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Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY

Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent study showed that the T6SS is part of the na...

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Autores principales: Metzger, Lisa C., Stutzmann, Sandrine, Scrignari, Tiziana, Van der Henst, Charles, Matthey, Noémie, Blokesch, Melanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858559/
https://www.ncbi.nlm.nih.gov/pubmed/27117415
http://dx.doi.org/10.1016/j.celrep.2016.03.092
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author Metzger, Lisa C.
Stutzmann, Sandrine
Scrignari, Tiziana
Van der Henst, Charles
Matthey, Noémie
Blokesch, Melanie
author_facet Metzger, Lisa C.
Stutzmann, Sandrine
Scrignari, Tiziana
Van der Henst, Charles
Matthey, Noémie
Blokesch, Melanie
author_sort Metzger, Lisa C.
collection PubMed
description Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent study showed that the T6SS is part of the natural competence regulon in V. cholerae and is activated by the regulator TfoX. Here, we identify the TfoX homolog TfoY as a second activator of the T6SS. Importantly, despite inducing the same T6SS core machinery, the overall regulons differ significantly for TfoX and TfoY. We show that TfoY does not contribute to competence induction. Instead, TfoY drives the production of T6SS-dependent and T6SS-independent toxins, together with an increased motility phenotype. Hence, we conclude that V. cholerae uses its sole T6SS in response to diverse cues and for distinctive outcomes: either to kill for the prey’s DNA, leading to horizontal gene transfer, or as part of a defensive escape reaction.
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spelling pubmed-48585592016-05-13 Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY Metzger, Lisa C. Stutzmann, Sandrine Scrignari, Tiziana Van der Henst, Charles Matthey, Noémie Blokesch, Melanie Cell Rep Report Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent study showed that the T6SS is part of the natural competence regulon in V. cholerae and is activated by the regulator TfoX. Here, we identify the TfoX homolog TfoY as a second activator of the T6SS. Importantly, despite inducing the same T6SS core machinery, the overall regulons differ significantly for TfoX and TfoY. We show that TfoY does not contribute to competence induction. Instead, TfoY drives the production of T6SS-dependent and T6SS-independent toxins, together with an increased motility phenotype. Hence, we conclude that V. cholerae uses its sole T6SS in response to diverse cues and for distinctive outcomes: either to kill for the prey’s DNA, leading to horizontal gene transfer, or as part of a defensive escape reaction. Cell Press 2016-04-21 /pmc/articles/PMC4858559/ /pubmed/27117415 http://dx.doi.org/10.1016/j.celrep.2016.03.092 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Report
Metzger, Lisa C.
Stutzmann, Sandrine
Scrignari, Tiziana
Van der Henst, Charles
Matthey, Noémie
Blokesch, Melanie
Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY
title Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY
title_full Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY
title_fullStr Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY
title_full_unstemmed Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY
title_short Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY
title_sort independent regulation of type vi secretion in vibrio cholerae by tfox and tfoy
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858559/
https://www.ncbi.nlm.nih.gov/pubmed/27117415
http://dx.doi.org/10.1016/j.celrep.2016.03.092
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