Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural...

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Autores principales: Fukusumi, Hayato, Shofuda, Tomoko, Bamba, Yohei, Yamamoto, Atsuyo, Kanematsu, Daisuke, Handa, Yukako, Okita, Keisuke, Nakamura, Masaya, Yamanaka, Shinya, Okano, Hideyuki, Kanemura, Yonehiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861799/
https://www.ncbi.nlm.nih.gov/pubmed/27212953
http://dx.doi.org/10.1155/2016/7235757
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author Fukusumi, Hayato
Shofuda, Tomoko
Bamba, Yohei
Yamamoto, Atsuyo
Kanematsu, Daisuke
Handa, Yukako
Okita, Keisuke
Nakamura, Masaya
Yamanaka, Shinya
Okano, Hideyuki
Kanemura, Yonehiro
author_facet Fukusumi, Hayato
Shofuda, Tomoko
Bamba, Yohei
Yamamoto, Atsuyo
Kanematsu, Daisuke
Handa, Yukako
Okita, Keisuke
Nakamura, Masaya
Yamanaka, Shinya
Okano, Hideyuki
Kanemura, Yonehiro
author_sort Fukusumi, Hayato
collection PubMed
description Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.
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spelling pubmed-48617992016-05-22 Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins Fukusumi, Hayato Shofuda, Tomoko Bamba, Yohei Yamamoto, Atsuyo Kanematsu, Daisuke Handa, Yukako Okita, Keisuke Nakamura, Masaya Yamanaka, Shinya Okano, Hideyuki Kanemura, Yonehiro Stem Cells Int Research Article Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes. Hindawi Publishing Corporation 2016 2016-04-26 /pmc/articles/PMC4861799/ /pubmed/27212953 http://dx.doi.org/10.1155/2016/7235757 Text en Copyright © 2016 Hayato Fukusumi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fukusumi, Hayato
Shofuda, Tomoko
Bamba, Yohei
Yamamoto, Atsuyo
Kanematsu, Daisuke
Handa, Yukako
Okita, Keisuke
Nakamura, Masaya
Yamanaka, Shinya
Okano, Hideyuki
Kanemura, Yonehiro
Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
title Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
title_full Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
title_fullStr Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
title_full_unstemmed Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
title_short Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins
title_sort establishment of human neural progenitor cells from human induced pluripotent stem cells with diverse tissue origins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861799/
https://www.ncbi.nlm.nih.gov/pubmed/27212953
http://dx.doi.org/10.1155/2016/7235757
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