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The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development
Radial intercalation is a fundamental process responsible for the thinning of multilayered tissues during large-scale morphogenesis; however, its molecular mechanism has remained elusive. Using amphibian epiboly, the thinning and spreading of the animal hemisphere during gastrulation, here we provid...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865533/ https://www.ncbi.nlm.nih.gov/pubmed/27165554 http://dx.doi.org/10.1016/j.devcel.2016.04.008 |
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author | Szabó, András Cobo, Isidoro Omara, Sharif McLachlan, Sophie Keller, Ray Mayor, Roberto |
author_facet | Szabó, András Cobo, Isidoro Omara, Sharif McLachlan, Sophie Keller, Ray Mayor, Roberto |
author_sort | Szabó, András |
collection | PubMed |
description | Radial intercalation is a fundamental process responsible for the thinning of multilayered tissues during large-scale morphogenesis; however, its molecular mechanism has remained elusive. Using amphibian epiboly, the thinning and spreading of the animal hemisphere during gastrulation, here we provide evidence that radial intercalation is driven by chemotaxis of cells toward the external layer of the tissue. This role of chemotaxis in tissue spreading and thinning is unlike its typical role associated with large-distance directional movement of cells. We identify the chemoattractant as the complement component C3a, a factor normally linked with the immune system. The mechanism is explored by computational modeling and tested in vivo, ex vivo, and in vitro. This mechanism is robust against fluctuations of chemoattractant levels and expression patterns and explains expansion during epiboly. This study provides insight into the fundamental process of radial intercalation and could be applied to a wide range of morphogenetic events. |
format | Online Article Text |
id | pubmed-4865533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48655332016-05-23 The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development Szabó, András Cobo, Isidoro Omara, Sharif McLachlan, Sophie Keller, Ray Mayor, Roberto Dev Cell Article Radial intercalation is a fundamental process responsible for the thinning of multilayered tissues during large-scale morphogenesis; however, its molecular mechanism has remained elusive. Using amphibian epiboly, the thinning and spreading of the animal hemisphere during gastrulation, here we provide evidence that radial intercalation is driven by chemotaxis of cells toward the external layer of the tissue. This role of chemotaxis in tissue spreading and thinning is unlike its typical role associated with large-distance directional movement of cells. We identify the chemoattractant as the complement component C3a, a factor normally linked with the immune system. The mechanism is explored by computational modeling and tested in vivo, ex vivo, and in vitro. This mechanism is robust against fluctuations of chemoattractant levels and expression patterns and explains expansion during epiboly. This study provides insight into the fundamental process of radial intercalation and could be applied to a wide range of morphogenetic events. Cell Press 2016-05-09 /pmc/articles/PMC4865533/ /pubmed/27165554 http://dx.doi.org/10.1016/j.devcel.2016.04.008 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Szabó, András Cobo, Isidoro Omara, Sharif McLachlan, Sophie Keller, Ray Mayor, Roberto The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development |
title | The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development |
title_full | The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development |
title_fullStr | The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development |
title_full_unstemmed | The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development |
title_short | The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development |
title_sort | molecular basis of radial intercalation during tissue spreading in early development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865533/ https://www.ncbi.nlm.nih.gov/pubmed/27165554 http://dx.doi.org/10.1016/j.devcel.2016.04.008 |
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