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Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorptio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865669/ https://www.ncbi.nlm.nih.gov/pubmed/27222848 http://dx.doi.org/10.1016/j.dib.2016.04.042 |
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author | Kang, Ji-Yeon Kwon, Ohsuk Gil, Jin Young Oh, Doo-Byoung |
author_facet | Kang, Ji-Yeon Kwon, Ohsuk Gil, Jin Young Oh, Doo-Byoung |
author_sort | Kang, Ji-Yeon |
collection | PubMed |
description | Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The identities of M-6-P glycan peaks in HPLC profile were confirmed by measuring the masses of the collected peak eluates. The performances of three fluorescent tags (2-aminobenzoic acid [2-AA], 2-aminobenzamide [2-AB], and 3-(acetyl-amino)-6-aminoacridine [AA-Ac]) were compared focusing on the analysis of bi-phosphorylated glycan (containing two M-6-Ps). The bi-phosphorylated glycan analysis is highly affected by the attached fluorescent tag and the hydrophilicity of elution solvent used in HPLC. The data in this article is associated with the research article published in “Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans” (Kang et al., 2016 [1]). |
format | Online Article Text |
id | pubmed-4865669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48656692016-05-24 Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags Kang, Ji-Yeon Kwon, Ohsuk Gil, Jin Young Oh, Doo-Byoung Data Brief Data Article Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The identities of M-6-P glycan peaks in HPLC profile were confirmed by measuring the masses of the collected peak eluates. The performances of three fluorescent tags (2-aminobenzoic acid [2-AA], 2-aminobenzamide [2-AB], and 3-(acetyl-amino)-6-aminoacridine [AA-Ac]) were compared focusing on the analysis of bi-phosphorylated glycan (containing two M-6-Ps). The bi-phosphorylated glycan analysis is highly affected by the attached fluorescent tag and the hydrophilicity of elution solvent used in HPLC. The data in this article is associated with the research article published in “Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans” (Kang et al., 2016 [1]). Elsevier 2016-04-23 /pmc/articles/PMC4865669/ /pubmed/27222848 http://dx.doi.org/10.1016/j.dib.2016.04.042 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Kang, Ji-Yeon Kwon, Ohsuk Gil, Jin Young Oh, Doo-Byoung Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags |
title | Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags |
title_full | Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags |
title_fullStr | Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags |
title_full_unstemmed | Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags |
title_short | Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags |
title_sort | data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865669/ https://www.ncbi.nlm.nih.gov/pubmed/27222848 http://dx.doi.org/10.1016/j.dib.2016.04.042 |
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