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Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection
Next-generation sequencing has identified novel astroviruses for which a pathogenic role is not clearly defined. We identified astrovirus MLB2 infection in an immunocompetent case-patient and an immunocompromised patient who experienced diverse clinical manifestations, notably, meningitis and dissem...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861523/ https://www.ncbi.nlm.nih.gov/pubmed/27088842 http://dx.doi.org/10.3201/eid2205.151807 |
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author | Cordey, Samuel Vu, Diem-Lan Schibler, Manuel L’Huillier, Arnaud G. Brito, Francisco Docquier, Mylène Posfay-Barbe, Klara M. Petty, Thomas J. Turin, Lara Zdobnov, Evgeny M. Kaiser, Laurent |
author_facet | Cordey, Samuel Vu, Diem-Lan Schibler, Manuel L’Huillier, Arnaud G. Brito, Francisco Docquier, Mylène Posfay-Barbe, Klara M. Petty, Thomas J. Turin, Lara Zdobnov, Evgeny M. Kaiser, Laurent |
author_sort | Cordey, Samuel |
collection | PubMed |
description | Next-generation sequencing has identified novel astroviruses for which a pathogenic role is not clearly defined. We identified astrovirus MLB2 infection in an immunocompetent case-patient and an immunocompromised patient who experienced diverse clinical manifestations, notably, meningitis and disseminated infection. The initial case-patient was identified by next-generation sequencing, which revealed astrovirus MLB2 RNA in cerebrospinal fluid, plasma, urine, and anal swab specimens. We then used specific real-time reverse transcription PCR to screen 943 fecal and 424 cerebrospinal fluid samples from hospitalized patients and identified a second case of meningitis, with positive results for the agent in the patient’s feces and plasma. This screening revealed 5 additional positive fecal samples: 1 from an infant with acute diarrhea and 4 from children who had received transplants. Our findings demonstrate that astrovirus MLB2, which is highly prevalent in feces, can disseminate outside the digestive tract and is an unrecognized cause of central nervous system infection. |
format | Online Article Text |
id | pubmed-4861523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-48615232016-05-10 Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection Cordey, Samuel Vu, Diem-Lan Schibler, Manuel L’Huillier, Arnaud G. Brito, Francisco Docquier, Mylène Posfay-Barbe, Klara M. Petty, Thomas J. Turin, Lara Zdobnov, Evgeny M. Kaiser, Laurent Emerg Infect Dis Research Next-generation sequencing has identified novel astroviruses for which a pathogenic role is not clearly defined. We identified astrovirus MLB2 infection in an immunocompetent case-patient and an immunocompromised patient who experienced diverse clinical manifestations, notably, meningitis and disseminated infection. The initial case-patient was identified by next-generation sequencing, which revealed astrovirus MLB2 RNA in cerebrospinal fluid, plasma, urine, and anal swab specimens. We then used specific real-time reverse transcription PCR to screen 943 fecal and 424 cerebrospinal fluid samples from hospitalized patients and identified a second case of meningitis, with positive results for the agent in the patient’s feces and plasma. This screening revealed 5 additional positive fecal samples: 1 from an infant with acute diarrhea and 4 from children who had received transplants. Our findings demonstrate that astrovirus MLB2, which is highly prevalent in feces, can disseminate outside the digestive tract and is an unrecognized cause of central nervous system infection. Centers for Disease Control and Prevention 2016-05 /pmc/articles/PMC4861523/ /pubmed/27088842 http://dx.doi.org/10.3201/eid2205.151807 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Cordey, Samuel Vu, Diem-Lan Schibler, Manuel L’Huillier, Arnaud G. Brito, Francisco Docquier, Mylène Posfay-Barbe, Klara M. Petty, Thomas J. Turin, Lara Zdobnov, Evgeny M. Kaiser, Laurent Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection |
title | Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection |
title_full | Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection |
title_fullStr | Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection |
title_full_unstemmed | Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection |
title_short | Astrovirus MLB2, a New Gastroenteric Virus Associated with Meningitis and Disseminated Infection |
title_sort | astrovirus mlb2, a new gastroenteric virus associated with meningitis and disseminated infection |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861523/ https://www.ncbi.nlm.nih.gov/pubmed/27088842 http://dx.doi.org/10.3201/eid2205.151807 |
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