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Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats

We previously demonstrated that baicalein could protect against liver ischemia/reperfusion (I/R) injury in mice. The exact mechanism of baicalein remains poorly understood. Autophagy plays an important role in protecting against I/R injury. This study was designed to determine whether baicalein coul...

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Autores principales: Liu, Anding, Huang, Liang, Guo, Enshuang, Li, Renlong, Yang, Jiankun, Li, Anyi, Yang, Yan, Liu, Shenpei, Hu, Jifa, Jiang, Xiaojing, Dirsch, Olaf, Dahmen, Uta, Sun, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858649/
https://www.ncbi.nlm.nih.gov/pubmed/27150843
http://dx.doi.org/10.1038/srep25042
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author Liu, Anding
Huang, Liang
Guo, Enshuang
Li, Renlong
Yang, Jiankun
Li, Anyi
Yang, Yan
Liu, Shenpei
Hu, Jifa
Jiang, Xiaojing
Dirsch, Olaf
Dahmen, Uta
Sun, Jian
author_facet Liu, Anding
Huang, Liang
Guo, Enshuang
Li, Renlong
Yang, Jiankun
Li, Anyi
Yang, Yan
Liu, Shenpei
Hu, Jifa
Jiang, Xiaojing
Dirsch, Olaf
Dahmen, Uta
Sun, Jian
author_sort Liu, Anding
collection PubMed
description We previously demonstrated that baicalein could protect against liver ischemia/reperfusion (I/R) injury in mice. The exact mechanism of baicalein remains poorly understood. Autophagy plays an important role in protecting against I/R injury. This study was designed to determine whether baicalein could protect against liver I/R injury via induction of autophagy in rats. Baicalein was intraperitoneally injected 1 h before warm ischemia. Pretreatment with baicalein prior to I/R insult significantly blunted I/R-induced elevations of serum aminotransferase levels and significantly improved the histological status of livers. Electron microscopy and expression of the autophagic marker LC3B-II suggested induction of autophagy after baicalein treatment. Moreover, inhibition of the baicalein-induced autophagy using 3-methyladenine (3-MA) worsened liver injury. Furthermore, baicalein treatment increased heme oxygenase (HO)-1 expression, and pharmacological inhibition of HO-1 with tin protoporphyrin IX (SnPP) abolished the baicalein-mediated autophagy and the hepatocellular protection. In primary rat hepatocytes, baicalein-induced autophagy also protected hepatocytes from hypoxia/reoxygenation injury in vitro and the beneficial effect was abrogated by 3-MA or Atg7 siRNA, respectively. Suppression of HO-1 activity by SnPP or HO-1 siRNA prevented the baicalein-mediated autophagy and resulted in increased hepatocellular injury. Collectively, these results suggest that baicalein prevents hepatocellular injury via induction of HO-1-mediated autophagy.
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spelling pubmed-48586492016-05-19 Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats Liu, Anding Huang, Liang Guo, Enshuang Li, Renlong Yang, Jiankun Li, Anyi Yang, Yan Liu, Shenpei Hu, Jifa Jiang, Xiaojing Dirsch, Olaf Dahmen, Uta Sun, Jian Sci Rep Article We previously demonstrated that baicalein could protect against liver ischemia/reperfusion (I/R) injury in mice. The exact mechanism of baicalein remains poorly understood. Autophagy plays an important role in protecting against I/R injury. This study was designed to determine whether baicalein could protect against liver I/R injury via induction of autophagy in rats. Baicalein was intraperitoneally injected 1 h before warm ischemia. Pretreatment with baicalein prior to I/R insult significantly blunted I/R-induced elevations of serum aminotransferase levels and significantly improved the histological status of livers. Electron microscopy and expression of the autophagic marker LC3B-II suggested induction of autophagy after baicalein treatment. Moreover, inhibition of the baicalein-induced autophagy using 3-methyladenine (3-MA) worsened liver injury. Furthermore, baicalein treatment increased heme oxygenase (HO)-1 expression, and pharmacological inhibition of HO-1 with tin protoporphyrin IX (SnPP) abolished the baicalein-mediated autophagy and the hepatocellular protection. In primary rat hepatocytes, baicalein-induced autophagy also protected hepatocytes from hypoxia/reoxygenation injury in vitro and the beneficial effect was abrogated by 3-MA or Atg7 siRNA, respectively. Suppression of HO-1 activity by SnPP or HO-1 siRNA prevented the baicalein-mediated autophagy and resulted in increased hepatocellular injury. Collectively, these results suggest that baicalein prevents hepatocellular injury via induction of HO-1-mediated autophagy. Nature Publishing Group 2016-05-06 /pmc/articles/PMC4858649/ /pubmed/27150843 http://dx.doi.org/10.1038/srep25042 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Anding
Huang, Liang
Guo, Enshuang
Li, Renlong
Yang, Jiankun
Li, Anyi
Yang, Yan
Liu, Shenpei
Hu, Jifa
Jiang, Xiaojing
Dirsch, Olaf
Dahmen, Uta
Sun, Jian
Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
title Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
title_full Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
title_fullStr Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
title_full_unstemmed Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
title_short Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
title_sort baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858649/
https://www.ncbi.nlm.nih.gov/pubmed/27150843
http://dx.doi.org/10.1038/srep25042
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