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A Molecular-Level Account of the Antigenic Hantaviral Surface

Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative age...

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Autores principales: Li, Sai, Rissanen, Ilona, Zeltina, Antra, Hepojoki, Jussi, Raghwani, Jayna, Harlos, Karl, Pybus, Oliver G., Huiskonen, Juha T., Bowden, Thomas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858563/
https://www.ncbi.nlm.nih.gov/pubmed/27117403
http://dx.doi.org/10.1016/j.celrep.2016.03.082
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author Li, Sai
Rissanen, Ilona
Zeltina, Antra
Hepojoki, Jussi
Raghwani, Jayna
Harlos, Karl
Pybus, Oliver G.
Huiskonen, Juha T.
Bowden, Thomas A.
author_facet Li, Sai
Rissanen, Ilona
Zeltina, Antra
Hepojoki, Jussi
Raghwani, Jayna
Harlos, Karl
Pybus, Oliver G.
Huiskonen, Juha T.
Bowden, Thomas A.
author_sort Li, Sai
collection PubMed
description Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses.
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spelling pubmed-48585632016-05-13 A Molecular-Level Account of the Antigenic Hantaviral Surface Li, Sai Rissanen, Ilona Zeltina, Antra Hepojoki, Jussi Raghwani, Jayna Harlos, Karl Pybus, Oliver G. Huiskonen, Juha T. Bowden, Thomas A. Cell Rep Report Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses. Cell Press 2016-04-21 /pmc/articles/PMC4858563/ /pubmed/27117403 http://dx.doi.org/10.1016/j.celrep.2016.03.082 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Report
Li, Sai
Rissanen, Ilona
Zeltina, Antra
Hepojoki, Jussi
Raghwani, Jayna
Harlos, Karl
Pybus, Oliver G.
Huiskonen, Juha T.
Bowden, Thomas A.
A Molecular-Level Account of the Antigenic Hantaviral Surface
title A Molecular-Level Account of the Antigenic Hantaviral Surface
title_full A Molecular-Level Account of the Antigenic Hantaviral Surface
title_fullStr A Molecular-Level Account of the Antigenic Hantaviral Surface
title_full_unstemmed A Molecular-Level Account of the Antigenic Hantaviral Surface
title_short A Molecular-Level Account of the Antigenic Hantaviral Surface
title_sort molecular-level account of the antigenic hantaviral surface
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858563/
https://www.ncbi.nlm.nih.gov/pubmed/27117403
http://dx.doi.org/10.1016/j.celrep.2016.03.082
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