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Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®)
Colorectal cancer (CRC) is one of the major causes of cancer-related death and reliable blood-based prognostic biomarkers are urgently needed. The enumeration and molecular characterization of circulating tumor cells (CTCs) has gained increasing interest in clinical practice. CTC detection by CellSe...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868337/ https://www.ncbi.nlm.nih.gov/pubmed/27182774 http://dx.doi.org/10.1371/journal.pone.0155126 |
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author | Gorges, Tobias M. Stein, Alexander Quidde, Julia Hauch, Siegfried Röck, Katharina Riethdorf, Sabine Joosse, Simon A. Pantel, Klaus |
author_facet | Gorges, Tobias M. Stein, Alexander Quidde, Julia Hauch, Siegfried Röck, Katharina Riethdorf, Sabine Joosse, Simon A. Pantel, Klaus |
author_sort | Gorges, Tobias M. |
collection | PubMed |
description | Colorectal cancer (CRC) is one of the major causes of cancer-related death and reliable blood-based prognostic biomarkers are urgently needed. The enumeration and molecular characterization of circulating tumor cells (CTCs) has gained increasing interest in clinical practice. CTC detection by CellSearch(®) has already been correlated to an unfavorable outcome in metastatic CRC. However, the CTC detection rate in mCRC disease is low compared to other tumor entities. Thus, the use of alternative (or supplementary) assays might help to itemize the prognostic use of CTCs as blood-based biomarkers. In this study, blood samples from 47 mCRC patients were screened for CTCs using the FDA-cleared CellSearch(®) technology and / or the AdnaTest(®). 38 samples could be processed in parallel. We demonstrate that a combined analysis of CellSearch(®) and the AdnaTest(®) leads to an improved detection of CTCs in our mCRC patient cohort (positivity rate CellSearch(®) 33%, AdnaTest(®) 30%, combined 50%). While CTCs detected with the CellSearch(®) system were significantly associated with progression-free survival (p = 0.046), a significant correlation regarding overall survival could be only seen when both assays were combined (p = 0.013). These findings could help to establish improved tools to detect CTCs as on-treatment biomarkers for clinical routine in future studies. |
format | Online Article Text |
id | pubmed-4868337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48683372016-05-26 Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®) Gorges, Tobias M. Stein, Alexander Quidde, Julia Hauch, Siegfried Röck, Katharina Riethdorf, Sabine Joosse, Simon A. Pantel, Klaus PLoS One Research Article Colorectal cancer (CRC) is one of the major causes of cancer-related death and reliable blood-based prognostic biomarkers are urgently needed. The enumeration and molecular characterization of circulating tumor cells (CTCs) has gained increasing interest in clinical practice. CTC detection by CellSearch(®) has already been correlated to an unfavorable outcome in metastatic CRC. However, the CTC detection rate in mCRC disease is low compared to other tumor entities. Thus, the use of alternative (or supplementary) assays might help to itemize the prognostic use of CTCs as blood-based biomarkers. In this study, blood samples from 47 mCRC patients were screened for CTCs using the FDA-cleared CellSearch(®) technology and / or the AdnaTest(®). 38 samples could be processed in parallel. We demonstrate that a combined analysis of CellSearch(®) and the AdnaTest(®) leads to an improved detection of CTCs in our mCRC patient cohort (positivity rate CellSearch(®) 33%, AdnaTest(®) 30%, combined 50%). While CTCs detected with the CellSearch(®) system were significantly associated with progression-free survival (p = 0.046), a significant correlation regarding overall survival could be only seen when both assays were combined (p = 0.013). These findings could help to establish improved tools to detect CTCs as on-treatment biomarkers for clinical routine in future studies. Public Library of Science 2016-05-16 /pmc/articles/PMC4868337/ /pubmed/27182774 http://dx.doi.org/10.1371/journal.pone.0155126 Text en © 2016 Gorges et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gorges, Tobias M. Stein, Alexander Quidde, Julia Hauch, Siegfried Röck, Katharina Riethdorf, Sabine Joosse, Simon A. Pantel, Klaus Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®) |
title | Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®) |
title_full | Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®) |
title_fullStr | Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®) |
title_full_unstemmed | Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®) |
title_short | Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch(®) System and the AdnaTest(®) |
title_sort | improved detection of circulating tumor cells in metastatic colorectal cancer by the combination of the cellsearch(®) system and the adnatest(®) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868337/ https://www.ncbi.nlm.nih.gov/pubmed/27182774 http://dx.doi.org/10.1371/journal.pone.0155126 |
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