Cargando…

Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and thus influence many cellular and physiological processes. miRNAs are also present in cell-free body fluids such as plasma or serum, and these circulating miRNAs are very stable, sensitive, and specific biomarkers of patho...

Descripción completa

Detalles Bibliográficos
Autores principales: Salamin, Olivier, Jaggi, Laetitia, Baume, Norbert, Robinson, Neil, Saugy, Martial, Leuenberger, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865044/
https://www.ncbi.nlm.nih.gov/pubmed/27171140
http://dx.doi.org/10.1371/journal.pone.0155248
_version_ 1782431720770895872
author Salamin, Olivier
Jaggi, Laetitia
Baume, Norbert
Robinson, Neil
Saugy, Martial
Leuenberger, Nicolas
author_facet Salamin, Olivier
Jaggi, Laetitia
Baume, Norbert
Robinson, Neil
Saugy, Martial
Leuenberger, Nicolas
author_sort Salamin, Olivier
collection PubMed
description MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and thus influence many cellular and physiological processes. miRNAs are also present in cell-free body fluids such as plasma or serum, and these circulating miRNAs are very stable, sensitive, and specific biomarkers of pathophysiological states. In this study, we investigated whether circulating miRNAs could serve as biomarkers of exogenous testosterone administration. Misuse of testosterone as a performance-enhancing drug is thought to be widespread in sports. Detection of testosterone through the urinary steroid profile of the Athlete Biological Passport faces several obstacles, indicating that new biomarkers are required. To this end, we analyzed plasma miRNA levels by high-throughput quantitative real-time PCR. Plasma samples were obtained before and at several time points after transdermal and oral testosterone administration. Screening identified three potential candidate miRNAs that were altered by both routes of testosterone administration. Longitudinal monitoring of these candidates revealed that variation in two of them (miR-150 and miR-342), relative to the corresponding levels in control samples, was testosterone-independent. However, levels of the liver-specific miR-122 increased 3.5-fold 1 day after drug intake. Given that testosterone is metabolized by the liver, this observation suggests that miR-122 in cell-free fluids may be used as a sensitive biomarker of testosterone misuse via multiple dosing routes and could therefore be integrated into a blood-based multiparametric follow-up.
format Online
Article
Text
id pubmed-4865044
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-48650442016-05-26 Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse Salamin, Olivier Jaggi, Laetitia Baume, Norbert Robinson, Neil Saugy, Martial Leuenberger, Nicolas PLoS One Research Article MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and thus influence many cellular and physiological processes. miRNAs are also present in cell-free body fluids such as plasma or serum, and these circulating miRNAs are very stable, sensitive, and specific biomarkers of pathophysiological states. In this study, we investigated whether circulating miRNAs could serve as biomarkers of exogenous testosterone administration. Misuse of testosterone as a performance-enhancing drug is thought to be widespread in sports. Detection of testosterone through the urinary steroid profile of the Athlete Biological Passport faces several obstacles, indicating that new biomarkers are required. To this end, we analyzed plasma miRNA levels by high-throughput quantitative real-time PCR. Plasma samples were obtained before and at several time points after transdermal and oral testosterone administration. Screening identified three potential candidate miRNAs that were altered by both routes of testosterone administration. Longitudinal monitoring of these candidates revealed that variation in two of them (miR-150 and miR-342), relative to the corresponding levels in control samples, was testosterone-independent. However, levels of the liver-specific miR-122 increased 3.5-fold 1 day after drug intake. Given that testosterone is metabolized by the liver, this observation suggests that miR-122 in cell-free fluids may be used as a sensitive biomarker of testosterone misuse via multiple dosing routes and could therefore be integrated into a blood-based multiparametric follow-up. Public Library of Science 2016-05-12 /pmc/articles/PMC4865044/ /pubmed/27171140 http://dx.doi.org/10.1371/journal.pone.0155248 Text en © 2016 Salamin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Salamin, Olivier
Jaggi, Laetitia
Baume, Norbert
Robinson, Neil
Saugy, Martial
Leuenberger, Nicolas
Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse
title Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse
title_full Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse
title_fullStr Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse
title_full_unstemmed Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse
title_short Circulating microRNA-122 as Potential Biomarker for Detection of Testosterone Abuse
title_sort circulating microrna-122 as potential biomarker for detection of testosterone abuse
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865044/
https://www.ncbi.nlm.nih.gov/pubmed/27171140
http://dx.doi.org/10.1371/journal.pone.0155248
work_keys_str_mv AT salaminolivier circulatingmicrorna122aspotentialbiomarkerfordetectionoftestosteroneabuse
AT jaggilaetitia circulatingmicrorna122aspotentialbiomarkerfordetectionoftestosteroneabuse
AT baumenorbert circulatingmicrorna122aspotentialbiomarkerfordetectionoftestosteroneabuse
AT robinsonneil circulatingmicrorna122aspotentialbiomarkerfordetectionoftestosteroneabuse
AT saugymartial circulatingmicrorna122aspotentialbiomarkerfordetectionoftestosteroneabuse
AT leuenbergernicolas circulatingmicrorna122aspotentialbiomarkerfordetectionoftestosteroneabuse