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Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution
BACKGROUND: Obesity and low high-density lipoprotein-cholesterol (HDL-C) levels are associated with cardiovascular risk. Surprisingly, despite a greater prevalence of obesity and lower HDL concentrations than white women, black South African women are relatively protected against ischaemic heart dis...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866302/ https://www.ncbi.nlm.nih.gov/pubmed/27169717 http://dx.doi.org/10.1186/s12944-016-0257-9 |
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author | Woudberg, Nicholas J. Goedecke, Julia H. Blackhurst, Dee Frias, Miguel James, Richard Opie, Lionel H. Lecour, Sandrine |
author_facet | Woudberg, Nicholas J. Goedecke, Julia H. Blackhurst, Dee Frias, Miguel James, Richard Opie, Lionel H. Lecour, Sandrine |
author_sort | Woudberg, Nicholas J. |
collection | PubMed |
description | BACKGROUND: Obesity and low high-density lipoprotein-cholesterol (HDL-C) levels are associated with cardiovascular risk. Surprisingly, despite a greater prevalence of obesity and lower HDL concentrations than white women, black South African women are relatively protected against ischaemic heart disease. METHODS: We investigated whether this apparent discrepancy may be related to different HDL function and subclass distribution in black and white, normal-weight and obese South African women (n = 40). HDL functionality was assessed by measuring paraoxonase (PON) activity, platelet activating factor acetylhydrolase (PAF-AH) activity, Oxygen Radical Absorbance Capacity (ORAC) and quantification of the expression of vascular cell adhesion molecule in endothelial cells. PON-1 and PAF-AH expression was determined in isolated HDL and serum using Western blotting. Levels of large, intermediate and small HDL subclasses were measured using the Lipoprint® system. RESULTS: PON activity was lower in white compared to black women (0.49 ± 0.09 U/L vs 0.78 ± 0.10 U/L, p < 0.05), regardless of PON-1 protein levels. Obese black women had lower PAF-AH activity (9.34 ± 1.15 U/L vs 13.89 ± 1.21 U/L, p <0.05) and HDL-associated PAF-AH expression compared to obese white women. Compared to normal-weight women, obese women had lower large HDL, greater intermediate and small HDL; an effect that was more pronounced in white women than black women. There were no differences in antioxidant capacity or anti-inflammatory function across groups. CONCLUSIONS: Our data show that both obesity and ethnicity are associated with differences in HDL functionality, while obesity was associated with decreases in large HDL subclass distribution. Measuring HDL functionality and subclass may, therefore, be important factors to consider when assessing cardiovascular risk. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-016-0257-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4866302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48663022016-05-14 Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution Woudberg, Nicholas J. Goedecke, Julia H. Blackhurst, Dee Frias, Miguel James, Richard Opie, Lionel H. Lecour, Sandrine Lipids Health Dis Research BACKGROUND: Obesity and low high-density lipoprotein-cholesterol (HDL-C) levels are associated with cardiovascular risk. Surprisingly, despite a greater prevalence of obesity and lower HDL concentrations than white women, black South African women are relatively protected against ischaemic heart disease. METHODS: We investigated whether this apparent discrepancy may be related to different HDL function and subclass distribution in black and white, normal-weight and obese South African women (n = 40). HDL functionality was assessed by measuring paraoxonase (PON) activity, platelet activating factor acetylhydrolase (PAF-AH) activity, Oxygen Radical Absorbance Capacity (ORAC) and quantification of the expression of vascular cell adhesion molecule in endothelial cells. PON-1 and PAF-AH expression was determined in isolated HDL and serum using Western blotting. Levels of large, intermediate and small HDL subclasses were measured using the Lipoprint® system. RESULTS: PON activity was lower in white compared to black women (0.49 ± 0.09 U/L vs 0.78 ± 0.10 U/L, p < 0.05), regardless of PON-1 protein levels. Obese black women had lower PAF-AH activity (9.34 ± 1.15 U/L vs 13.89 ± 1.21 U/L, p <0.05) and HDL-associated PAF-AH expression compared to obese white women. Compared to normal-weight women, obese women had lower large HDL, greater intermediate and small HDL; an effect that was more pronounced in white women than black women. There were no differences in antioxidant capacity or anti-inflammatory function across groups. CONCLUSIONS: Our data show that both obesity and ethnicity are associated with differences in HDL functionality, while obesity was associated with decreases in large HDL subclass distribution. Measuring HDL functionality and subclass may, therefore, be important factors to consider when assessing cardiovascular risk. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-016-0257-9) contains supplementary material, which is available to authorized users. BioMed Central 2016-05-11 /pmc/articles/PMC4866302/ /pubmed/27169717 http://dx.doi.org/10.1186/s12944-016-0257-9 Text en © Woudberg et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Woudberg, Nicholas J. Goedecke, Julia H. Blackhurst, Dee Frias, Miguel James, Richard Opie, Lionel H. Lecour, Sandrine Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution |
title | Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution |
title_full | Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution |
title_fullStr | Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution |
title_full_unstemmed | Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution |
title_short | Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution |
title_sort | association between ethnicity and obesity with high-density lipoprotein (hdl) function and subclass distribution |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866302/ https://www.ncbi.nlm.nih.gov/pubmed/27169717 http://dx.doi.org/10.1186/s12944-016-0257-9 |
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