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Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells

FcγRIIIa (CD16) is a low-affinity Fc receptor of IgG. As the idio-binding receptor of IgG Fc, it plays an important role in the antibody-dependent cellular cytotoxicity of natural killer cells. The aim of the present study was to investigate the distribution of Kupffer cells (KCs) and the expression...

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Autores principales: LI, XIU-YUN, WU, LUN, LI, SHENG-WEI, ZHOU, WEN-BO, WANG, MENG-YUAN, ZUO, GUO-QING, LIU, CHANG-AN, DING, XIONG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866971/
https://www.ncbi.nlm.nih.gov/pubmed/27082928
http://dx.doi.org/10.3892/ijmm.2016.2561
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author LI, XIU-YUN
WU, LUN
LI, SHENG-WEI
ZHOU, WEN-BO
WANG, MENG-YUAN
ZUO, GUO-QING
LIU, CHANG-AN
DING, XIONG
author_facet LI, XIU-YUN
WU, LUN
LI, SHENG-WEI
ZHOU, WEN-BO
WANG, MENG-YUAN
ZUO, GUO-QING
LIU, CHANG-AN
DING, XIONG
author_sort LI, XIU-YUN
collection PubMed
description FcγRIIIa (CD16) is a low-affinity Fc receptor of IgG. As the idio-binding receptor of IgG Fc, it plays an important role in the antibody-dependent cellular cytotoxicity of natural killer cells. The aim of the present study was to investigate the distribution of Kupffer cells (KCs) and the expression of their surface receptor FcγRIIIa in hepatocellular carcinoma. Furthermore, we also aimed to observe the functional mechanism of FcγRIIIa. Immunohistochemical analysis was employed to study KCs and FcγRIIIa. In order to explore the role of FcγRIIIa in the growth of cancer cells, KCs and H22 tumor cells were co-cultured in different serum. The mRNA expression levels of tumor necrosis factor (TNF)-α and FcγRIIIa were analyzed by RT-qPCR; the TNF-α and FcγRIIIa protein expression levels were examined by enzyme-linked immunosorbent assay and western blot analysis, respectively. Our results showed that the number of Kuppfer cells in cancerous tissues (21.6±7.8) was lower than those in para-cancerous (68.8±9.1) tissues and adjacent normal hepatic tissues (62.0±1.9) (P<0.01); this decreased with the reduction in the differentiation degree of cancer (P<0.05). FcγRIIIa-positive cells were similar in morphology to KCs, and their distributive tendency was coincident (P<0.05). The increase in CD16a mRNA levels in the group treated with immune serum was 3.9-, 4.9- and 3.9-fold greater than that in the ordinary serum group at different time points, and CD16a protein expression also markedly increased (P<0.05). However, these effects were inhibited by the addition of anti-IgG Fc serum (P<0.05). The results of the present study suggested that FcγRIIIa resided in KCs, and it contributed to the inhibition of the growth of liver tumor cells.
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spelling pubmed-48669712016-05-20 Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells LI, XIU-YUN WU, LUN LI, SHENG-WEI ZHOU, WEN-BO WANG, MENG-YUAN ZUO, GUO-QING LIU, CHANG-AN DING, XIONG Int J Mol Med Articles FcγRIIIa (CD16) is a low-affinity Fc receptor of IgG. As the idio-binding receptor of IgG Fc, it plays an important role in the antibody-dependent cellular cytotoxicity of natural killer cells. The aim of the present study was to investigate the distribution of Kupffer cells (KCs) and the expression of their surface receptor FcγRIIIa in hepatocellular carcinoma. Furthermore, we also aimed to observe the functional mechanism of FcγRIIIa. Immunohistochemical analysis was employed to study KCs and FcγRIIIa. In order to explore the role of FcγRIIIa in the growth of cancer cells, KCs and H22 tumor cells were co-cultured in different serum. The mRNA expression levels of tumor necrosis factor (TNF)-α and FcγRIIIa were analyzed by RT-qPCR; the TNF-α and FcγRIIIa protein expression levels were examined by enzyme-linked immunosorbent assay and western blot analysis, respectively. Our results showed that the number of Kuppfer cells in cancerous tissues (21.6±7.8) was lower than those in para-cancerous (68.8±9.1) tissues and adjacent normal hepatic tissues (62.0±1.9) (P<0.01); this decreased with the reduction in the differentiation degree of cancer (P<0.05). FcγRIIIa-positive cells were similar in morphology to KCs, and their distributive tendency was coincident (P<0.05). The increase in CD16a mRNA levels in the group treated with immune serum was 3.9-, 4.9- and 3.9-fold greater than that in the ordinary serum group at different time points, and CD16a protein expression also markedly increased (P<0.05). However, these effects were inhibited by the addition of anti-IgG Fc serum (P<0.05). The results of the present study suggested that FcγRIIIa resided in KCs, and it contributed to the inhibition of the growth of liver tumor cells. D.A. Spandidos 2016-06 2016-04-12 /pmc/articles/PMC4866971/ /pubmed/27082928 http://dx.doi.org/10.3892/ijmm.2016.2561 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
LI, XIU-YUN
WU, LUN
LI, SHENG-WEI
ZHOU, WEN-BO
WANG, MENG-YUAN
ZUO, GUO-QING
LIU, CHANG-AN
DING, XIONG
Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
title Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
title_full Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
title_fullStr Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
title_full_unstemmed Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
title_short Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
title_sort effect of cd16a, the surface receptor of kupffer cells, on the growth of hepatocellular carcinoma cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4866971/
https://www.ncbi.nlm.nih.gov/pubmed/27082928
http://dx.doi.org/10.3892/ijmm.2016.2561
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