Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential

Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequenci...

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Autores principales: Akita, Hironaga, Kimura, Zen-ichiro, Mohd Yusoff, Mohd Zulkhairi, Nakashima, Nobutaka, Hoshino, Tamotsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864794/
https://www.ncbi.nlm.nih.gov/pubmed/27247892
http://dx.doi.org/10.1186/s40064-016-2237-y
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author Akita, Hironaga
Kimura, Zen-ichiro
Mohd Yusoff, Mohd Zulkhairi
Nakashima, Nobutaka
Hoshino, Tamotsu
author_facet Akita, Hironaga
Kimura, Zen-ichiro
Mohd Yusoff, Mohd Zulkhairi
Nakashima, Nobutaka
Hoshino, Tamotsu
author_sort Akita, Hironaga
collection PubMed
description Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation.
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spelling pubmed-48647942016-05-31 Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential Akita, Hironaga Kimura, Zen-ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu Springerplus Research Microbial degradation of lignin releases fermentable sugars, effective utilization of which could support biofuel production from lignocellulosic biomass. In the present study, a lignin-degrading bacterium was isolated from leaf soil and identified as Burkholderia sp. based on 16S rRNA gene sequencing. This strain was named CCA53, and its lignin-degrading capability was assessed by observing its growth on medium containing alkali lignin or lignin-associated aromatic monomers as the sole carbon source. Alkali lignin and at least eight lignin-associated aromatic monomers supported growth of this strain, and the most effective utilization was observed for p-hydroxybenzene monomers. These findings indicate that Burkholderia sp. strain CCA53 has fragmentary activity for lignin degradation. Springer International Publishing 2016-05-11 /pmc/articles/PMC4864794/ /pubmed/27247892 http://dx.doi.org/10.1186/s40064-016-2237-y Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Akita, Hironaga
Kimura, Zen-ichiro
Mohd Yusoff, Mohd Zulkhairi
Nakashima, Nobutaka
Hoshino, Tamotsu
Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential
title Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential
title_full Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential
title_fullStr Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential
title_full_unstemmed Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential
title_short Isolation and characterization of Burkholderia sp. strain CCA53 exhibiting ligninolytic potential
title_sort isolation and characterization of burkholderia sp. strain cca53 exhibiting ligninolytic potential
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864794/
https://www.ncbi.nlm.nih.gov/pubmed/27247892
http://dx.doi.org/10.1186/s40064-016-2237-y
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