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Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags

Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorptio...

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Autores principales: Kang, Ji-Yeon, Kwon, Ohsuk, Gil, Jin Young, Oh, Doo-Byoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865669/
https://www.ncbi.nlm.nih.gov/pubmed/27222848
http://dx.doi.org/10.1016/j.dib.2016.04.042
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author Kang, Ji-Yeon
Kwon, Ohsuk
Gil, Jin Young
Oh, Doo-Byoung
author_facet Kang, Ji-Yeon
Kwon, Ohsuk
Gil, Jin Young
Oh, Doo-Byoung
author_sort Kang, Ji-Yeon
collection PubMed
description Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The identities of M-6-P glycan peaks in HPLC profile were confirmed by measuring the masses of the collected peak eluates. The performances of three fluorescent tags (2-aminobenzoic acid [2-AA], 2-aminobenzamide [2-AB], and 3-(acetyl-amino)-6-aminoacridine [AA-Ac]) were compared focusing on the analysis of bi-phosphorylated glycan (containing two M-6-Ps). The bi-phosphorylated glycan analysis is highly affected by the attached fluorescent tag and the hydrophilicity of elution solvent used in HPLC. The data in this article is associated with the research article published in “Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans” (Kang et al., 2016 [1]).
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spelling pubmed-48656692016-05-24 Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags Kang, Ji-Yeon Kwon, Ohsuk Gil, Jin Young Oh, Doo-Byoung Data Brief Data Article Mannose-6-phosphate (M-6-P) glycan plays an important role in lysosomal targeting of most therapeutic enzymes for treatment of lysosomal storage diseases. This article provides data for the analysis of M-6-P glycans by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The identities of M-6-P glycan peaks in HPLC profile were confirmed by measuring the masses of the collected peak eluates. The performances of three fluorescent tags (2-aminobenzoic acid [2-AA], 2-aminobenzamide [2-AB], and 3-(acetyl-amino)-6-aminoacridine [AA-Ac]) were compared focusing on the analysis of bi-phosphorylated glycan (containing two M-6-Ps). The bi-phosphorylated glycan analysis is highly affected by the attached fluorescent tag and the hydrophilicity of elution solvent used in HPLC. The data in this article is associated with the research article published in “Comparison of fluorescent tags for analysis of mannose-6-phosphate glycans” (Kang et al., 2016 [1]). Elsevier 2016-04-23 /pmc/articles/PMC4865669/ /pubmed/27222848 http://dx.doi.org/10.1016/j.dib.2016.04.042 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Kang, Ji-Yeon
Kwon, Ohsuk
Gil, Jin Young
Oh, Doo-Byoung
Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
title Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
title_full Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
title_fullStr Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
title_full_unstemmed Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
title_short Data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
title_sort data for analysis of mannose-6-phosphate glycans labeled with fluorescent tags
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865669/
https://www.ncbi.nlm.nih.gov/pubmed/27222848
http://dx.doi.org/10.1016/j.dib.2016.04.042
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