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Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates
BACKGROUND: The agar dilution method is currently considered as the reference method for Mycobacterium marinum drug susceptibility testing (DST). As it is time-consuming, alternative methods, such as the E-test, were evaluated for M.marinum DST, but without success. The SLOMYCO Sensititre(®) panel,...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858841/ https://www.ncbi.nlm.nih.gov/pubmed/27150659 http://dx.doi.org/10.1186/s12941-016-0145-1 |
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author | Chazel, Marion Marchandin, Hélène Keck, Nicolas Terru, Dominique Carrière, Christian Ponsoda, Michael Jacomo, Véronique Panteix, Gilles Bouzinbi, Nicolas Bañuls, Anne-Laure Choisy, Marc Solassol, Jérôme Aubry, Alexandra Godreuil, Sylvain |
author_facet | Chazel, Marion Marchandin, Hélène Keck, Nicolas Terru, Dominique Carrière, Christian Ponsoda, Michael Jacomo, Véronique Panteix, Gilles Bouzinbi, Nicolas Bañuls, Anne-Laure Choisy, Marc Solassol, Jérôme Aubry, Alexandra Godreuil, Sylvain |
author_sort | Chazel, Marion |
collection | PubMed |
description | BACKGROUND: The agar dilution method is currently considered as the reference method for Mycobacterium marinum drug susceptibility testing (DST). As it is time-consuming, alternative methods, such as the E-test, were evaluated for M.marinum DST, but without success. The SLOMYCO Sensititre(®) panel, recently commercialized by TREK Diagnostic Systems (Cleveland, OH), can be used for DST in slow-growing mycobacteria and for antimicrobial agents recommended by the Clinical and Laboratory Standards Institute (CLSI) for M.marinum DST. The main goal of this work was to evaluate the SLOMYCO Sensititre(®) panel method for DST in M.marinum isolates from human patients and fish relative to the reference agar dilution method. METHODS/RESULTS: The reproducibility of the minimum inhibitory concentration (MIC) determination (±1 log(2) dilution) was very good for both the agar dilution method and SLOMYCO Sensititre(®) panel (>90 % agreement). The percentage essential agreement between methods varied, depending on the drug: between 97 and 75 % for ciprofloxacin, moxifloxacin, linezolid, isoniazid, clarithromycin, amikacin, rifabutin and rifampin, 74 % for trimethoprim, 72 % for doxycycline, 70 % for sulfamethoxazole, 59 % for streptomycin, 33 % for ethambutol and only 2.2 % for ethionamide. When the agar dilution and SLOMYCO Sensititre(®) panel results were converted into interpretive criteria, the category agreement was 100 % for amikacin, ciprofloxacin, clarithromycin, moxifloxacin, rifabutin, sulfamethoxazole and trimethoprim, 98 % for ethambutol and 96 % for rifampin and no agreement for doxycycline. CONCLUSIONS: The SLOMYCO Sensititre(®) panel method could provide a potential alternative to the reference agar dilution method, when DST in M.marinum is required, except for doxycycline. |
format | Online Article Text |
id | pubmed-4858841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48588412016-05-07 Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates Chazel, Marion Marchandin, Hélène Keck, Nicolas Terru, Dominique Carrière, Christian Ponsoda, Michael Jacomo, Véronique Panteix, Gilles Bouzinbi, Nicolas Bañuls, Anne-Laure Choisy, Marc Solassol, Jérôme Aubry, Alexandra Godreuil, Sylvain Ann Clin Microbiol Antimicrob Research BACKGROUND: The agar dilution method is currently considered as the reference method for Mycobacterium marinum drug susceptibility testing (DST). As it is time-consuming, alternative methods, such as the E-test, were evaluated for M.marinum DST, but without success. The SLOMYCO Sensititre(®) panel, recently commercialized by TREK Diagnostic Systems (Cleveland, OH), can be used for DST in slow-growing mycobacteria and for antimicrobial agents recommended by the Clinical and Laboratory Standards Institute (CLSI) for M.marinum DST. The main goal of this work was to evaluate the SLOMYCO Sensititre(®) panel method for DST in M.marinum isolates from human patients and fish relative to the reference agar dilution method. METHODS/RESULTS: The reproducibility of the minimum inhibitory concentration (MIC) determination (±1 log(2) dilution) was very good for both the agar dilution method and SLOMYCO Sensititre(®) panel (>90 % agreement). The percentage essential agreement between methods varied, depending on the drug: between 97 and 75 % for ciprofloxacin, moxifloxacin, linezolid, isoniazid, clarithromycin, amikacin, rifabutin and rifampin, 74 % for trimethoprim, 72 % for doxycycline, 70 % for sulfamethoxazole, 59 % for streptomycin, 33 % for ethambutol and only 2.2 % for ethionamide. When the agar dilution and SLOMYCO Sensititre(®) panel results were converted into interpretive criteria, the category agreement was 100 % for amikacin, ciprofloxacin, clarithromycin, moxifloxacin, rifabutin, sulfamethoxazole and trimethoprim, 98 % for ethambutol and 96 % for rifampin and no agreement for doxycycline. CONCLUSIONS: The SLOMYCO Sensititre(®) panel method could provide a potential alternative to the reference agar dilution method, when DST in M.marinum is required, except for doxycycline. BioMed Central 2016-05-06 /pmc/articles/PMC4858841/ /pubmed/27150659 http://dx.doi.org/10.1186/s12941-016-0145-1 Text en © Chazel et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Chazel, Marion Marchandin, Hélène Keck, Nicolas Terru, Dominique Carrière, Christian Ponsoda, Michael Jacomo, Véronique Panteix, Gilles Bouzinbi, Nicolas Bañuls, Anne-Laure Choisy, Marc Solassol, Jérôme Aubry, Alexandra Godreuil, Sylvain Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates |
title | Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates |
title_full | Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates |
title_fullStr | Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates |
title_full_unstemmed | Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates |
title_short | Evaluation of the SLOMYCO Sensititre(®) panel for testing the antimicrobial susceptibility of Mycobacterium marinum isolates |
title_sort | evaluation of the slomyco sensititre(®) panel for testing the antimicrobial susceptibility of mycobacterium marinum isolates |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858841/ https://www.ncbi.nlm.nih.gov/pubmed/27150659 http://dx.doi.org/10.1186/s12941-016-0145-1 |
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