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Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis
AIM: The virulence-associated protein-A of Staphylococcus aureus, encoded by spa gene shows a variation in length in different strains. In this study, the spa gene variation in S. aureus strains was studied which were isolated from subclinical cases of bovine mastitis. MATERIALS AND METHODS: About 3...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864486/ https://www.ncbi.nlm.nih.gov/pubmed/27182140 http://dx.doi.org/10.14202/vetworld.2016.421-424 |
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author | Bhati, Taruna Nathawat, Prerna Sharma, Sandeep Kumar Yadav, Rahul Bishnoi, Jyoti Kataria, Anil Kumar |
author_facet | Bhati, Taruna Nathawat, Prerna Sharma, Sandeep Kumar Yadav, Rahul Bishnoi, Jyoti Kataria, Anil Kumar |
author_sort | Bhati, Taruna |
collection | PubMed |
description | AIM: The virulence-associated protein-A of Staphylococcus aureus, encoded by spa gene shows a variation in length in different strains. In this study, the spa gene variation in S. aureus strains was studied which were isolated from subclinical cases of bovine mastitis. MATERIALS AND METHODS: About 38 isolatesof S. aureus were recovered from Holstein–Friesian (HF) crossbred (n=16) and Rathi cattle (n=22) with subclinical mastitis as per standard procedures, and these isolates were subjected to amplification of spa gene (X-region) by polymerase chain reaction and calculation of number of tandem repeats were done. RESULTS: Of the 16 isolates from H-F crossbred cattle, all with the exception of one isolate produced spa amplicon. Seven isolates produced amplicons of 200 bp, one produced 160 bp, and other seven produced spa amplicon of 150 bp with calculated number of 6, 5, and 4 repeats, respectively, whereas nine different types of amplicons were produced by 22 S. aureus isolates from Rathi cattle, viz., 280, 250, 240, 200, 190, 180, 170, 150, and 140 bp with 10, 8, 8, 6, 6, 6, 5, 4, and 4 repeats, respectively. One of the isolates from Rathi cattle produced two spa amplicons (150 and 190 bp). CONCLUSION: A greater polymorphism was observed in the S. aureus isolates from Rathi cattle than from H-F crossbreds with subclinical mastitis. |
format | Online Article Text |
id | pubmed-4864486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-48644862016-05-13 Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis Bhati, Taruna Nathawat, Prerna Sharma, Sandeep Kumar Yadav, Rahul Bishnoi, Jyoti Kataria, Anil Kumar Vet World Research Article AIM: The virulence-associated protein-A of Staphylococcus aureus, encoded by spa gene shows a variation in length in different strains. In this study, the spa gene variation in S. aureus strains was studied which were isolated from subclinical cases of bovine mastitis. MATERIALS AND METHODS: About 38 isolatesof S. aureus were recovered from Holstein–Friesian (HF) crossbred (n=16) and Rathi cattle (n=22) with subclinical mastitis as per standard procedures, and these isolates were subjected to amplification of spa gene (X-region) by polymerase chain reaction and calculation of number of tandem repeats were done. RESULTS: Of the 16 isolates from H-F crossbred cattle, all with the exception of one isolate produced spa amplicon. Seven isolates produced amplicons of 200 bp, one produced 160 bp, and other seven produced spa amplicon of 150 bp with calculated number of 6, 5, and 4 repeats, respectively, whereas nine different types of amplicons were produced by 22 S. aureus isolates from Rathi cattle, viz., 280, 250, 240, 200, 190, 180, 170, 150, and 140 bp with 10, 8, 8, 6, 6, 6, 5, 4, and 4 repeats, respectively. One of the isolates from Rathi cattle produced two spa amplicons (150 and 190 bp). CONCLUSION: A greater polymorphism was observed in the S. aureus isolates from Rathi cattle than from H-F crossbreds with subclinical mastitis. Veterinary World 2016-04 2016-04-28 /pmc/articles/PMC4864486/ /pubmed/27182140 http://dx.doi.org/10.14202/vetworld.2016.421-424 Text en Copyright: © Bhati, et al. http://creativecommons.org/licenses/by/4.0 Copyright: Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Bhati, Taruna Nathawat, Prerna Sharma, Sandeep Kumar Yadav, Rahul Bishnoi, Jyoti Kataria, Anil Kumar Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis |
title | Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis |
title_full | Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis |
title_fullStr | Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis |
title_full_unstemmed | Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis |
title_short | Polymorphism in spa gene of Staphylococcus aureus from bovine subclinical mastitis |
title_sort | polymorphism in spa gene of staphylococcus aureus from bovine subclinical mastitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864486/ https://www.ncbi.nlm.nih.gov/pubmed/27182140 http://dx.doi.org/10.14202/vetworld.2016.421-424 |
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