Cargando…

Structural investigation into physiological DNA phosphorothioate modification

DNA phosphorothioate (PT) modification, with sulfur replacing a nonbridging phosphate oxygen in a sequence and stereo specific manner, is a novel physiological variation in bacteria. But what effects on DNA properties PT modification has is still unclear. To address this, we prepared three double-st...

Descripción completa

Detalles Bibliográficos
Autores principales: Lan, Wenxian, Hu, Zhongpei, Shen, Jie, Wang, Chunxi, Jiang, Feng, Liu, Huili, Long, Dewu, Liu, Maili, Cao, Chunyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864419/
https://www.ncbi.nlm.nih.gov/pubmed/27169778
http://dx.doi.org/10.1038/srep25737
_version_ 1782431625338945536
author Lan, Wenxian
Hu, Zhongpei
Shen, Jie
Wang, Chunxi
Jiang, Feng
Liu, Huili
Long, Dewu
Liu, Maili
Cao, Chunyang
author_facet Lan, Wenxian
Hu, Zhongpei
Shen, Jie
Wang, Chunxi
Jiang, Feng
Liu, Huili
Long, Dewu
Liu, Maili
Cao, Chunyang
author_sort Lan, Wenxian
collection PubMed
description DNA phosphorothioate (PT) modification, with sulfur replacing a nonbridging phosphate oxygen in a sequence and stereo specific manner, is a novel physiological variation in bacteria. But what effects on DNA properties PT modification has is still unclear. To address this, we prepared three double-stranded (ds) DNA decamers, d(CG(PX)GCCGCCGA) with its complementary strand d(TCGGCG(PX)GCCG) (where X = O or S, i.e., PT-free dsDNA, [S(p), S(p)]-PT dsDNA or [R(p), R(p)]-PT dsDNA) located in gene of Streptomyces lividans. Their melting temperature (T(m)) measurement indicates that [R(p), R(p)]-PT dsDNA is most unstable. Their electron transfer potential detection presents an order of anti-oxidation properties: S(p)-PT DNA > R(p)-PT DNA > PT-free DNA. Their NMR structures demonstrate that PT modification doesn’t change their B-form conformation. The sulfur in [R(p), R(p)]-PT dsDNA locates in the major groove, with steric effects on protons in the sugar close to modification sites, resulting in its unstability, and facilitating its selectively interactions with ScoMcrA. We thought that PT modification was dialectical to the bacteria. It protects the hosting bacteria by working as antioxidant against H(2)O(2), and acts as a marker, directing restriction enzyme observed in other hosts, like ScoMcrA, to correctly cleave the PT modified DNA, so that bacteria cannot spread and survive.
format Online
Article
Text
id pubmed-4864419
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-48644192016-05-23 Structural investigation into physiological DNA phosphorothioate modification Lan, Wenxian Hu, Zhongpei Shen, Jie Wang, Chunxi Jiang, Feng Liu, Huili Long, Dewu Liu, Maili Cao, Chunyang Sci Rep Article DNA phosphorothioate (PT) modification, with sulfur replacing a nonbridging phosphate oxygen in a sequence and stereo specific manner, is a novel physiological variation in bacteria. But what effects on DNA properties PT modification has is still unclear. To address this, we prepared three double-stranded (ds) DNA decamers, d(CG(PX)GCCGCCGA) with its complementary strand d(TCGGCG(PX)GCCG) (where X = O or S, i.e., PT-free dsDNA, [S(p), S(p)]-PT dsDNA or [R(p), R(p)]-PT dsDNA) located in gene of Streptomyces lividans. Their melting temperature (T(m)) measurement indicates that [R(p), R(p)]-PT dsDNA is most unstable. Their electron transfer potential detection presents an order of anti-oxidation properties: S(p)-PT DNA > R(p)-PT DNA > PT-free DNA. Their NMR structures demonstrate that PT modification doesn’t change their B-form conformation. The sulfur in [R(p), R(p)]-PT dsDNA locates in the major groove, with steric effects on protons in the sugar close to modification sites, resulting in its unstability, and facilitating its selectively interactions with ScoMcrA. We thought that PT modification was dialectical to the bacteria. It protects the hosting bacteria by working as antioxidant against H(2)O(2), and acts as a marker, directing restriction enzyme observed in other hosts, like ScoMcrA, to correctly cleave the PT modified DNA, so that bacteria cannot spread and survive. Nature Publishing Group 2016-05-12 /pmc/articles/PMC4864419/ /pubmed/27169778 http://dx.doi.org/10.1038/srep25737 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lan, Wenxian
Hu, Zhongpei
Shen, Jie
Wang, Chunxi
Jiang, Feng
Liu, Huili
Long, Dewu
Liu, Maili
Cao, Chunyang
Structural investigation into physiological DNA phosphorothioate modification
title Structural investigation into physiological DNA phosphorothioate modification
title_full Structural investigation into physiological DNA phosphorothioate modification
title_fullStr Structural investigation into physiological DNA phosphorothioate modification
title_full_unstemmed Structural investigation into physiological DNA phosphorothioate modification
title_short Structural investigation into physiological DNA phosphorothioate modification
title_sort structural investigation into physiological dna phosphorothioate modification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864419/
https://www.ncbi.nlm.nih.gov/pubmed/27169778
http://dx.doi.org/10.1038/srep25737
work_keys_str_mv AT lanwenxian structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT huzhongpei structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT shenjie structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT wangchunxi structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT jiangfeng structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT liuhuili structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT longdewu structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT liumaili structuralinvestigationintophysiologicaldnaphosphorothioatemodification
AT caochunyang structuralinvestigationintophysiologicaldnaphosphorothioatemodification