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First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District

Since 2012, three viruses, known as equine hepacivirus (EqHV), equine pegivirus (EPgV) and Theiler’s disease-associated virus (TDAV), have been discovered in equines. Given that these viruses are the newest members of the Flaviviridae family, genomic information concerning circulating EqHV, EPgV and...

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Autores principales: Lu, Gang, Sun, Lingshuang, Xu, Tao, He, Dong, Wang, Zengchao, Ou, Shudan, Jia, Kun, Yuan, Liguo, Li, Shoujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868292/
https://www.ncbi.nlm.nih.gov/pubmed/27182887
http://dx.doi.org/10.1371/journal.pone.0155662
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author Lu, Gang
Sun, Lingshuang
Xu, Tao
He, Dong
Wang, Zengchao
Ou, Shudan
Jia, Kun
Yuan, Liguo
Li, Shoujun
author_facet Lu, Gang
Sun, Lingshuang
Xu, Tao
He, Dong
Wang, Zengchao
Ou, Shudan
Jia, Kun
Yuan, Liguo
Li, Shoujun
author_sort Lu, Gang
collection PubMed
description Since 2012, three viruses, known as equine hepacivirus (EqHV), equine pegivirus (EPgV) and Theiler’s disease-associated virus (TDAV), have been discovered in equines. Given that these viruses are the newest members of the Flaviviridae family, genomic information concerning circulating EqHV, EPgV and TDAV strains around the world is limited. To date, no genetic surveillance studies have been performed on these three viruses in the equine population of China. Here, a total of 177 serum samples were collected from equines across China between 2014 and 2015. Using PCR, we detected viral RNA in the serum samples, six of which were EqHV positive and two of which were EPgV positive. Co-infection with the two viruses was not observed among the Chinese equines studied, and TDAV RNA was not detected in the equine serum samples collected for this study. Phylogenetic analysis of partial NS5B open reading frame (ORF), NS3 ORF, and 5’ untranslated region nucleotide sequences from EqHV as well as partial NS3 ORF sequence from EPgV indicated that EqHV and EPgV have evolved into two main clades by themselves, both of which are circulating in China. Based on the partial NS5B and NS3 ORF sequences of EqHV, the sequences of one clade were also split into two subclades. This study enriches our knowledge of the geographic distribution of these three equine viruses.
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spelling pubmed-48682922016-05-26 First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District Lu, Gang Sun, Lingshuang Xu, Tao He, Dong Wang, Zengchao Ou, Shudan Jia, Kun Yuan, Liguo Li, Shoujun PLoS One Research Article Since 2012, three viruses, known as equine hepacivirus (EqHV), equine pegivirus (EPgV) and Theiler’s disease-associated virus (TDAV), have been discovered in equines. Given that these viruses are the newest members of the Flaviviridae family, genomic information concerning circulating EqHV, EPgV and TDAV strains around the world is limited. To date, no genetic surveillance studies have been performed on these three viruses in the equine population of China. Here, a total of 177 serum samples were collected from equines across China between 2014 and 2015. Using PCR, we detected viral RNA in the serum samples, six of which were EqHV positive and two of which were EPgV positive. Co-infection with the two viruses was not observed among the Chinese equines studied, and TDAV RNA was not detected in the equine serum samples collected for this study. Phylogenetic analysis of partial NS5B open reading frame (ORF), NS3 ORF, and 5’ untranslated region nucleotide sequences from EqHV as well as partial NS3 ORF sequence from EPgV indicated that EqHV and EPgV have evolved into two main clades by themselves, both of which are circulating in China. Based on the partial NS5B and NS3 ORF sequences of EqHV, the sequences of one clade were also split into two subclades. This study enriches our knowledge of the geographic distribution of these three equine viruses. Public Library of Science 2016-05-16 /pmc/articles/PMC4868292/ /pubmed/27182887 http://dx.doi.org/10.1371/journal.pone.0155662 Text en © 2016 Lu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lu, Gang
Sun, Lingshuang
Xu, Tao
He, Dong
Wang, Zengchao
Ou, Shudan
Jia, Kun
Yuan, Liguo
Li, Shoujun
First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District
title First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District
title_full First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District
title_fullStr First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District
title_full_unstemmed First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District
title_short First Description of Hepacivirus and Pegivirus Infection in Domestic Horses in China: A Study in Guangdong Province, Heilongjiang Province and Hong Kong District
title_sort first description of hepacivirus and pegivirus infection in domestic horses in china: a study in guangdong province, heilongjiang province and hong kong district
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868292/
https://www.ncbi.nlm.nih.gov/pubmed/27182887
http://dx.doi.org/10.1371/journal.pone.0155662
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