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Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration
The Hippo signaling pathway regulates tissue growth and organ size through controlling cell growth, proliferation and apoptosis. During these processes, the coactivator Yorkie partners with the transcription factor Scalloped to mediate Hippo pathway-regulated cellular functions. Here, we demonstrate...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860958/ https://www.ncbi.nlm.nih.gov/pubmed/27462453 http://dx.doi.org/10.1038/celldisc.2016.6 |
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author | Wang, Chao Yin, Meng-Xin Wu, Wei Dong, Liang Wang, Shimin Lu, Yi Xu, Jinjin Wu, Wenqing Li, Sheng Zhao, Yun Zhang, Lei |
author_facet | Wang, Chao Yin, Meng-Xin Wu, Wei Dong, Liang Wang, Shimin Lu, Yi Xu, Jinjin Wu, Wenqing Li, Sheng Zhao, Yun Zhang, Lei |
author_sort | Wang, Chao |
collection | PubMed |
description | The Hippo signaling pathway regulates tissue growth and organ size through controlling cell growth, proliferation and apoptosis. During these processes, the coactivator Yorkie partners with the transcription factor Scalloped to mediate Hippo pathway-regulated cellular functions. Here, we demonstrate that Taiman facilitates the activity of Yorkie. First, Taiman overexpression upregulates Hippo pathway-responsive genes and induces tissue overgrowth. Second, the loss of tai downregulates the expression of Hippo pathway target genes and reduces organ size as well as tissue overgrowth caused by Yorkie overexpression. Furthermore, we provide evidence that Taiman binds to Yorkie and facilitates the activity of Yorkie-Scalloped to activate the transcription of several Hippo pathway target genes. Moreover, we found that the C-terminus of Taiman is indispensable for the function of Taiman in Hippo signaling. Finally, we demonstrate that Taiman is also required in intestinal stem cell proliferation. Our findings suggest Taiman is an essential coactivator of Yorkie. |
format | Online Article Text |
id | pubmed-4860958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48609582016-07-26 Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration Wang, Chao Yin, Meng-Xin Wu, Wei Dong, Liang Wang, Shimin Lu, Yi Xu, Jinjin Wu, Wenqing Li, Sheng Zhao, Yun Zhang, Lei Cell Discov Article The Hippo signaling pathway regulates tissue growth and organ size through controlling cell growth, proliferation and apoptosis. During these processes, the coactivator Yorkie partners with the transcription factor Scalloped to mediate Hippo pathway-regulated cellular functions. Here, we demonstrate that Taiman facilitates the activity of Yorkie. First, Taiman overexpression upregulates Hippo pathway-responsive genes and induces tissue overgrowth. Second, the loss of tai downregulates the expression of Hippo pathway target genes and reduces organ size as well as tissue overgrowth caused by Yorkie overexpression. Furthermore, we provide evidence that Taiman binds to Yorkie and facilitates the activity of Yorkie-Scalloped to activate the transcription of several Hippo pathway target genes. Moreover, we found that the C-terminus of Taiman is indispensable for the function of Taiman in Hippo signaling. Finally, we demonstrate that Taiman is also required in intestinal stem cell proliferation. Our findings suggest Taiman is an essential coactivator of Yorkie. Nature Publishing Group 2016-03-22 /pmc/articles/PMC4860958/ /pubmed/27462453 http://dx.doi.org/10.1038/celldisc.2016.6 Text en Copyright © 2016 SIBS, CAS http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, Chao Yin, Meng-Xin Wu, Wei Dong, Liang Wang, Shimin Lu, Yi Xu, Jinjin Wu, Wenqing Li, Sheng Zhao, Yun Zhang, Lei Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration |
title | Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration |
title_full | Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration |
title_fullStr | Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration |
title_full_unstemmed | Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration |
title_short | Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration |
title_sort | taiman acts as a coactivator of yorkie in the hippo pathway to promote tissue growth and intestinal regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4860958/ https://www.ncbi.nlm.nih.gov/pubmed/27462453 http://dx.doi.org/10.1038/celldisc.2016.6 |
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