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Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population

BACKGROUND: Apolipoprotein E (APOE) plays a major role in lipid metabolism and inflammation. However, the association between APOE gene polymorphisms and serum triglyceride levels remains controversial. We tested the effects of APOE variants on triglyceride levels and their interactions with the inf...

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Autores principales: Wu, Semon, Hsu, Lung-An, Teng, Ming-Sheng, Lin, Jeng-Feng, Chou, Hsin-Hua, Lee, Ming-Cheng, Wu, Yi-Ming, Su, Cheng-Wen, Ko, Yu-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866423/
https://www.ncbi.nlm.nih.gov/pubmed/27177774
http://dx.doi.org/10.1186/s12944-016-0262-z
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author Wu, Semon
Hsu, Lung-An
Teng, Ming-Sheng
Lin, Jeng-Feng
Chou, Hsin-Hua
Lee, Ming-Cheng
Wu, Yi-Ming
Su, Cheng-Wen
Ko, Yu-Lin
author_facet Wu, Semon
Hsu, Lung-An
Teng, Ming-Sheng
Lin, Jeng-Feng
Chou, Hsin-Hua
Lee, Ming-Cheng
Wu, Yi-Ming
Su, Cheng-Wen
Ko, Yu-Lin
author_sort Wu, Semon
collection PubMed
description BACKGROUND: Apolipoprotein E (APOE) plays a major role in lipid metabolism and inflammation. However, the association between APOE gene polymorphisms and serum triglyceride levels remains controversial. We tested the effects of APOE variants on triglyceride levels and their interactions with the inflammatory marker C-reactive protein (CRP) in a Taiwanese population. METHODS: Two APOE single nucleotide polymorphisms (SNPs) rs429358 and rs7412 were genotyped by TaqMan Assay using real time PCR in 595 healthy subjects attending the clinic for routine visits. RESULTS: After adjustment for clinical covariates, subjects carrying the rs429358-TT genotype and non-ε4 alleles were found to have higher CRP levels, whereas those with rs7412-CC genotype and non-ε2 alleles had significantly higher total and low-density lipoprotein cholesterol levels (all P < 0.01). Using subgroup and interaction analyses, we observed significantly lower triglyceride levels in subjects carrying the rs429358-TT genotype and non-ε4 alleles in the low CRP group (P = 2.71× 10(−4) and P = 4.32 × 10(−4), respectively), but not in those in the high CRP group (interaction P = 0.013 and 0.045, respectively). In addition, multivariate stepwise linear regression analysis showed that subjects carrying the rs429358-TT genotype and non-ε4 alleles with low CRP levels had significantly lower triglyceride levels (P < 0.001 and P < 0.001, respectively). In addition, when combined with the risk alleles of GCKR, APOA5 and LPL gene variants, we observed that triglyceride levels increased significantly with the number of risk alleles (P = 2.9 × 10(−12)). CONCLUSIONS: The combination of SNPs and ε alleles at the APOE locus is involved in managing lipid and CRP levels in the Taiwanese population. APOE polymorphisms interact with CRP to regulate triglyceride levels, thus triglyceride concentration is influenced by both the genetic background of the APOE locus and the inflammatory status of a subject. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-016-0262-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-48664232016-05-14 Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population Wu, Semon Hsu, Lung-An Teng, Ming-Sheng Lin, Jeng-Feng Chou, Hsin-Hua Lee, Ming-Cheng Wu, Yi-Ming Su, Cheng-Wen Ko, Yu-Lin Lipids Health Dis Research BACKGROUND: Apolipoprotein E (APOE) plays a major role in lipid metabolism and inflammation. However, the association between APOE gene polymorphisms and serum triglyceride levels remains controversial. We tested the effects of APOE variants on triglyceride levels and their interactions with the inflammatory marker C-reactive protein (CRP) in a Taiwanese population. METHODS: Two APOE single nucleotide polymorphisms (SNPs) rs429358 and rs7412 were genotyped by TaqMan Assay using real time PCR in 595 healthy subjects attending the clinic for routine visits. RESULTS: After adjustment for clinical covariates, subjects carrying the rs429358-TT genotype and non-ε4 alleles were found to have higher CRP levels, whereas those with rs7412-CC genotype and non-ε2 alleles had significantly higher total and low-density lipoprotein cholesterol levels (all P < 0.01). Using subgroup and interaction analyses, we observed significantly lower triglyceride levels in subjects carrying the rs429358-TT genotype and non-ε4 alleles in the low CRP group (P = 2.71× 10(−4) and P = 4.32 × 10(−4), respectively), but not in those in the high CRP group (interaction P = 0.013 and 0.045, respectively). In addition, multivariate stepwise linear regression analysis showed that subjects carrying the rs429358-TT genotype and non-ε4 alleles with low CRP levels had significantly lower triglyceride levels (P < 0.001 and P < 0.001, respectively). In addition, when combined with the risk alleles of GCKR, APOA5 and LPL gene variants, we observed that triglyceride levels increased significantly with the number of risk alleles (P = 2.9 × 10(−12)). CONCLUSIONS: The combination of SNPs and ε alleles at the APOE locus is involved in managing lipid and CRP levels in the Taiwanese population. APOE polymorphisms interact with CRP to regulate triglyceride levels, thus triglyceride concentration is influenced by both the genetic background of the APOE locus and the inflammatory status of a subject. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-016-0262-z) contains supplementary material, which is available to authorized users. BioMed Central 2016-05-13 /pmc/articles/PMC4866423/ /pubmed/27177774 http://dx.doi.org/10.1186/s12944-016-0262-z Text en © Wu et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wu, Semon
Hsu, Lung-An
Teng, Ming-Sheng
Lin, Jeng-Feng
Chou, Hsin-Hua
Lee, Ming-Cheng
Wu, Yi-Ming
Su, Cheng-Wen
Ko, Yu-Lin
Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population
title Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population
title_full Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population
title_fullStr Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population
title_full_unstemmed Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population
title_short Interactive effects of C-reactive protein levels on the association between APOE variants and triglyceride levels in a Taiwanese population
title_sort interactive effects of c-reactive protein levels on the association between apoe variants and triglyceride levels in a taiwanese population
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866423/
https://www.ncbi.nlm.nih.gov/pubmed/27177774
http://dx.doi.org/10.1186/s12944-016-0262-z
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