Cargando…
Seroepidemiology of Plasmodium species infections in Zimbabwean population
BACKGROUND: Individuals living in malaria-endemic regions may be exposed to more than one Plasmodium species; there is paucity of data on the distribution of the different species of Plasmodium in affected populations, in part due to the diagnostic method of microscopy, which cannot easily different...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863323/ https://www.ncbi.nlm.nih.gov/pubmed/27165412 http://dx.doi.org/10.1186/s12936-016-1325-3 |
_version_ | 1782431462685933568 |
---|---|
author | Amanfo, Seth A. Mduluza, Takafira Midzi, Nicholas Cavanagh, David R. Mutapi, Francisca |
author_facet | Amanfo, Seth A. Mduluza, Takafira Midzi, Nicholas Cavanagh, David R. Mutapi, Francisca |
author_sort | Amanfo, Seth A. |
collection | PubMed |
description | BACKGROUND: Individuals living in malaria-endemic regions may be exposed to more than one Plasmodium species; there is paucity of data on the distribution of the different species of Plasmodium in affected populations, in part due to the diagnostic method of microscopy, which cannot easily differentiate between the species. Sero-epidemiological data can overcome some of the shortcomings of microscopy. METHODS: The specificity of IgG antibodies to recombinant merozoite surface protein 1 (MSP-1(19)) derived from four human Plasmodium species (Plasmodiumfalciparum, Plasmodiumvivax, Plasmodiummalariae, Plasmodiumovale) was investigated using competition enzyme-linked immunosorbent assay. Subsequently, these antigens were used to determine the exposure prevalence to the different Plasmodium species in serum samples of participants. One-hundred individuals, aged five-18 years, from each of the three Plasmodium meso-endemic Zimbabwean villages (Burma Valley, Mutoko, Chiredzi) were recruited in the study. RESULTS: The study demonstrated that the host serum reactivity to MSP-1(19) antigens was species-specific and that no cross-reactivity occurred. The overall prevalence of antibody response to MSP-1(19) antigens was 61 % in Burma Valley, 31 % in Mutoko and 32 % in Chiredzi. Single species IgG responses to MSP-1(19) were most frequent against P. falciparum, followed by P. malariae and P. ovale, with responses to P. vivax being the least prevalent. Interestingly, 78–87 and 50 % of sera with IgG responses to P. malariae and P. ovale MSP-1(19), respectively, also had IgG specific response for P. falciparum MSP-1(19) antigens, indicating that exposure to these species is a common occurrence in these populations. Single species IgG responses to the non-falciparum species were at a very low frequency, ranging between 0 and 13 % for P. malariae. CONCLUSIONS: There is evidence of a higher exposure to the non-falciparum parasite species than previously reported in Zimbabwe. The recombinant MSP-1(19) antigens could be used as additional diagnostic tools in antibody assays for the detection of exposure to the different Plasmodium species. The results also introduce an interesting concept of the co-infection of non-falciparum Plasmodium almost always with P. falciparum, which requires further validation and mechanistic studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12936-016-1325-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4863323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48633232016-05-12 Seroepidemiology of Plasmodium species infections in Zimbabwean population Amanfo, Seth A. Mduluza, Takafira Midzi, Nicholas Cavanagh, David R. Mutapi, Francisca Malar J Research BACKGROUND: Individuals living in malaria-endemic regions may be exposed to more than one Plasmodium species; there is paucity of data on the distribution of the different species of Plasmodium in affected populations, in part due to the diagnostic method of microscopy, which cannot easily differentiate between the species. Sero-epidemiological data can overcome some of the shortcomings of microscopy. METHODS: The specificity of IgG antibodies to recombinant merozoite surface protein 1 (MSP-1(19)) derived from four human Plasmodium species (Plasmodiumfalciparum, Plasmodiumvivax, Plasmodiummalariae, Plasmodiumovale) was investigated using competition enzyme-linked immunosorbent assay. Subsequently, these antigens were used to determine the exposure prevalence to the different Plasmodium species in serum samples of participants. One-hundred individuals, aged five-18 years, from each of the three Plasmodium meso-endemic Zimbabwean villages (Burma Valley, Mutoko, Chiredzi) were recruited in the study. RESULTS: The study demonstrated that the host serum reactivity to MSP-1(19) antigens was species-specific and that no cross-reactivity occurred. The overall prevalence of antibody response to MSP-1(19) antigens was 61 % in Burma Valley, 31 % in Mutoko and 32 % in Chiredzi. Single species IgG responses to MSP-1(19) were most frequent against P. falciparum, followed by P. malariae and P. ovale, with responses to P. vivax being the least prevalent. Interestingly, 78–87 and 50 % of sera with IgG responses to P. malariae and P. ovale MSP-1(19), respectively, also had IgG specific response for P. falciparum MSP-1(19) antigens, indicating that exposure to these species is a common occurrence in these populations. Single species IgG responses to the non-falciparum species were at a very low frequency, ranging between 0 and 13 % for P. malariae. CONCLUSIONS: There is evidence of a higher exposure to the non-falciparum parasite species than previously reported in Zimbabwe. The recombinant MSP-1(19) antigens could be used as additional diagnostic tools in antibody assays for the detection of exposure to the different Plasmodium species. The results also introduce an interesting concept of the co-infection of non-falciparum Plasmodium almost always with P. falciparum, which requires further validation and mechanistic studies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12936-016-1325-3) contains supplementary material, which is available to authorized users. BioMed Central 2016-05-10 /pmc/articles/PMC4863323/ /pubmed/27165412 http://dx.doi.org/10.1186/s12936-016-1325-3 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Amanfo, Seth A. Mduluza, Takafira Midzi, Nicholas Cavanagh, David R. Mutapi, Francisca Seroepidemiology of Plasmodium species infections in Zimbabwean population |
title | Seroepidemiology of Plasmodium species infections in Zimbabwean population |
title_full | Seroepidemiology of Plasmodium species infections in Zimbabwean population |
title_fullStr | Seroepidemiology of Plasmodium species infections in Zimbabwean population |
title_full_unstemmed | Seroepidemiology of Plasmodium species infections in Zimbabwean population |
title_short | Seroepidemiology of Plasmodium species infections in Zimbabwean population |
title_sort | seroepidemiology of plasmodium species infections in zimbabwean population |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863323/ https://www.ncbi.nlm.nih.gov/pubmed/27165412 http://dx.doi.org/10.1186/s12936-016-1325-3 |
work_keys_str_mv | AT amanfosetha seroepidemiologyofplasmodiumspeciesinfectionsinzimbabweanpopulation AT mduluzatakafira seroepidemiologyofplasmodiumspeciesinfectionsinzimbabweanpopulation AT midzinicholas seroepidemiologyofplasmodiumspeciesinfectionsinzimbabweanpopulation AT cavanaghdavidr seroepidemiologyofplasmodiumspeciesinfectionsinzimbabweanpopulation AT mutapifrancisca seroepidemiologyofplasmodiumspeciesinfectionsinzimbabweanpopulation |