading to horizontal gene transfer, or as part of a defensive escape reaction. Cell Press 2016-04-21 /pmc/articles/PMC4858559/ /pubmed/27117415 http://dx.doi.org/10.1016/j.celrep.2016.03.092 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Report Metzger, Lisa C. Stutzmann, Sandrine Scrignari, Tiziana Van der Henst, Charles Matthey, Noémie Blokesch, Melanie Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY |
title | Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY |
title_full | Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY |
title_fullStr | Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY |
title_full_unstemmed | Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY |
title_short | Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY |
title_sort | independent regulation of type vi secretion in vibrio cholerae by tfox and tfoy |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858559/ https://www.ncbi.nlm.nih.gov/pubmed/27117415 http://dx.doi.org/10.1016/j.celrep.2016.03.092 |
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