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Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the prese...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Urmia University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867033/ https://www.ncbi.nlm.nih.gov/pubmed/27226883 |
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author | Azad-Tirgan, Mahboobeh Sarrafzadeh-Rezaei, Farshid Malekinejad, Hassan Hobbenaghi, Rahim Heshmatian, Behnam |
author_facet | Azad-Tirgan, Mahboobeh Sarrafzadeh-Rezaei, Farshid Malekinejad, Hassan Hobbenaghi, Rahim Heshmatian, Behnam |
author_sort | Azad-Tirgan, Mahboobeh |
collection | PubMed |
description | Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the present study, we investigated potentials of fibroblast like synoviocytes (FLSs) in tendon healing. Sixteen rabbits were divided randomly into control and treatment groups. One rabbit was used as a donor of synovial membrane (synovium). The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon (DDFT). Subsequently, the tendon stumps were sutured with 3/0 nylon. In treatment group, 0.1 mL phosphate-buffered saline (PBS) solution containing 1 × 10(6) nucleated cells of FLSs was injected intratendinously at both tendon stumps just next to incision line. In control group, 0.1 mL PBS without FLSs was used for intratendinous injection. Model animals were euthanized at eight weeks, DDFTs were harvested and prepared for biomechanical study. Results of study showed that, there was no significant differences in biomechanical parameters values between FLSs treated and control groups. In conclusion, intratendinous injection of FLSs did not improve biomechanical properties during eight weeks in rabbit. |
format | Online Article Text |
id | pubmed-4867033 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Urmia University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-48670332016-05-25 Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study Azad-Tirgan, Mahboobeh Sarrafzadeh-Rezaei, Farshid Malekinejad, Hassan Hobbenaghi, Rahim Heshmatian, Behnam Vet Res Forum Original Article Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the present study, we investigated potentials of fibroblast like synoviocytes (FLSs) in tendon healing. Sixteen rabbits were divided randomly into control and treatment groups. One rabbit was used as a donor of synovial membrane (synovium). The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon (DDFT). Subsequently, the tendon stumps were sutured with 3/0 nylon. In treatment group, 0.1 mL phosphate-buffered saline (PBS) solution containing 1 × 10(6) nucleated cells of FLSs was injected intratendinously at both tendon stumps just next to incision line. In control group, 0.1 mL PBS without FLSs was used for intratendinous injection. Model animals were euthanized at eight weeks, DDFTs were harvested and prepared for biomechanical study. Results of study showed that, there was no significant differences in biomechanical parameters values between FLSs treated and control groups. In conclusion, intratendinous injection of FLSs did not improve biomechanical properties during eight weeks in rabbit. Urmia University Press 2016 2016-03-15 /pmc/articles/PMC4867033/ /pubmed/27226883 Text en © 2016 Urmia University. All rights reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Azad-Tirgan, Mahboobeh Sarrafzadeh-Rezaei, Farshid Malekinejad, Hassan Hobbenaghi, Rahim Heshmatian, Behnam Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study |
title | Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study |
title_full | Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study |
title_fullStr | Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study |
title_full_unstemmed | Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study |
title_short | Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study |
title_sort | evaluation of tendon healing using fibroblast like synoviocytes in rabbits: a biomechanical study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867033/ https://www.ncbi.nlm.nih.gov/pubmed/27226883 |
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