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High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta
Endogenous retroviruses (ERVs) are proviral phases of exogenous retroviruses that have co-evolved with vertebrate genomes for millions of years. Previous studies have identified the envelope (env) protein genes of retroviral origin preferentially expressed in the placenta which suggests a role in pl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866760/ https://www.ncbi.nlm.nih.gov/pubmed/27176223 http://dx.doi.org/10.1371/journal.pone.0155603 |
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author | Stefanetti, Valentina Marenzoni, Maria Luisa Passamonti, Fabrizio Cappelli, Katia Garcia-Etxebarria, Koldo Coletti, Mauro Capomaccio, Stefano |
author_facet | Stefanetti, Valentina Marenzoni, Maria Luisa Passamonti, Fabrizio Cappelli, Katia Garcia-Etxebarria, Koldo Coletti, Mauro Capomaccio, Stefano |
author_sort | Stefanetti, Valentina |
collection | PubMed |
description | Endogenous retroviruses (ERVs) are proviral phases of exogenous retroviruses that have co-evolved with vertebrate genomes for millions of years. Previous studies have identified the envelope (env) protein genes of retroviral origin preferentially expressed in the placenta which suggests a role in placentation based on their membrane fusogenic capacity and therefore they have been named syncytins. Until now, all the characterized syncytins have been associated with three invasive placentation types: the endotheliochorial (Carnivora), the synepitheliochorial (Ruminantia), and the hemochorial placentation (human, mouse) where they play a role in the syncytiotrophoblast formation. The purpose of the present study was to evaluate whether EqERV env RNA is expressed in horse tissues as well and investigate if the horse, possessing an epitheliochorial placenta, has “captured” a common retroviral env gene with syncytin-like properties in placental tissues. Interestingly, although in the equine placenta there is no syncytiotrophoblast layer at the maternal-fetal interface, our results showed that EqERV env RNA is highly expressed at that level, as expected for a candidate syncytin-like gene but with reduced abundance in the other somatic tissues (nearly 30-fold lower) thus suggesting a possible role in the placental tissue. Although the horse is one of the few domestic animals with a sequenced genome, few studies have been conducted about the EqERV and their expression in placental tissue has never been investigated. |
format | Online Article Text |
id | pubmed-4866760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48667602016-05-18 High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta Stefanetti, Valentina Marenzoni, Maria Luisa Passamonti, Fabrizio Cappelli, Katia Garcia-Etxebarria, Koldo Coletti, Mauro Capomaccio, Stefano PLoS One Research Article Endogenous retroviruses (ERVs) are proviral phases of exogenous retroviruses that have co-evolved with vertebrate genomes for millions of years. Previous studies have identified the envelope (env) protein genes of retroviral origin preferentially expressed in the placenta which suggests a role in placentation based on their membrane fusogenic capacity and therefore they have been named syncytins. Until now, all the characterized syncytins have been associated with three invasive placentation types: the endotheliochorial (Carnivora), the synepitheliochorial (Ruminantia), and the hemochorial placentation (human, mouse) where they play a role in the syncytiotrophoblast formation. The purpose of the present study was to evaluate whether EqERV env RNA is expressed in horse tissues as well and investigate if the horse, possessing an epitheliochorial placenta, has “captured” a common retroviral env gene with syncytin-like properties in placental tissues. Interestingly, although in the equine placenta there is no syncytiotrophoblast layer at the maternal-fetal interface, our results showed that EqERV env RNA is highly expressed at that level, as expected for a candidate syncytin-like gene but with reduced abundance in the other somatic tissues (nearly 30-fold lower) thus suggesting a possible role in the placental tissue. Although the horse is one of the few domestic animals with a sequenced genome, few studies have been conducted about the EqERV and their expression in placental tissue has never been investigated. Public Library of Science 2016-05-13 /pmc/articles/PMC4866760/ /pubmed/27176223 http://dx.doi.org/10.1371/journal.pone.0155603 Text en © 2016 Stefanetti et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Stefanetti, Valentina Marenzoni, Maria Luisa Passamonti, Fabrizio Cappelli, Katia Garcia-Etxebarria, Koldo Coletti, Mauro Capomaccio, Stefano High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta |
title | High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta |
title_full | High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta |
title_fullStr | High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta |
title_full_unstemmed | High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta |
title_short | High Expression of Endogenous Retroviral Envelope Gene in the Equine Fetal Part of the Placenta |
title_sort | high expression of endogenous retroviral envelope gene in the equine fetal part of the placenta |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866760/ https://www.ncbi.nlm.nih.gov/pubmed/27176223 http://dx.doi.org/10.1371/journal.pone.0155603 |
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