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Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance
Potassium (K(+)) is the most important cationic nutrient for all living organisms and has roles in most aspects of plant physiology. To assess the impact of one of the main K(+) uptake components, the K(+) inward rectifying channel AKT1, we characterized both loss of function and overexpression of O...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861017/ https://www.ncbi.nlm.nih.gov/pubmed/26969743 http://dx.doi.org/10.1093/jxb/erw103 |
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author | Ahmad, Izhar Mian, Afaq Maathuis, Frans J. M. |
author_facet | Ahmad, Izhar Mian, Afaq Maathuis, Frans J. M. |
author_sort | Ahmad, Izhar |
collection | PubMed |
description | Potassium (K(+)) is the most important cationic nutrient for all living organisms and has roles in most aspects of plant physiology. To assess the impact of one of the main K(+) uptake components, the K(+) inward rectifying channel AKT1, we characterized both loss of function and overexpression of OsAKT1 in rice. In many conditions, AKT1 expression correlated with K(+) uptake and tissue K(+) levels. No salinity-related growth phenotype was observed for either loss or gain of function mutants. However, a correlation between AKT1 expression and root Na(+) when the external Na/K ratio was high suggests that there may be a role for AKT1 in Na(+) uptake in such conditions. In contrast to findings with Arabidopsis thaliana, we did not detect any change in growth of AKT1 loss of function mutants in the presence of NH(4) (+). Nevertheless, NH(4) (+)-dependent inhibition was detected during K(+) uptake assays in loss of function and wild type plants, depending on pre-growth conditions. The most prominent result of OsAKT1 overexpression was a reduction in sensitivity to osmotic/drought stress in transgenic plants: the data suggest that AKT1 overexpression improved rice osmotic and drought stress tolerance by increasing tissue levels of K(+), especially in the root. |
format | Online Article Text |
id | pubmed-4861017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48610172016-05-10 Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance Ahmad, Izhar Mian, Afaq Maathuis, Frans J. M. J Exp Bot Research Paper Potassium (K(+)) is the most important cationic nutrient for all living organisms and has roles in most aspects of plant physiology. To assess the impact of one of the main K(+) uptake components, the K(+) inward rectifying channel AKT1, we characterized both loss of function and overexpression of OsAKT1 in rice. In many conditions, AKT1 expression correlated with K(+) uptake and tissue K(+) levels. No salinity-related growth phenotype was observed for either loss or gain of function mutants. However, a correlation between AKT1 expression and root Na(+) when the external Na/K ratio was high suggests that there may be a role for AKT1 in Na(+) uptake in such conditions. In contrast to findings with Arabidopsis thaliana, we did not detect any change in growth of AKT1 loss of function mutants in the presence of NH(4) (+). Nevertheless, NH(4) (+)-dependent inhibition was detected during K(+) uptake assays in loss of function and wild type plants, depending on pre-growth conditions. The most prominent result of OsAKT1 overexpression was a reduction in sensitivity to osmotic/drought stress in transgenic plants: the data suggest that AKT1 overexpression improved rice osmotic and drought stress tolerance by increasing tissue levels of K(+), especially in the root. Oxford University Press 2016-04 2016-03-11 /pmc/articles/PMC4861017/ /pubmed/26969743 http://dx.doi.org/10.1093/jxb/erw103 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Ahmad, Izhar Mian, Afaq Maathuis, Frans J. M. Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance |
title | Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance |
title_full | Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance |
title_fullStr | Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance |
title_full_unstemmed | Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance |
title_short | Overexpression of the rice AKT1 potassium channel affects potassium nutrition and rice drought tolerance |
title_sort | overexpression of the rice akt1 potassium channel affects potassium nutrition and rice drought tolerance |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861017/ https://www.ncbi.nlm.nih.gov/pubmed/26969743 http://dx.doi.org/10.1093/jxb/erw103 |
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