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Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils

It is important to have tools to measure the bioavailability to assess the risks of pollutants because the bioavailability is defined as the portions of pollutants showing the biological effects on living organisms. This study described the construction of tunable Escherichia coli whole-cell biorepo...

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Autores principales: Yoon, Youngdae, Kim, Sunghoon, Chae, Yooeun, Kang, Yerin, Lee, Youngshim, Jeong, Seung-Woo, An, Youn-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865175/
https://www.ncbi.nlm.nih.gov/pubmed/27171374
http://dx.doi.org/10.1371/journal.pone.0154506
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author Yoon, Youngdae
Kim, Sunghoon
Chae, Yooeun
Kang, Yerin
Lee, Youngshim
Jeong, Seung-Woo
An, Youn-Joo
author_facet Yoon, Youngdae
Kim, Sunghoon
Chae, Yooeun
Kang, Yerin
Lee, Youngshim
Jeong, Seung-Woo
An, Youn-Joo
author_sort Yoon, Youngdae
collection PubMed
description It is important to have tools to measure the bioavailability to assess the risks of pollutants because the bioavailability is defined as the portions of pollutants showing the biological effects on living organisms. This study described the construction of tunable Escherichia coli whole-cell bioreporter (WCB) using the promoter region of zinc-inducible operon and its application on contaminated soils. It was verified that this WCB system showed specific and sensitive responses to cadmium rather than zinc in the experimental conditions. It was inferred that Cd(II) associates stronger with ZntR, a regulatory protein of zinc-inducible operon, than other metal ions. Moreover, the expression of reporter genes, egfp and mcherry, were proportional to the concentration of cadmium, thereby being a quantitative sensor to monitor bioavailable cadmium. The capability to determine bioavailable cadmium was verified with Cd(II) amended LUFA soils, and then the applicability on environmental systems was investigated with field soils collected from smelter area in Korea before and after soil-washing. The total amount of cadmium was decreased after soil washing, while the bioavailability was increased. Consequently, it would be valuable to have tools to assess bioavailability and the effectiveness of soil remediation should be evaluated in the aspect of bioavailability as well as removal efficiency.
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spelling pubmed-48651752016-05-26 Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils Yoon, Youngdae Kim, Sunghoon Chae, Yooeun Kang, Yerin Lee, Youngshim Jeong, Seung-Woo An, Youn-Joo PLoS One Research Article It is important to have tools to measure the bioavailability to assess the risks of pollutants because the bioavailability is defined as the portions of pollutants showing the biological effects on living organisms. This study described the construction of tunable Escherichia coli whole-cell bioreporter (WCB) using the promoter region of zinc-inducible operon and its application on contaminated soils. It was verified that this WCB system showed specific and sensitive responses to cadmium rather than zinc in the experimental conditions. It was inferred that Cd(II) associates stronger with ZntR, a regulatory protein of zinc-inducible operon, than other metal ions. Moreover, the expression of reporter genes, egfp and mcherry, were proportional to the concentration of cadmium, thereby being a quantitative sensor to monitor bioavailable cadmium. The capability to determine bioavailable cadmium was verified with Cd(II) amended LUFA soils, and then the applicability on environmental systems was investigated with field soils collected from smelter area in Korea before and after soil-washing. The total amount of cadmium was decreased after soil washing, while the bioavailability was increased. Consequently, it would be valuable to have tools to assess bioavailability and the effectiveness of soil remediation should be evaluated in the aspect of bioavailability as well as removal efficiency. Public Library of Science 2016-05-12 /pmc/articles/PMC4865175/ /pubmed/27171374 http://dx.doi.org/10.1371/journal.pone.0154506 Text en © 2016 Yoon et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yoon, Youngdae
Kim, Sunghoon
Chae, Yooeun
Kang, Yerin
Lee, Youngshim
Jeong, Seung-Woo
An, Youn-Joo
Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils
title Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils
title_full Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils
title_fullStr Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils
title_full_unstemmed Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils
title_short Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils
title_sort use of tunable whole-cell bioreporters to assess bioavailable cadmium and remediation performance in soils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865175/
https://www.ncbi.nlm.nih.gov/pubmed/27171374
http://dx.doi.org/10.1371/journal.pone.0154506
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