The RNA Helicase eIF4A Is Required for Sapovirus Translation

The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5′ untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is r...

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Detalles Bibliográficos
Autores principales: Hosmillo, Myra, Sweeney, Trevor R., Chaudhry, Yasmin, Leen, Eoin, Curry, Stephen, Goodfellow, Ian, Cho, Kyoung-Oh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Virus-Cell Interactions
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859703/
https://www.ncbi.nlm.nih.gov/pubmed/26937032
http://dx.doi.org/10.1128/JVI.03174-15
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author Hosmillo, Myra
Sweeney, Trevor R.
Chaudhry, Yasmin
Leen, Eoin
Curry, Stephen
Goodfellow, Ian
Cho, Kyoung-Oh
author_facet Hosmillo, Myra
Sweeney, Trevor R.
Chaudhry, Yasmin
Leen, Eoin
Curry, Stephen
Goodfellow, Ian
Cho, Kyoung-Oh
author_sort Hosmillo, Myra
collection PubMed
description The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5′ untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5′ UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.
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spelling pubmed-48597032016-06-06 The RNA Helicase eIF4A Is Required for Sapovirus Translation Hosmillo, Myra Sweeney, Trevor R. Chaudhry, Yasmin Leen, Eoin Curry, Stephen Goodfellow, Ian Cho, Kyoung-Oh J Virol Virus-Cell Interactions The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5′ untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5′ UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation. American Society for Microbiology 2016-04-29 /pmc/articles/PMC4859703/ /pubmed/26937032 http://dx.doi.org/10.1128/JVI.03174-15 Text en Copyright © 2016 Hosmillo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Virus-Cell Interactions
Hosmillo, Myra
Sweeney, Trevor R.
Chaudhry, Yasmin
Leen, Eoin
Curry, Stephen
Goodfellow, Ian
Cho, Kyoung-Oh
The RNA Helicase eIF4A Is Required for Sapovirus Translation
title The RNA Helicase eIF4A Is Required for Sapovirus Translation
title_full The RNA Helicase eIF4A Is Required for Sapovirus Translation
title_fullStr The RNA Helicase eIF4A Is Required for Sapovirus Translation
title_full_unstemmed The RNA Helicase eIF4A Is Required for Sapovirus Translation
title_short The RNA Helicase eIF4A Is Required for Sapovirus Translation
title_sort rna helicase eif4a is required for sapovirus translation
topic Virus-Cell Interactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859703/
https://www.ncbi.nlm.nih.gov/pubmed/26937032
http://dx.doi.org/10.1128/JVI.03174-15
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