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Adult axolotls can regenerate original neuronal diversity in response to brain injury
The axolotl can regenerate multiple organs, including the brain. It remains, however, unclear whether neuronal diversity, intricate tissue architecture, and axonal connectivity can be regenerated; yet, this is critical for recovery of function and a central aim of cell replacement strategies in the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861602/ https://www.ncbi.nlm.nih.gov/pubmed/27156560 http://dx.doi.org/10.7554/eLife.13998 |
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author | Amamoto, Ryoji Huerta, Violeta Gisselle Lopez Takahashi, Emi Dai, Guangping Grant, Aaron K Fu, Zhanyan Arlotta, Paola |
author_facet | Amamoto, Ryoji Huerta, Violeta Gisselle Lopez Takahashi, Emi Dai, Guangping Grant, Aaron K Fu, Zhanyan Arlotta, Paola |
author_sort | Amamoto, Ryoji |
collection | PubMed |
description | The axolotl can regenerate multiple organs, including the brain. It remains, however, unclear whether neuronal diversity, intricate tissue architecture, and axonal connectivity can be regenerated; yet, this is critical for recovery of function and a central aim of cell replacement strategies in the mammalian central nervous system. Here, we demonstrate that, upon mechanical injury to the adult pallium, axolotls can regenerate several of the populations of neurons present before injury. Notably, regenerated neurons acquire functional electrophysiological traits and respond appropriately to afferent inputs. Despite the ability to regenerate specific, molecularly-defined neuronal subtypes, we also uncovered previously unappreciated limitations by showing that newborn neurons organize within altered tissue architecture and fail to re-establish the long-distance axonal tracts and circuit physiology present before injury. The data provide a direct demonstration that diverse, electrophysiologically functional neurons can be regenerated in axolotls, but challenge prior assumptions of functional brain repair in regenerative species. DOI: http://dx.doi.org/10.7554/eLife.13998.001 |
format | Online Article Text |
id | pubmed-4861602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-48616022016-05-11 Adult axolotls can regenerate original neuronal diversity in response to brain injury Amamoto, Ryoji Huerta, Violeta Gisselle Lopez Takahashi, Emi Dai, Guangping Grant, Aaron K Fu, Zhanyan Arlotta, Paola eLife Developmental Biology and Stem Cells The axolotl can regenerate multiple organs, including the brain. It remains, however, unclear whether neuronal diversity, intricate tissue architecture, and axonal connectivity can be regenerated; yet, this is critical for recovery of function and a central aim of cell replacement strategies in the mammalian central nervous system. Here, we demonstrate that, upon mechanical injury to the adult pallium, axolotls can regenerate several of the populations of neurons present before injury. Notably, regenerated neurons acquire functional electrophysiological traits and respond appropriately to afferent inputs. Despite the ability to regenerate specific, molecularly-defined neuronal subtypes, we also uncovered previously unappreciated limitations by showing that newborn neurons organize within altered tissue architecture and fail to re-establish the long-distance axonal tracts and circuit physiology present before injury. The data provide a direct demonstration that diverse, electrophysiologically functional neurons can be regenerated in axolotls, but challenge prior assumptions of functional brain repair in regenerative species. DOI: http://dx.doi.org/10.7554/eLife.13998.001 eLife Sciences Publications, Ltd 2016-05-09 /pmc/articles/PMC4861602/ /pubmed/27156560 http://dx.doi.org/10.7554/eLife.13998 Text en © 2016, Amamoto et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Amamoto, Ryoji Huerta, Violeta Gisselle Lopez Takahashi, Emi Dai, Guangping Grant, Aaron K Fu, Zhanyan Arlotta, Paola Adult axolotls can regenerate original neuronal diversity in response to brain injury |
title | Adult axolotls can regenerate original neuronal diversity in response to brain injury |
title_full | Adult axolotls can regenerate original neuronal diversity in response to brain injury |
title_fullStr | Adult axolotls can regenerate original neuronal diversity in response to brain injury |
title_full_unstemmed | Adult axolotls can regenerate original neuronal diversity in response to brain injury |
title_short | Adult axolotls can regenerate original neuronal diversity in response to brain injury |
title_sort | adult axolotls can regenerate original neuronal diversity in response to brain injury |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861602/ https://www.ncbi.nlm.nih.gov/pubmed/27156560 http://dx.doi.org/10.7554/eLife.13998 |
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