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In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm

Objective: This study was conducted to investigate the effects of low-level laser (LLL) doses on human red blood cell volume. The effects of exposure to a diode pump solid state (DPSS) (λ = 405 nm) laser were observed. Background data: The response of human blood to LLL irradiation gives important i...

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Autores principales: Musawi, Mustafa S. Al, Jafar, Mohamad Suhaimi, Al-Gailani, Bassam T., Ahmed, Naser Mahmoud, Suhaimi, Fatanah Mohamad, Suardi, Nursakinah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860659/
https://www.ncbi.nlm.nih.gov/pubmed/26966989
http://dx.doi.org/10.1089/pho.2015.4043
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author Musawi, Mustafa S. Al
Jafar, Mohamad Suhaimi
Al-Gailani, Bassam T.
Ahmed, Naser Mahmoud
Suhaimi, Fatanah Mohamad
Suardi, Nursakinah
author_facet Musawi, Mustafa S. Al
Jafar, Mohamad Suhaimi
Al-Gailani, Bassam T.
Ahmed, Naser Mahmoud
Suhaimi, Fatanah Mohamad
Suardi, Nursakinah
author_sort Musawi, Mustafa S. Al
collection PubMed
description Objective: This study was conducted to investigate the effects of low-level laser (LLL) doses on human red blood cell volume. The effects of exposure to a diode pump solid state (DPSS) (λ = 405 nm) laser were observed. Background data: The response of human blood to LLL irradiation gives important information about the mechanism of interaction of laser light with living organisms. Materials and methods Blood samples were collected into ethylenediaminetetraacetic acid (EDTA)-containing tubes, and each sample was divided into two equal aliquots, one to serve as control and the other for irradiation. The aliquot was subjected to laser irradiation for 20, 30, 40, or 50 min at a fixed power density of 0.03 W/cm(2). Mean cell volume (MCV) and red blood cell (RBC) counts were measured immediately after irradiation using a computerized hemtoanalyzer. Results: Significant decrease in RBC volume (p < 0.05, p < 0.0001, p < 0.0001, and p < 0.05, respectively) was induced with variation in laser doses.The highest response was observed with an exposure time of 40 min. This result was reproduced in RBCs suspended in a buffered NaCl solution. In contrast to this finding, laser-induced RBC volume change was completely abolished by suspending RBCs in a solution containing a higher concentration of EDTA. Conclusions: It was suggested that LLL can reduce RBC volume possibly because of the increased free intracellular Ca(+2) concentrations, which activate Ca(+2)-dependent K(+) channels with consequent K(+) ion efflux and cell shrinkage.
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spelling pubmed-48606592016-05-17 In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm Musawi, Mustafa S. Al Jafar, Mohamad Suhaimi Al-Gailani, Bassam T. Ahmed, Naser Mahmoud Suhaimi, Fatanah Mohamad Suardi, Nursakinah Photomed Laser Surg Original Research Objective: This study was conducted to investigate the effects of low-level laser (LLL) doses on human red blood cell volume. The effects of exposure to a diode pump solid state (DPSS) (λ = 405 nm) laser were observed. Background data: The response of human blood to LLL irradiation gives important information about the mechanism of interaction of laser light with living organisms. Materials and methods Blood samples were collected into ethylenediaminetetraacetic acid (EDTA)-containing tubes, and each sample was divided into two equal aliquots, one to serve as control and the other for irradiation. The aliquot was subjected to laser irradiation for 20, 30, 40, or 50 min at a fixed power density of 0.03 W/cm(2). Mean cell volume (MCV) and red blood cell (RBC) counts were measured immediately after irradiation using a computerized hemtoanalyzer. Results: Significant decrease in RBC volume (p < 0.05, p < 0.0001, p < 0.0001, and p < 0.05, respectively) was induced with variation in laser doses.The highest response was observed with an exposure time of 40 min. This result was reproduced in RBCs suspended in a buffered NaCl solution. In contrast to this finding, laser-induced RBC volume change was completely abolished by suspending RBCs in a solution containing a higher concentration of EDTA. Conclusions: It was suggested that LLL can reduce RBC volume possibly because of the increased free intracellular Ca(+2) concentrations, which activate Ca(+2)-dependent K(+) channels with consequent K(+) ion efflux and cell shrinkage. Mary Ann Liebert, Inc. 2016-05-01 /pmc/articles/PMC4860659/ /pubmed/26966989 http://dx.doi.org/10.1089/pho.2015.4043 Text en © Mustafa S. Al Musawi, et al., 2016; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Original Research
Musawi, Mustafa S. Al
Jafar, Mohamad Suhaimi
Al-Gailani, Bassam T.
Ahmed, Naser Mahmoud
Suhaimi, Fatanah Mohamad
Suardi, Nursakinah
In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm
title In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm
title_full In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm
title_fullStr In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm
title_full_unstemmed In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm
title_short In Vitro Mean Red Blood Cell Volume Change Induced by Diode Pump Solid State Low-Level Laser of 405 nm
title_sort in vitro mean red blood cell volume change induced by diode pump solid state low-level laser of 405 nm
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860659/
https://www.ncbi.nlm.nih.gov/pubmed/26966989
http://dx.doi.org/10.1089/pho.2015.4043
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