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The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population
BACKGROUND: Peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) is a transcription factor with a key role in adipocyte differentiation, lipid storage and glucose homeostasis. The Ala allele of the common Pro12Ala polymorphism in the isoform PPAR-γ2 is at the center of many controversies bec...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866427/ https://www.ncbi.nlm.nih.gov/pubmed/27239321 http://dx.doi.org/10.1186/s40608-016-0104-6 |
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author | Mato, Edith Pascale M. Pokam-Fosso, Priscille Eunice Atogho-Tiedeu, Barbara Noubiap, Jean Jacques N. Evehe, Marie-Solange Djokam-Dadjeu, Rosine Donfack, Olivier Sontsa Ngwa, Elvis Ndonwi Guewo-Fokeng, Magellan Mbacham, Wilfred F. Sobngwi, Eugene Mbanya, Jean Claude |
author_facet | Mato, Edith Pascale M. Pokam-Fosso, Priscille Eunice Atogho-Tiedeu, Barbara Noubiap, Jean Jacques N. Evehe, Marie-Solange Djokam-Dadjeu, Rosine Donfack, Olivier Sontsa Ngwa, Elvis Ndonwi Guewo-Fokeng, Magellan Mbacham, Wilfred F. Sobngwi, Eugene Mbanya, Jean Claude |
author_sort | Mato, Edith Pascale M. |
collection | PubMed |
description | BACKGROUND: Peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) is a transcription factor with a key role in adipocyte differentiation, lipid storage and glucose homeostasis. The Ala allele of the common Pro12Ala polymorphism in the isoform PPAR-γ2 is at the center of many controversies because in some populations, it has been observed to be associated with T2DM or obesity but, not in others. The aim of this study was to investigate the association of Pro12Ala polymorphism in the PPAR-γ2 gene with susceptibility to obesity or T2DM in a Cameroonian population. METHODS: This case-control study included 62 obese, 60 T2DM patients and 120 controls (60 non obese and 60 patients without T2DM), all unrelated and of Cameroonian origin. PPAR-γ2 was examined by genotyping for Pro12Ala using the Restriction Fragment Length Polymorphism - Polymerase Chain Reaction (PCR - RFLP). RESULTS: A portion of the 270 base pair bands of the PPAR-γ2 gene was successfully amplified. The Ala12 variant was totally absent from the study population, all participants being homozygote Pro/Pro. CONCLUSION: PPAR-γ2 Pro12Ala gene polymorphism may not be associated with obesity and T2DM. These results suggest that, PPAR-γ2 is unlikely a major gene for obesity or T2DM in the study population. |
format | Online Article Text |
id | pubmed-4866427 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48664272016-05-27 The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population Mato, Edith Pascale M. Pokam-Fosso, Priscille Eunice Atogho-Tiedeu, Barbara Noubiap, Jean Jacques N. Evehe, Marie-Solange Djokam-Dadjeu, Rosine Donfack, Olivier Sontsa Ngwa, Elvis Ndonwi Guewo-Fokeng, Magellan Mbacham, Wilfred F. Sobngwi, Eugene Mbanya, Jean Claude BMC Obes Research Article BACKGROUND: Peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) is a transcription factor with a key role in adipocyte differentiation, lipid storage and glucose homeostasis. The Ala allele of the common Pro12Ala polymorphism in the isoform PPAR-γ2 is at the center of many controversies because in some populations, it has been observed to be associated with T2DM or obesity but, not in others. The aim of this study was to investigate the association of Pro12Ala polymorphism in the PPAR-γ2 gene with susceptibility to obesity or T2DM in a Cameroonian population. METHODS: This case-control study included 62 obese, 60 T2DM patients and 120 controls (60 non obese and 60 patients without T2DM), all unrelated and of Cameroonian origin. PPAR-γ2 was examined by genotyping for Pro12Ala using the Restriction Fragment Length Polymorphism - Polymerase Chain Reaction (PCR - RFLP). RESULTS: A portion of the 270 base pair bands of the PPAR-γ2 gene was successfully amplified. The Ala12 variant was totally absent from the study population, all participants being homozygote Pro/Pro. CONCLUSION: PPAR-γ2 Pro12Ala gene polymorphism may not be associated with obesity and T2DM. These results suggest that, PPAR-γ2 is unlikely a major gene for obesity or T2DM in the study population. BioMed Central 2016-05-12 /pmc/articles/PMC4866427/ /pubmed/27239321 http://dx.doi.org/10.1186/s40608-016-0104-6 Text en © Mato et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Mato, Edith Pascale M. Pokam-Fosso, Priscille Eunice Atogho-Tiedeu, Barbara Noubiap, Jean Jacques N. Evehe, Marie-Solange Djokam-Dadjeu, Rosine Donfack, Olivier Sontsa Ngwa, Elvis Ndonwi Guewo-Fokeng, Magellan Mbacham, Wilfred F. Sobngwi, Eugene Mbanya, Jean Claude The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population |
title | The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population |
title_full | The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population |
title_fullStr | The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population |
title_full_unstemmed | The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population |
title_short | The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population |
title_sort | pro12ala polymorphism in the ppar-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a cameroonian population |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866427/ https://www.ncbi.nlm.nih.gov/pubmed/27239321 http://dx.doi.org/10.1186/s40608-016-0104-6 |
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