Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing

Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas. We obtained the complete genome of a CVA isolate (CVA-TA) using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The newl...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jiawei, Zhai, Ying, Liu, Weizhen, Dhingra, Amit, Pappu, Hanu R., Liu, Qingzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Viruses
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866854/
https://www.ncbi.nlm.nih.gov/pubmed/27174277
http://dx.doi.org/10.1128/genomeA.00364-16
_version_ 1782431978095640576
author Wang, Jiawei
Zhai, Ying
Liu, Weizhen
Dhingra, Amit
Pappu, Hanu R.
Liu, Qingzhong
author_facet Wang, Jiawei
Zhai, Ying
Liu, Weizhen
Dhingra, Amit
Pappu, Hanu R.
Liu, Qingzhong
author_sort Wang, Jiawei
collection PubMed
description Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas. We obtained the complete genome of a CVA isolate (CVA-TA) using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The newly identified 5′-untranslated region (5′-UTR) from CVA-TA may form additional hairpin and loop structures to stabilize the CVA genome.
format Online
Article
Text
id pubmed-4866854
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-48668542016-05-18 Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing Wang, Jiawei Zhai, Ying Liu, Weizhen Dhingra, Amit Pappu, Hanu R. Liu, Qingzhong Genome Announc Viruses Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas. We obtained the complete genome of a CVA isolate (CVA-TA) using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The newly identified 5′-untranslated region (5′-UTR) from CVA-TA may form additional hairpin and loop structures to stabilize the CVA genome. American Society for Microbiology 2016-05-12 /pmc/articles/PMC4866854/ /pubmed/27174277 http://dx.doi.org/10.1128/genomeA.00364-16 Text en Copyright © 2016 Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Viruses
Wang, Jiawei
Zhai, Ying
Liu, Weizhen
Dhingra, Amit
Pappu, Hanu R.
Liu, Qingzhong
Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing
title Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing
title_full Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing
title_fullStr Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing
title_full_unstemmed Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing
title_short Structure and Genome Organization of Cherry Virus A (Capillovirus, Betaflexiviridae) from China Using Small RNA Sequencing
title_sort structure and genome organization of cherry virus a (capillovirus, betaflexiviridae) from china using small rna sequencing
topic Viruses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866854/
https://www.ncbi.nlm.nih.gov/pubmed/27174277
http://dx.doi.org/10.1128/genomeA.00364-16
work_keys_str_mv AT wangjiawei structureandgenomeorganizationofcherryvirusacapillovirusbetaflexiviridaefromchinausingsmallrnasequencing
AT zhaiying structureandgenomeorganizationofcherryvirusacapillovirusbetaflexiviridaefromchinausingsmallrnasequencing
AT liuweizhen structureandgenomeorganizationofcherryvirusacapillovirusbetaflexiviridaefromchinausingsmallrnasequencing
AT dhingraamit structureandgenomeorganizationofcherryvirusacapillovirusbetaflexiviridaefromchinausingsmallrnasequencing
AT pappuhanur structureandgenomeorganizationofcherryvirusacapillovirusbetaflexiviridaefromchinausingsmallrnasequencing
AT liuqingzhong structureandgenomeorganizationofcherryvirusacapillovirusbetaflexiviridaefromchinausingsmallrnasequencing

Ejemplares similares