Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity

Increasing evidence indicates that lncRNAs play critical roles in various biological processes, but many have not been functionally characterized. Here, we report a novel radiation-inducible lncRNA, namely lnc-RI which is essential for cell survival and appropriate mitotic progression. Our data indi...

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Autores principales: Wang, Zhi-Dong, Shen, Li-Ping, Chang, Cheng, Zhang, Xue-Qing, Chen, Zhong-Min, Li, Lin, Chen, Hong, Zhou, Ping-Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861912/
https://www.ncbi.nlm.nih.gov/pubmed/27160062
http://dx.doi.org/10.1038/srep25385
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author Wang, Zhi-Dong
Shen, Li-Ping
Chang, Cheng
Zhang, Xue-Qing
Chen, Zhong-Min
Li, Lin
Chen, Hong
Zhou, Ping-Kun
author_facet Wang, Zhi-Dong
Shen, Li-Ping
Chang, Cheng
Zhang, Xue-Qing
Chen, Zhong-Min
Li, Lin
Chen, Hong
Zhou, Ping-Kun
author_sort Wang, Zhi-Dong
collection PubMed
description Increasing evidence indicates that lncRNAs play critical roles in various biological processes, but many have not been functionally characterized. Here, we report a novel radiation-inducible lncRNA, namely lnc-RI which is essential for cell survival and appropriate mitotic progression. Our data indicated that knockdown of lnc-RI resulted in spindle abnormalities and mitotic arrest simultaneously with sharply decreased mRNA and protein expression of PLK1, a key regulator of mitosis. Our data demonstrated that PLK1 is a key downstream mediator of lnc-RI in regulating mitosis, whereby lnc-RI competitively bound to the negative PLK1 regulating miRNA, miRNA-210-p3. Taken together, we have identified lnc-RI as a new regulator of mitosis which acts by releasing PLK1 mRNA activity via competition for binding to miRNA-210-3p.
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spelling pubmed-48619122016-05-20 Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity Wang, Zhi-Dong Shen, Li-Ping Chang, Cheng Zhang, Xue-Qing Chen, Zhong-Min Li, Lin Chen, Hong Zhou, Ping-Kun Sci Rep Article Increasing evidence indicates that lncRNAs play critical roles in various biological processes, but many have not been functionally characterized. Here, we report a novel radiation-inducible lncRNA, namely lnc-RI which is essential for cell survival and appropriate mitotic progression. Our data indicated that knockdown of lnc-RI resulted in spindle abnormalities and mitotic arrest simultaneously with sharply decreased mRNA and protein expression of PLK1, a key regulator of mitosis. Our data demonstrated that PLK1 is a key downstream mediator of lnc-RI in regulating mitosis, whereby lnc-RI competitively bound to the negative PLK1 regulating miRNA, miRNA-210-p3. Taken together, we have identified lnc-RI as a new regulator of mitosis which acts by releasing PLK1 mRNA activity via competition for binding to miRNA-210-3p. Nature Publishing Group 2016-05-10 /pmc/articles/PMC4861912/ /pubmed/27160062 http://dx.doi.org/10.1038/srep25385 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Zhi-Dong
Shen, Li-Ping
Chang, Cheng
Zhang, Xue-Qing
Chen, Zhong-Min
Li, Lin
Chen, Hong
Zhou, Ping-Kun
Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity
title Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity
title_full Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity
title_fullStr Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity
title_full_unstemmed Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity
title_short Long noncoding RNA lnc-RI is a new regulator of mitosis via targeting miRNA-210-3p to release PLK1 mRNA activity
title_sort long noncoding rna lnc-ri is a new regulator of mitosis via targeting mirna-210-3p to release plk1 mrna activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4861912/
https://www.ncbi.nlm.nih.gov/pubmed/27160062
http://dx.doi.org/10.1038/srep25385
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