Quantitating Fluorescence Intensity From Fluorophores: Practical Use of MESF Values

The present work uses fluorescein as the model fluorophore and points out critical steps in the use of MESF (Molecules of Equivalent Soluble Fluorophores) values for quantitative flow cytometric measurements. It has been found that emission spectrum matching between a reference solution and an analy...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Lili, Gaigalas, Adolfas K., Abbasi, Fatima, Marti, Gerald E., Vogt, Robert F., Schwartz, Abe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology 2002
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859266/
https://www.ncbi.nlm.nih.gov/pubmed/27446735
http://dx.doi.org/10.6028/jres.107.027

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859266/
https://www.ncbi.nlm.nih.gov/pubmed/27446735
http://dx.doi.org/10.6028/jres.107.027

Ejemplares similares