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C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes
C4.4A is a modular glycolipid-anchored Ly6/uPAR/α-neurotoxin multidomain protein that exhibits a prominent membrane-associated expression in stratified squamous epithelia. C4.4A is also expressed in various solid cancer lesions, where high expression levels often are correlated to poor prognosis. Ci...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864438/ https://www.ncbi.nlm.nih.gov/pubmed/27169360 http://dx.doi.org/10.1038/srep25833 |
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author | Kriegbaum, Mette Camilla Jacobsen, Benedikte Füchtbauer, Annette Hansen, Gert Helge Christensen, Ib Jarle Rundsten, Carsten Friis Persson, Morten Engelholm, Lars Henning Madsen, Andreas Nygaard Di Meo, Ivano Lund, Ida Katrine Holst, Birgitte Kjaer, Andreas Lærum, Ole Didrik Füchtbauer, Ernst-Martin Ploug, Michael |
author_facet | Kriegbaum, Mette Camilla Jacobsen, Benedikte Füchtbauer, Annette Hansen, Gert Helge Christensen, Ib Jarle Rundsten, Carsten Friis Persson, Morten Engelholm, Lars Henning Madsen, Andreas Nygaard Di Meo, Ivano Lund, Ida Katrine Holst, Birgitte Kjaer, Andreas Lærum, Ole Didrik Füchtbauer, Ernst-Martin Ploug, Michael |
author_sort | Kriegbaum, Mette Camilla |
collection | PubMed |
description | C4.4A is a modular glycolipid-anchored Ly6/uPAR/α-neurotoxin multidomain protein that exhibits a prominent membrane-associated expression in stratified squamous epithelia. C4.4A is also expressed in various solid cancer lesions, where high expression levels often are correlated to poor prognosis. Circumstantial evidence suggests a role for C4.4A in cell adhesion, migration, and invasion, but a well-defined biological function is currently unknown. In the present study, we have generated and characterized the first C4.4A-deficient mouse line to gain insight into the functional significance of C4.4A in normal physiology and cancer progression. The unchallenged C4.4A-deficient mice were viable, fertile, born in a normal Mendelian distribution and, surprisingly, displayed normal development of squamous epithelia. The C4.4A-deficient mice were, nonetheless, significantly lighter than littermate controls predominantly due to differences in fat mass. Congenital C4.4A deficiency delayed migration of keratinocytes enclosing incisional skin wounds in male mice. In chemically induced bladder carcinomas, C4.4A deficiency attenuated the incidence of invasive lesions despite having no effect on total tumour burden. This new C4.4A-deficient mouse line provides a useful platform for future studies on functional aspects of C4.4A in tumour cell invasion in vivo. |
format | Online Article Text |
id | pubmed-4864438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48644382016-05-23 C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes Kriegbaum, Mette Camilla Jacobsen, Benedikte Füchtbauer, Annette Hansen, Gert Helge Christensen, Ib Jarle Rundsten, Carsten Friis Persson, Morten Engelholm, Lars Henning Madsen, Andreas Nygaard Di Meo, Ivano Lund, Ida Katrine Holst, Birgitte Kjaer, Andreas Lærum, Ole Didrik Füchtbauer, Ernst-Martin Ploug, Michael Sci Rep Article C4.4A is a modular glycolipid-anchored Ly6/uPAR/α-neurotoxin multidomain protein that exhibits a prominent membrane-associated expression in stratified squamous epithelia. C4.4A is also expressed in various solid cancer lesions, where high expression levels often are correlated to poor prognosis. Circumstantial evidence suggests a role for C4.4A in cell adhesion, migration, and invasion, but a well-defined biological function is currently unknown. In the present study, we have generated and characterized the first C4.4A-deficient mouse line to gain insight into the functional significance of C4.4A in normal physiology and cancer progression. The unchallenged C4.4A-deficient mice were viable, fertile, born in a normal Mendelian distribution and, surprisingly, displayed normal development of squamous epithelia. The C4.4A-deficient mice were, nonetheless, significantly lighter than littermate controls predominantly due to differences in fat mass. Congenital C4.4A deficiency delayed migration of keratinocytes enclosing incisional skin wounds in male mice. In chemically induced bladder carcinomas, C4.4A deficiency attenuated the incidence of invasive lesions despite having no effect on total tumour burden. This new C4.4A-deficient mouse line provides a useful platform for future studies on functional aspects of C4.4A in tumour cell invasion in vivo. Nature Publishing Group 2016-05-12 /pmc/articles/PMC4864438/ /pubmed/27169360 http://dx.doi.org/10.1038/srep25833 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kriegbaum, Mette Camilla Jacobsen, Benedikte Füchtbauer, Annette Hansen, Gert Helge Christensen, Ib Jarle Rundsten, Carsten Friis Persson, Morten Engelholm, Lars Henning Madsen, Andreas Nygaard Di Meo, Ivano Lund, Ida Katrine Holst, Birgitte Kjaer, Andreas Lærum, Ole Didrik Füchtbauer, Ernst-Martin Ploug, Michael C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes |
title | C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes |
title_full | C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes |
title_fullStr | C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes |
title_full_unstemmed | C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes |
title_short | C4.4A gene ablation is compatible with normal epidermal development and causes modest overt phenotypes |
title_sort | c4.4a gene ablation is compatible with normal epidermal development and causes modest overt phenotypes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864438/ https://www.ncbi.nlm.nih.gov/pubmed/27169360 http://dx.doi.org/10.1038/srep25833 |
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