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Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids

Dietary polyunsaturated fatty acids (PUFA) are suggested to modulate immune function, but the effects of dietary fatty acids composition on gene expression patterns in immune organs have not been fully characterized. In the current study we investigated how dietary fatty acids composition affects th...

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Autores principales: Svahn, Sara L., Väremo, Leif, Gabrielsson, Britt G., Peris, Eduard, Nookaew, Intawat, Grahnemo, Louise, Sandberg, Ann-Sofie, Wernstedt Asterholm, Ingrid, Jansson, John-Olov, Nielsen, Jens, Johansson, Maria E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864434/
https://www.ncbi.nlm.nih.gov/pubmed/27166587
http://dx.doi.org/10.1371/journal.pone.0155099
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author Svahn, Sara L.
Väremo, Leif
Gabrielsson, Britt G.
Peris, Eduard
Nookaew, Intawat
Grahnemo, Louise
Sandberg, Ann-Sofie
Wernstedt Asterholm, Ingrid
Jansson, John-Olov
Nielsen, Jens
Johansson, Maria E.
author_facet Svahn, Sara L.
Väremo, Leif
Gabrielsson, Britt G.
Peris, Eduard
Nookaew, Intawat
Grahnemo, Louise
Sandberg, Ann-Sofie
Wernstedt Asterholm, Ingrid
Jansson, John-Olov
Nielsen, Jens
Johansson, Maria E.
author_sort Svahn, Sara L.
collection PubMed
description Dietary polyunsaturated fatty acids (PUFA) are suggested to modulate immune function, but the effects of dietary fatty acids composition on gene expression patterns in immune organs have not been fully characterized. In the current study we investigated how dietary fatty acids composition affects the total transcriptome profile, and especially, immune related genes in two immune organs, spleen (SPL) and bone marrow cells (BMC). Four tissues with metabolic function, skeletal muscle (SKM), white adipose tissue (WAT), brown adipose tissue (BAT), and liver (LIV), were investigated as a comparison. Following 8 weeks on low fat diet (LFD), high fat diet (HFD) rich in saturated fatty acids (HFD-S), or HFD rich in PUFA (HFD-P), tissue transcriptomics were analyzed by microarray and metabolic health assessed by fasting blood glucose level, HOMA-IR index, oral glucose tolerance test as well as quantification of crown-like structures in WAT. HFD-P corrected the metabolic phenotype induced by HFD-S. Interestingly, SKM and BMC were relatively inert to the diets, whereas the two adipose tissues (WAT and BAT) were mainly affected by HFD per se (both HFD-S and HFD-P). In particular, WAT gene expression was driven closer to that of the immune organs SPL and BMC by HFDs. The LIV exhibited different responses to both of the HFDs. Surprisingly, the spleen showed a major response to HFD-P (82 genes differed from LFD, mostly immune genes), while it was not affected at all by HFD-S (0 genes differed from LFD). In conclusion, the quantity and composition of dietary fatty acids affected the transcriptome in distinct manners in different organs. Remarkably, dietary PUFA, but not saturated fat, prompted a specific regulation of immune related genes in the spleen, opening the possibility that PUFA can regulate immune function by influencing gene expression in this organ.
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spelling pubmed-48644342016-05-18 Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids Svahn, Sara L. Väremo, Leif Gabrielsson, Britt G. Peris, Eduard Nookaew, Intawat Grahnemo, Louise Sandberg, Ann-Sofie Wernstedt Asterholm, Ingrid Jansson, John-Olov Nielsen, Jens Johansson, Maria E. PLoS One Research Article Dietary polyunsaturated fatty acids (PUFA) are suggested to modulate immune function, but the effects of dietary fatty acids composition on gene expression patterns in immune organs have not been fully characterized. In the current study we investigated how dietary fatty acids composition affects the total transcriptome profile, and especially, immune related genes in two immune organs, spleen (SPL) and bone marrow cells (BMC). Four tissues with metabolic function, skeletal muscle (SKM), white adipose tissue (WAT), brown adipose tissue (BAT), and liver (LIV), were investigated as a comparison. Following 8 weeks on low fat diet (LFD), high fat diet (HFD) rich in saturated fatty acids (HFD-S), or HFD rich in PUFA (HFD-P), tissue transcriptomics were analyzed by microarray and metabolic health assessed by fasting blood glucose level, HOMA-IR index, oral glucose tolerance test as well as quantification of crown-like structures in WAT. HFD-P corrected the metabolic phenotype induced by HFD-S. Interestingly, SKM and BMC were relatively inert to the diets, whereas the two adipose tissues (WAT and BAT) were mainly affected by HFD per se (both HFD-S and HFD-P). In particular, WAT gene expression was driven closer to that of the immune organs SPL and BMC by HFDs. The LIV exhibited different responses to both of the HFDs. Surprisingly, the spleen showed a major response to HFD-P (82 genes differed from LFD, mostly immune genes), while it was not affected at all by HFD-S (0 genes differed from LFD). In conclusion, the quantity and composition of dietary fatty acids affected the transcriptome in distinct manners in different organs. Remarkably, dietary PUFA, but not saturated fat, prompted a specific regulation of immune related genes in the spleen, opening the possibility that PUFA can regulate immune function by influencing gene expression in this organ. Public Library of Science 2016-05-11 /pmc/articles/PMC4864434/ /pubmed/27166587 http://dx.doi.org/10.1371/journal.pone.0155099 Text en © 2016 Svahn et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Svahn, Sara L.
Väremo, Leif
Gabrielsson, Britt G.
Peris, Eduard
Nookaew, Intawat
Grahnemo, Louise
Sandberg, Ann-Sofie
Wernstedt Asterholm, Ingrid
Jansson, John-Olov
Nielsen, Jens
Johansson, Maria E.
Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids
title Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids
title_full Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids
title_fullStr Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids
title_full_unstemmed Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids
title_short Six Tissue Transcriptomics Reveals Specific Immune Suppression in Spleen by Dietary Polyunsaturated Fatty Acids
title_sort six tissue transcriptomics reveals specific immune suppression in spleen by dietary polyunsaturated fatty acids
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864434/
https://www.ncbi.nlm.nih.gov/pubmed/27166587
http://dx.doi.org/10.1371/journal.pone.0155099
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