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Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay

Currently available behavioral assays to quantify normal cold sensitivity, cold hypersensitivity and cold hyperalgesia in mice have betimes created conflicting results in the literature. Some only capture a limited spectrum of thermal experiences, others are prone to experimenter bias or are not sen...

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Autores principales: Touska, Filip, Winter, Zoltán, Mueller, Alexander, Vlachova, Viktorie, Larsen, Jonas, Zimmermann, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861200/
https://www.ncbi.nlm.nih.gov/pubmed/27227099
http://dx.doi.org/10.1080/23328940.2015.1135689
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author Touska, Filip
Winter, Zoltán
Mueller, Alexander
Vlachova, Viktorie
Larsen, Jonas
Zimmermann, Katharina
author_facet Touska, Filip
Winter, Zoltán
Mueller, Alexander
Vlachova, Viktorie
Larsen, Jonas
Zimmermann, Katharina
author_sort Touska, Filip
collection PubMed
description Currently available behavioral assays to quantify normal cold sensitivity, cold hypersensitivity and cold hyperalgesia in mice have betimes created conflicting results in the literature. Some only capture a limited spectrum of thermal experiences, others are prone to experimenter bias or are not sensitive enough to detect the contribution of ion channels to cold sensing because in mice smaller alterations in cold nociception do not manifest as frank behavioral changes. To overcome current limitations we have designed a novel device that is automated, provides a high degree of freedom, i.e. thermal choice, and eliminates experimenter bias. The device represents a thermal gradient assay designed as a circular running track. It allows discerning exploratory behavior from thermal selection behavior and provides increased accuracy by providing measured values in duplicate and by removing edge artifacts. Our custom-designed automated offline analysis by a blob detection algorithm is devoid of movement artifacts, removes light reflection artifacts and provides an internal quality control parameter which we validated. The assay delivers discrete information on a large range of parameters extracted from the occupancy of thermally defined zones such as preference temperature and skew of the distribution. We demonstrate that the assay allows increasingly accurate phenotyping of thermal sensitivity in transgenic mice by disclosing yet unrecognized details on the phenotypes of TRPM8-, TRPA1- and TRPM8/A1-deficient mice.
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spelling pubmed-48612002016-05-25 Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay Touska, Filip Winter, Zoltán Mueller, Alexander Vlachova, Viktorie Larsen, Jonas Zimmermann, Katharina Temperature (Austin) Method Articles Currently available behavioral assays to quantify normal cold sensitivity, cold hypersensitivity and cold hyperalgesia in mice have betimes created conflicting results in the literature. Some only capture a limited spectrum of thermal experiences, others are prone to experimenter bias or are not sensitive enough to detect the contribution of ion channels to cold sensing because in mice smaller alterations in cold nociception do not manifest as frank behavioral changes. To overcome current limitations we have designed a novel device that is automated, provides a high degree of freedom, i.e. thermal choice, and eliminates experimenter bias. The device represents a thermal gradient assay designed as a circular running track. It allows discerning exploratory behavior from thermal selection behavior and provides increased accuracy by providing measured values in duplicate and by removing edge artifacts. Our custom-designed automated offline analysis by a blob detection algorithm is devoid of movement artifacts, removes light reflection artifacts and provides an internal quality control parameter which we validated. The assay delivers discrete information on a large range of parameters extracted from the occupancy of thermally defined zones such as preference temperature and skew of the distribution. We demonstrate that the assay allows increasingly accurate phenotyping of thermal sensitivity in transgenic mice by disclosing yet unrecognized details on the phenotypes of TRPM8-, TRPA1- and TRPM8/A1-deficient mice. Taylor & Francis 2016-03-02 /pmc/articles/PMC4861200/ /pubmed/27227099 http://dx.doi.org/10.1080/23328940.2015.1135689 Text en © 2016 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Method Articles
Touska, Filip
Winter, Zoltán
Mueller, Alexander
Vlachova, Viktorie
Larsen, Jonas
Zimmermann, Katharina
Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay
title Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay
title_full Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay
title_fullStr Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay
title_full_unstemmed Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay
title_short Comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay
title_sort comprehensive thermal preference phenotyping in mice using a novel automated circular gradient assay
topic Method Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861200/
https://www.ncbi.nlm.nih.gov/pubmed/27227099
http://dx.doi.org/10.1080/23328940.2015.1135689
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