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Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes

The purpose of this study was to assess the effect of three different inoculation routes into mycoplasmal pneumonia (MP) in pigs challenged with Mycoplasma hyopneumoniae (M. hyopneumoniae). Thirty six-week-old M. hyopneumoniae seronegative piglets were randomly assigned to four groups: three challen...

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Autores principales: Garcia-Morante, Beatriz, Segalés, Joaquim, López-Soria, Sergio, de Rozas, Ana Pérez, Maiti, Henrike, Coll, Teresa, Sibila, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4862235/
https://www.ncbi.nlm.nih.gov/pubmed/27160189
http://dx.doi.org/10.1186/s13567-016-0340-2
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author Garcia-Morante, Beatriz
Segalés, Joaquim
López-Soria, Sergio
de Rozas, Ana Pérez
Maiti, Henrike
Coll, Teresa
Sibila, Marina
author_facet Garcia-Morante, Beatriz
Segalés, Joaquim
López-Soria, Sergio
de Rozas, Ana Pérez
Maiti, Henrike
Coll, Teresa
Sibila, Marina
author_sort Garcia-Morante, Beatriz
collection PubMed
description The purpose of this study was to assess the effect of three different inoculation routes into mycoplasmal pneumonia (MP) in pigs challenged with Mycoplasma hyopneumoniae (M. hyopneumoniae). Thirty six-week-old M. hyopneumoniae seronegative piglets were randomly assigned to four groups: three challenged groups with experimentally inoculated pigs by either the endotracheal (ET; n = 8), intranasal (IN; n = 8) or aerosol (AE; n = 8) routes and one uninfected group (Control; n = 6). Blood samples were collected 1 day before challenge and at necropsy, 28 days post-inoculation (dpi), to assess seroconversion. Laryngeal swabs were collected at −1, 7, 14, 21 and 28 dpi in order to evaluate colonization. At necropsy, lung lesions were scored and lung tissue was collected for histopathological studies and M. hyopneumoniae DNA detection. Broncho-alveolar lavage fluid (BALF) was also obtained to detect M. hyopneumoniae DNA, specific IgA antibodies and cytokines. MP was observed in all inoculated groups, but the ET group displayed a significantly higher number of animals affected by MP as well as a higher mean lung lesion score. These results were paralleled with an earlier seroconversion and upper respiratory tract colonization of M. hyopneumoniae. Additionally, in the ET group, higher levels of pro-inflammatory cytokines and specific IgA antibodies in BALF were found. Under the conditions of the present study, MP was reproduced by the three evaluated inoculation routes. Obtained results suggest that the ET route is the most effective in order to induce MP in pigs experimentally challenged with M. hyopneumoniae.
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spelling pubmed-48622352016-05-11 Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes Garcia-Morante, Beatriz Segalés, Joaquim López-Soria, Sergio de Rozas, Ana Pérez Maiti, Henrike Coll, Teresa Sibila, Marina Vet Res Research Article The purpose of this study was to assess the effect of three different inoculation routes into mycoplasmal pneumonia (MP) in pigs challenged with Mycoplasma hyopneumoniae (M. hyopneumoniae). Thirty six-week-old M. hyopneumoniae seronegative piglets were randomly assigned to four groups: three challenged groups with experimentally inoculated pigs by either the endotracheal (ET; n = 8), intranasal (IN; n = 8) or aerosol (AE; n = 8) routes and one uninfected group (Control; n = 6). Blood samples were collected 1 day before challenge and at necropsy, 28 days post-inoculation (dpi), to assess seroconversion. Laryngeal swabs were collected at −1, 7, 14, 21 and 28 dpi in order to evaluate colonization. At necropsy, lung lesions were scored and lung tissue was collected for histopathological studies and M. hyopneumoniae DNA detection. Broncho-alveolar lavage fluid (BALF) was also obtained to detect M. hyopneumoniae DNA, specific IgA antibodies and cytokines. MP was observed in all inoculated groups, but the ET group displayed a significantly higher number of animals affected by MP as well as a higher mean lung lesion score. These results were paralleled with an earlier seroconversion and upper respiratory tract colonization of M. hyopneumoniae. Additionally, in the ET group, higher levels of pro-inflammatory cytokines and specific IgA antibodies in BALF were found. Under the conditions of the present study, MP was reproduced by the three evaluated inoculation routes. Obtained results suggest that the ET route is the most effective in order to induce MP in pigs experimentally challenged with M. hyopneumoniae. BioMed Central 2016-05-09 2016 /pmc/articles/PMC4862235/ /pubmed/27160189 http://dx.doi.org/10.1186/s13567-016-0340-2 Text en © Garcia-Morante et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Garcia-Morante, Beatriz
Segalés, Joaquim
López-Soria, Sergio
de Rozas, Ana Pérez
Maiti, Henrike
Coll, Teresa
Sibila, Marina
Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes
title Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes
title_full Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes
title_fullStr Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes
title_full_unstemmed Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes
title_short Induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes
title_sort induction of mycoplasmal pneumonia in experimentally infected pigs by means of different inoculation routes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4862235/
https://www.ncbi.nlm.nih.gov/pubmed/27160189
http://dx.doi.org/10.1186/s13567-016-0340-2
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