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Differences in environmental stress response among yeasts is consistent with species-specific lifestyles

Defining how organisms respond to environmental change has always been an important step toward understanding their adaptive capacity and physiology. Variation in transcription during stress has been widely described in model species, especially in the yeast Saccharomyces cerevisiae, which helped to...

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Autores principales: Brion, Christian, Pflieger, David, Souali-Crespo, Sirine, Friedrich, Anne, Schacherer, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865325/
https://www.ncbi.nlm.nih.gov/pubmed/27009200
http://dx.doi.org/10.1091/mbc.E15-12-0816
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author Brion, Christian
Pflieger, David
Souali-Crespo, Sirine
Friedrich, Anne
Schacherer, Joseph
author_facet Brion, Christian
Pflieger, David
Souali-Crespo, Sirine
Friedrich, Anne
Schacherer, Joseph
author_sort Brion, Christian
collection PubMed
description Defining how organisms respond to environmental change has always been an important step toward understanding their adaptive capacity and physiology. Variation in transcription during stress has been widely described in model species, especially in the yeast Saccharomyces cerevisiae, which helped to shape general rules regarding how cells cope with environmental constraints, as well as to decipher the functions of many genes. Comparison of the environmental stress response (ESR) across species is essential to obtaining better insight into the common and species-specific features of stress defense. In this context, we explored the transcriptional landscape of the yeast Lachancea kluyveri (formerly Saccharomyces kluyveri) in response to diverse stresses, using RNA sequencing. We investigated variation in gene expression and observed a link between genetic plasticity and environmental sensitivity. We identified the ESR genes in this species and compared them to those already found in S. cerevisiae. We observed common features between the two species, as well as divergence in the regulatory networks involved. Of interest, some changes were related to differences in species lifestyle. Thus we were able to decipher how adaptation to stress has evolved among different yeast species. Finally, by analyzing patterns of coexpression, we were able to propose potential biological functions for 42% of genes and also annotate 301 genes for which no function could be assigned by homology. This large data set allowed for the characterization of the evolution of gene regulation and provides an efficient tool for assessing gene function.
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spelling pubmed-48653252016-07-30 Differences in environmental stress response among yeasts is consistent with species-specific lifestyles Brion, Christian Pflieger, David Souali-Crespo, Sirine Friedrich, Anne Schacherer, Joseph Mol Biol Cell Articles Defining how organisms respond to environmental change has always been an important step toward understanding their adaptive capacity and physiology. Variation in transcription during stress has been widely described in model species, especially in the yeast Saccharomyces cerevisiae, which helped to shape general rules regarding how cells cope with environmental constraints, as well as to decipher the functions of many genes. Comparison of the environmental stress response (ESR) across species is essential to obtaining better insight into the common and species-specific features of stress defense. In this context, we explored the transcriptional landscape of the yeast Lachancea kluyveri (formerly Saccharomyces kluyveri) in response to diverse stresses, using RNA sequencing. We investigated variation in gene expression and observed a link between genetic plasticity and environmental sensitivity. We identified the ESR genes in this species and compared them to those already found in S. cerevisiae. We observed common features between the two species, as well as divergence in the regulatory networks involved. Of interest, some changes were related to differences in species lifestyle. Thus we were able to decipher how adaptation to stress has evolved among different yeast species. Finally, by analyzing patterns of coexpression, we were able to propose potential biological functions for 42% of genes and also annotate 301 genes for which no function could be assigned by homology. This large data set allowed for the characterization of the evolution of gene regulation and provides an efficient tool for assessing gene function. The American Society for Cell Biology 2016-05-15 /pmc/articles/PMC4865325/ /pubmed/27009200 http://dx.doi.org/10.1091/mbc.E15-12-0816 Text en © 2016 Brion et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Brion, Christian
Pflieger, David
Souali-Crespo, Sirine
Friedrich, Anne
Schacherer, Joseph
Differences in environmental stress response among yeasts is consistent with species-specific lifestyles
title Differences in environmental stress response among yeasts is consistent with species-specific lifestyles
title_full Differences in environmental stress response among yeasts is consistent with species-specific lifestyles
title_fullStr Differences in environmental stress response among yeasts is consistent with species-specific lifestyles
title_full_unstemmed Differences in environmental stress response among yeasts is consistent with species-specific lifestyles
title_short Differences in environmental stress response among yeasts is consistent with species-specific lifestyles
title_sort differences in environmental stress response among yeasts is consistent with species-specific lifestyles
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865325/
https://www.ncbi.nlm.nih.gov/pubmed/27009200
http://dx.doi.org/10.1091/mbc.E15-12-0816
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