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Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study

Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the prese...

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Autores principales: Azad-Tirgan, Mahboobeh, Sarrafzadeh-Rezaei, Farshid, Malekinejad, Hassan, Hobbenaghi, Rahim, Heshmatian, Behnam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867033/
https://www.ncbi.nlm.nih.gov/pubmed/27226883
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author Azad-Tirgan, Mahboobeh
Sarrafzadeh-Rezaei, Farshid
Malekinejad, Hassan
Hobbenaghi, Rahim
Heshmatian, Behnam
author_facet Azad-Tirgan, Mahboobeh
Sarrafzadeh-Rezaei, Farshid
Malekinejad, Hassan
Hobbenaghi, Rahim
Heshmatian, Behnam
author_sort Azad-Tirgan, Mahboobeh
collection PubMed
description Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the present study, we investigated potentials of fibroblast like synoviocytes (FLSs) in tendon healing. Sixteen rabbits were divided randomly into control and treatment groups. One rabbit was used as a donor of synovial membrane (synovium). The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon (DDFT). Subsequently, the tendon stumps were sutured with 3/0 nylon. In treatment group, 0.1 mL phosphate-buffered saline (PBS) solution containing 1 × 10(6) nucleated cells of FLSs was injected intratendinously at both tendon stumps just next to incision line. In control group, 0.1 mL PBS without FLSs was used for intratendinous injection. Model animals were euthanized at eight weeks, DDFTs were harvested and prepared for biomechanical study. Results of study showed that, there was no significant differences in biomechanical parameters values between FLSs treated and control groups. In conclusion, intratendinous injection of FLSs did not improve biomechanical properties during eight weeks in rabbit.
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spelling pubmed-48670332016-05-25 Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study Azad-Tirgan, Mahboobeh Sarrafzadeh-Rezaei, Farshid Malekinejad, Hassan Hobbenaghi, Rahim Heshmatian, Behnam Vet Res Forum Original Article Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the present study, we investigated potentials of fibroblast like synoviocytes (FLSs) in tendon healing. Sixteen rabbits were divided randomly into control and treatment groups. One rabbit was used as a donor of synovial membrane (synovium). The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon (DDFT). Subsequently, the tendon stumps were sutured with 3/0 nylon. In treatment group, 0.1 mL phosphate-buffered saline (PBS) solution containing 1 × 10(6) nucleated cells of FLSs was injected intratendinously at both tendon stumps just next to incision line. In control group, 0.1 mL PBS without FLSs was used for intratendinous injection. Model animals were euthanized at eight weeks, DDFTs were harvested and prepared for biomechanical study. Results of study showed that, there was no significant differences in biomechanical parameters values between FLSs treated and control groups. In conclusion, intratendinous injection of FLSs did not improve biomechanical properties during eight weeks in rabbit. Urmia University Press 2016 2016-03-15 /pmc/articles/PMC4867033/ /pubmed/27226883 Text en © 2016 Urmia University. All rights reserved. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Azad-Tirgan, Mahboobeh
Sarrafzadeh-Rezaei, Farshid
Malekinejad, Hassan
Hobbenaghi, Rahim
Heshmatian, Behnam
Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
title Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
title_full Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
title_fullStr Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
title_full_unstemmed Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
title_short Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
title_sort evaluation of tendon healing using fibroblast like synoviocytes in rabbits: a biomechanical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867033/
https://www.ncbi.nlm.nih.gov/pubmed/27226883
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