Cargando…

Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought

We have examined the biochemical responses of two sorghum cultivars of differing drought tolerance, Samsorg 17 (more drought tolerant) and Samsorg 40 (less drought tolerant), to sustained drought. Plants were exposed to different degrees of drought and then maintained at that level for five days. Re...

Descripción completa

Detalles Bibliográficos
Autores principales: Ogbaga, Chukwuma C., Stepien, Piotr, Dyson, Beth C., Rattray, Nicholas J. W., Ellis, David I., Goodacre, Royston, Johnson, Giles N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859509/
https://www.ncbi.nlm.nih.gov/pubmed/27153323
http://dx.doi.org/10.1371/journal.pone.0154423
_version_ 1782430976061734912
author Ogbaga, Chukwuma C.
Stepien, Piotr
Dyson, Beth C.
Rattray, Nicholas J. W.
Ellis, David I.
Goodacre, Royston
Johnson, Giles N.
author_facet Ogbaga, Chukwuma C.
Stepien, Piotr
Dyson, Beth C.
Rattray, Nicholas J. W.
Ellis, David I.
Goodacre, Royston
Johnson, Giles N.
author_sort Ogbaga, Chukwuma C.
collection PubMed
description We have examined the biochemical responses of two sorghum cultivars of differing drought tolerance, Samsorg 17 (more drought tolerant) and Samsorg 40 (less drought tolerant), to sustained drought. Plants were exposed to different degrees of drought and then maintained at that level for five days. Responses were examined in terms of metabolic changes and the expression of drought induced proteins—Heat Shock Proteins (HSPs) and dehydrins (DHNs). Generalised phenotypic changes were studied using Fourier transform infrared (FT-IR) Spectroscopy and non-targeted Gas Chromatography Mass Spectrometry (GC-MS) was employed to detect changes in metabolites, while changes in protein expression were examined using Western blot analysis. Different response profiles of metabolites, HSPs and DHNs were observed in the two cultivars. Metabolic changes involved variation in amino acids, polysaccharides and their derivatives. A total of 188 compounds, with 142 known metabolites and 46 unknown small molecules, were detected in the two sorghum varieties. Under water deficit conditions, Samsorg 17 accumulated sugars and sugar alcohols, while in Samsorg 40 amino acids increased in concentration. This study suggest that the two Sorghum varieties adopt distinct approaches in response to drought, with Samsorg 17 being better able to maintain leaf function under severe drought conditions.
format Online
Article
Text
id pubmed-4859509
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-48595092016-05-13 Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought Ogbaga, Chukwuma C. Stepien, Piotr Dyson, Beth C. Rattray, Nicholas J. W. Ellis, David I. Goodacre, Royston Johnson, Giles N. PLoS One Research Article We have examined the biochemical responses of two sorghum cultivars of differing drought tolerance, Samsorg 17 (more drought tolerant) and Samsorg 40 (less drought tolerant), to sustained drought. Plants were exposed to different degrees of drought and then maintained at that level for five days. Responses were examined in terms of metabolic changes and the expression of drought induced proteins—Heat Shock Proteins (HSPs) and dehydrins (DHNs). Generalised phenotypic changes were studied using Fourier transform infrared (FT-IR) Spectroscopy and non-targeted Gas Chromatography Mass Spectrometry (GC-MS) was employed to detect changes in metabolites, while changes in protein expression were examined using Western blot analysis. Different response profiles of metabolites, HSPs and DHNs were observed in the two cultivars. Metabolic changes involved variation in amino acids, polysaccharides and their derivatives. A total of 188 compounds, with 142 known metabolites and 46 unknown small molecules, were detected in the two sorghum varieties. Under water deficit conditions, Samsorg 17 accumulated sugars and sugar alcohols, while in Samsorg 40 amino acids increased in concentration. This study suggest that the two Sorghum varieties adopt distinct approaches in response to drought, with Samsorg 17 being better able to maintain leaf function under severe drought conditions. Public Library of Science 2016-05-06 /pmc/articles/PMC4859509/ /pubmed/27153323 http://dx.doi.org/10.1371/journal.pone.0154423 Text en © 2016 Ogbaga et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ogbaga, Chukwuma C.
Stepien, Piotr
Dyson, Beth C.
Rattray, Nicholas J. W.
Ellis, David I.
Goodacre, Royston
Johnson, Giles N.
Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought
title Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought
title_full Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought
title_fullStr Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought
title_full_unstemmed Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought
title_short Biochemical Analyses of Sorghum Varieties Reveal Differential Responses to Drought
title_sort biochemical analyses of sorghum varieties reveal differential responses to drought
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859509/
https://www.ncbi.nlm.nih.gov/pubmed/27153323
http://dx.doi.org/10.1371/journal.pone.0154423
work_keys_str_mv AT ogbagachukwumac biochemicalanalysesofsorghumvarietiesrevealdifferentialresponsestodrought
AT stepienpiotr biochemicalanalysesofsorghumvarietiesrevealdifferentialresponsestodrought
AT dysonbethc biochemicalanalysesofsorghumvarietiesrevealdifferentialresponsestodrought
AT rattraynicholasjw biochemicalanalysesofsorghumvarietiesrevealdifferentialresponsestodrought
AT ellisdavidi biochemicalanalysesofsorghumvarietiesrevealdifferentialresponsestodrought
AT goodacreroyston biochemicalanalysesofsorghumvarietiesrevealdifferentialresponsestodrought
AT johnsongilesn biochemicalanalysesofsorghumvarietiesrevealdifferentialresponsestodrought