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Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation

The culm (particularly the flag leaf) and the ear are believed to play a major role in providing assimilates for grain filling in wheat. However, the results obtained in the past varied depending on the methodology applied. Three different methodologies were compared that aimed to assess the relativ...

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Autores principales: Sanchez-Bragado, Rut, Molero, Gemma, Reynolds, Matthew P., Araus, Jose Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861024/
https://www.ncbi.nlm.nih.gov/pubmed/27012283
http://dx.doi.org/10.1093/jxb/erw116
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author Sanchez-Bragado, Rut
Molero, Gemma
Reynolds, Matthew P.
Araus, Jose Luis
author_facet Sanchez-Bragado, Rut
Molero, Gemma
Reynolds, Matthew P.
Araus, Jose Luis
author_sort Sanchez-Bragado, Rut
collection PubMed
description The culm (particularly the flag leaf) and the ear are believed to play a major role in providing assimilates for grain filling in wheat. However, the results obtained in the past varied depending on the methodology applied. Three different methodologies were compared that aimed to assess the relative contribution of the culm (photosynthetic organs below the ear) and the ear to grain filling. The first two consisted of applications of photosynthesis inhibition treatments, including the use of the herbicide DCMU and organ shading. The third was a non-intrusive method that compared the carbon isotope composition (δ(13)C) of mature kernels with the δ(13)C of the water-soluble fraction of the peduncle, awns and glumes. Several advanced CIMMYT lines were tested under good agronomic conditions. The δ(13)C approach assigned a higher photosynthetic contribution to the ear than to the culm. However, some methodological considerations should be taken into account when applying the δ(13)C approach, particularly the sampling method used, in order to prevent post-harvest respiration. The shading approach assigned a similar contribution to the ear as to the culm. The DCMU approach assigned a greater role to the culm but herbicide application to the culm affected the ear, thus biasing the final grain weight. Moreover DCMU and shading approaches may cause compensatory effects which overestimated the contribution of unaffected organs. This study may help to develop precise phenotyping tools to identify physiological traits such as ear photosynthesis that could contribute towards increasing grain yield.
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spelling pubmed-48610242016-05-10 Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation Sanchez-Bragado, Rut Molero, Gemma Reynolds, Matthew P. Araus, Jose Luis J Exp Bot Research Paper The culm (particularly the flag leaf) and the ear are believed to play a major role in providing assimilates for grain filling in wheat. However, the results obtained in the past varied depending on the methodology applied. Three different methodologies were compared that aimed to assess the relative contribution of the culm (photosynthetic organs below the ear) and the ear to grain filling. The first two consisted of applications of photosynthesis inhibition treatments, including the use of the herbicide DCMU and organ shading. The third was a non-intrusive method that compared the carbon isotope composition (δ(13)C) of mature kernels with the δ(13)C of the water-soluble fraction of the peduncle, awns and glumes. Several advanced CIMMYT lines were tested under good agronomic conditions. The δ(13)C approach assigned a higher photosynthetic contribution to the ear than to the culm. However, some methodological considerations should be taken into account when applying the δ(13)C approach, particularly the sampling method used, in order to prevent post-harvest respiration. The shading approach assigned a similar contribution to the ear as to the culm. The DCMU approach assigned a greater role to the culm but herbicide application to the culm affected the ear, thus biasing the final grain weight. Moreover DCMU and shading approaches may cause compensatory effects which overestimated the contribution of unaffected organs. This study may help to develop precise phenotyping tools to identify physiological traits such as ear photosynthesis that could contribute towards increasing grain yield. Oxford University Press 2016-04 2016-03-24 /pmc/articles/PMC4861024/ /pubmed/27012283 http://dx.doi.org/10.1093/jxb/erw116 Text en © The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Sanchez-Bragado, Rut
Molero, Gemma
Reynolds, Matthew P.
Araus, Jose Luis
Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation
title Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation
title_full Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation
title_fullStr Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation
title_full_unstemmed Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation
title_short Photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation
title_sort photosynthetic contribution of the ear to grain filling in wheat: a comparison of different methodologies for evaluation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861024/
https://www.ncbi.nlm.nih.gov/pubmed/27012283
http://dx.doi.org/10.1093/jxb/erw116
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