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Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device
Cell invasion is the first step of cancer metastasis that is the primary cause of death for cancer patients and defined as cell movement through extracellular matrix (ECM). Investigation of the correlation between cell invasive and extracellular stimulation is critical for the inhabitation of metast...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858876/ https://www.ncbi.nlm.nih.gov/pubmed/27150137 http://dx.doi.org/10.1038/srep25557 |
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author | Lei, Kin Fong Tseng, Hsueh-Peng Lee, Chia-Yi Tsang, Ngan-Ming |
author_facet | Lei, Kin Fong Tseng, Hsueh-Peng Lee, Chia-Yi Tsang, Ngan-Ming |
author_sort | Lei, Kin Fong |
collection | PubMed |
description | Cell invasion is the first step of cancer metastasis that is the primary cause of death for cancer patients and defined as cell movement through extracellular matrix (ECM). Investigation of the correlation between cell invasive and extracellular stimulation is critical for the inhabitation of metastatic dissemination. Conventional cell invasion assay is based on Boyden chamber assay, which has a number of limitations. In this work, a microfluidic device incorporating with impedance measurement technique was developed for quantitative investigation of cell invasion process. The device consisted of 2 reservoirs connecting with a microchannel filled with hydrogel. Malignant cells invaded along the microchannel and impedance measurement was concurrently conducted by measuring across electrodes located at the bottom of the microchannel. Therefore, cell invasion process could be monitored in real-time and non-invasive manner. Also, cell invasion rate was then calculated to study the correlation between cell invasion and extracellular stimulation, i.e., IL-6 cytokine. Results showed that cell invasion rate was directly proportional to the IL-6 concentration. The microfluidic device provides a reliable and convenient platform for cell-based assays to facilitate more quantitative assessments in cancer research. |
format | Online Article Text |
id | pubmed-4858876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48588762016-05-20 Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device Lei, Kin Fong Tseng, Hsueh-Peng Lee, Chia-Yi Tsang, Ngan-Ming Sci Rep Article Cell invasion is the first step of cancer metastasis that is the primary cause of death for cancer patients and defined as cell movement through extracellular matrix (ECM). Investigation of the correlation between cell invasive and extracellular stimulation is critical for the inhabitation of metastatic dissemination. Conventional cell invasion assay is based on Boyden chamber assay, which has a number of limitations. In this work, a microfluidic device incorporating with impedance measurement technique was developed for quantitative investigation of cell invasion process. The device consisted of 2 reservoirs connecting with a microchannel filled with hydrogel. Malignant cells invaded along the microchannel and impedance measurement was concurrently conducted by measuring across electrodes located at the bottom of the microchannel. Therefore, cell invasion process could be monitored in real-time and non-invasive manner. Also, cell invasion rate was then calculated to study the correlation between cell invasion and extracellular stimulation, i.e., IL-6 cytokine. Results showed that cell invasion rate was directly proportional to the IL-6 concentration. The microfluidic device provides a reliable and convenient platform for cell-based assays to facilitate more quantitative assessments in cancer research. Nature Publishing Group 2016-05-06 /pmc/articles/PMC4858876/ /pubmed/27150137 http://dx.doi.org/10.1038/srep25557 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Lei, Kin Fong Tseng, Hsueh-Peng Lee, Chia-Yi Tsang, Ngan-Ming Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device |
title | Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device |
title_full | Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device |
title_fullStr | Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device |
title_full_unstemmed | Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device |
title_short | Quantitative Study of Cell Invasion Process under Extracellular Stimulation of Cytokine in a Microfluidic Device |
title_sort | quantitative study of cell invasion process under extracellular stimulation of cytokine in a microfluidic device |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4858876/ https://www.ncbi.nlm.nih.gov/pubmed/27150137 http://dx.doi.org/10.1038/srep25557 |
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