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High-level Expression and Purification of Active Human FGF-2 in Escherichia coli by Codon and Culture Condition Optimization

BACKGROUND: Basic fibroblast growth factor (bFGF) is a member of a highly conserved superfamily of proteins that are involved in cell proliferation, differentiation, and migration. OBJECTIVES: The objective of this study was to overexpress and purify the high-level active human bFGF in Escherichia c...

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Detalles Bibliográficos
Autores principales:	Soleyman, Mohammad Reza, Khalili, Mostafa, Khansarinejad, Behzad, Baazm, Maryam
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Kowsar 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863364/ https://www.ncbi.nlm.nih.gov/pubmed/27175305 http://dx.doi.org/10.5812/ircmj.21615

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