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Autologous iPSC-derived dopamine neuron transplantation in a nonhuman primate Parkinson’s disease model
Autologous dopamine (DA) neurons are a new cell source for replacement therapy of Parkinson’s disease (PD). In this study, we tested the safety and efficacy of autologous induced pluripotent stem cell (iPSC)-derived DA cells for treatment of a cynomolgus monkey PD model. Monkey bone marrow mesenchym...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860772/ https://www.ncbi.nlm.nih.gov/pubmed/27462412 http://dx.doi.org/10.1038/celldisc.2015.12 |
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author | Wang, Shuyan Zou, Chunlin Fu, Linlin Wang, Bin An, Jing Song, Gongru Wu, Jianyu Tang, Xihe Li, Mo Zhang, Jian Yue, Feng Zheng, Chengyun Chan, Piu Zhang, Y Alex Chen, Zhiguo |
author_facet | Wang, Shuyan Zou, Chunlin Fu, Linlin Wang, Bin An, Jing Song, Gongru Wu, Jianyu Tang, Xihe Li, Mo Zhang, Jian Yue, Feng Zheng, Chengyun Chan, Piu Zhang, Y Alex Chen, Zhiguo |
author_sort | Wang, Shuyan |
collection | PubMed |
description | Autologous dopamine (DA) neurons are a new cell source for replacement therapy of Parkinson’s disease (PD). In this study, we tested the safety and efficacy of autologous induced pluripotent stem cell (iPSC)-derived DA cells for treatment of a cynomolgus monkey PD model. Monkey bone marrow mesenchymal cells were isolated and induced to iPSCs, followed by differentiation into DA cells using a method with high efficiency. Autologous DA cells were introduced into the brain of a cynomolgus monkey PD model without immunosuppression; three PD monkeys that had received no grafts served as controls. The PD monkey that had received autologous grafts experienced behavioral improvement compared with that of controls. Histological analysis revealed no overgrowth of grafts and a significant number of surviving A9 region-specific graft-derived DA neurons. The study provided a proof-of-principle to employ iPSC-derived autologous DA cells for PD treatment using a nonhuman primate PD model. |
format | Online Article Text |
id | pubmed-4860772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48607722016-07-26 Autologous iPSC-derived dopamine neuron transplantation in a nonhuman primate Parkinson’s disease model Wang, Shuyan Zou, Chunlin Fu, Linlin Wang, Bin An, Jing Song, Gongru Wu, Jianyu Tang, Xihe Li, Mo Zhang, Jian Yue, Feng Zheng, Chengyun Chan, Piu Zhang, Y Alex Chen, Zhiguo Cell Discov Article Autologous dopamine (DA) neurons are a new cell source for replacement therapy of Parkinson’s disease (PD). In this study, we tested the safety and efficacy of autologous induced pluripotent stem cell (iPSC)-derived DA cells for treatment of a cynomolgus monkey PD model. Monkey bone marrow mesenchymal cells were isolated and induced to iPSCs, followed by differentiation into DA cells using a method with high efficiency. Autologous DA cells were introduced into the brain of a cynomolgus monkey PD model without immunosuppression; three PD monkeys that had received no grafts served as controls. The PD monkey that had received autologous grafts experienced behavioral improvement compared with that of controls. Histological analysis revealed no overgrowth of grafts and a significant number of surviving A9 region-specific graft-derived DA neurons. The study provided a proof-of-principle to employ iPSC-derived autologous DA cells for PD treatment using a nonhuman primate PD model. Nature Publishing Group 2015-05-26 /pmc/articles/PMC4860772/ /pubmed/27462412 http://dx.doi.org/10.1038/celldisc.2015.12 Text en Copyright © 2015 SIBS, CAS http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, Shuyan Zou, Chunlin Fu, Linlin Wang, Bin An, Jing Song, Gongru Wu, Jianyu Tang, Xihe Li, Mo Zhang, Jian Yue, Feng Zheng, Chengyun Chan, Piu Zhang, Y Alex Chen, Zhiguo Autologous iPSC-derived dopamine neuron transplantation in a nonhuman primate Parkinson’s disease model |
title | Autologous iPSC-derived dopamine neuron transplantation in a nonhuman
primate Parkinson’s disease model |
title_full | Autologous iPSC-derived dopamine neuron transplantation in a nonhuman
primate Parkinson’s disease model |
title_fullStr | Autologous iPSC-derived dopamine neuron transplantation in a nonhuman
primate Parkinson’s disease model |
title_full_unstemmed | Autologous iPSC-derived dopamine neuron transplantation in a nonhuman
primate Parkinson’s disease model |
title_short | Autologous iPSC-derived dopamine neuron transplantation in a nonhuman
primate Parkinson’s disease model |
title_sort | autologous ipsc-derived dopamine neuron transplantation in a nonhuman
primate parkinson’s disease model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860772/ https://www.ncbi.nlm.nih.gov/pubmed/27462412 http://dx.doi.org/10.1038/celldisc.2015.12 |
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